上海口腔医学

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (5): 405-409.

• 基础研究 • 上一篇    下一篇

长链非编码RNA MALAT1在舌鳞状细胞癌中的表达及生物学意义

张珊珊1, 2, 杨宏宇1, 王宇帆1, 杨辉俊1, 沈时岳1, 王锋1, 谢舒乐1, 2, 金龙1   

  1. 1.北京大学深圳医院 口腔颌面外科,广东 深圳 518036；
    2.汕头大学医学院,广东 汕头 515041
  • 收稿日期:2015-01-26 修回日期:2015-03-26 出版日期:2015-10-20 发布日期:2015-11-03
  • 通讯作者: 杨宏宇,Tel：0755-83923333-5630,E-mail：hyyang192@hotmail.com
  • 作者简介:张珊珊（1986-）,女,硕士研究生,E-mail：phyorna@126.com

Expression of long non-coding RNA MALAT1 gene in tongue squamous cell carcinoma and cell lines and its biological significance after knockdown the expression of MALAT1

ZHANG Shan-shan1, 2, YANG Hong-yu1, WANG Yu-fan1, YANG Hui-jun1, SHEN Shi-yue1, WANG Feng1, XIE Shu-le1, 2, JIN Long1   

  1. 1.Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital. Shenzhen 518036;
    2.Shantou University Medical College. Shantou 515041, Guangdong Province, China
  • Received:2015-01-26 Revised:2015-03-26 Online:2015-10-20 Published:2015-11-03

摘要： 目的： 探讨MALAT1在人舌鳞状细胞癌组织及细胞株中的表达及生物学意义。方法通过实时荧光定量PCR,应用GraphPad.Prism.v5.0软件包检测MALAT1在60例舌鳞状上皮细胞癌组织中及SCC9、SCC15、SCC25、CAL27 4株鳞状细胞癌细胞系中的表达;利用生物公司构建的慢病毒干扰载体GV248建立舌鳞状细胞癌稳转细胞株,通过生物基因芯片分析,应用GraphPad.Prism.v5.0软件包检测凋亡相关基因BNIP3L、NRG1的表达,并进行统计学处理。结果MALAT1基因在舌鳞状细胞癌组织及4株舌鳞状细胞癌细胞株中的表达较对照组显著增高（P<0.05）;经沉默MALAT1后,CAL27及SCC25中MALAT1基因的沉默效率较阴性组降低75%以上;凋亡相关基因BNIP3L、NRG1表达显著下调。结论MALAT1基因在舌鳞状细胞癌组织和细胞株中高表达;下调MALAT1基因表达后,引起肿瘤凋亡相关基因BNIP3L、NRG1的表达下调,MALTA1有望成为新的肿瘤治疗靶点。

关键词: MALAT1, 舌, 鳞状细胞癌, BNIP3L, NRG1

Abstract: PURPOSE:To explore the expression of MALAT1 in tongue squamous cell carcinoma and cell lines and its biological significance after knockdown the expression of MALAT1. METHODS: Real-time PCR was used to detect the expression of MALAT1 in tongue squamous cell carcinoma and cell lines SCC9, SCC15, SCC25 and CAL27. The expression of MALAT1 was knocked down by the lentivirus expression vectors GV248 built by biological company in 4 cell lines, which were handed over to another biological company for analysis by gene chip with GraphPad. Prism.v5.0 software. RESULTS: Compared with the control group, the expression of MALAT1 was significantly higher in tongue squamous cell carcinoma tissues and 4 cell lines. After transfection, the expression of MALAT1 was shown more than 75% decrease in CAL27 and SCC25 cell lines. The expression of apoptosis associated genes BNIP3L, NRG1 was down regulated. CONCLUSIONS: MALAT1 gene can be a marker as tumor and regulate the expression of apoptosis associated genes BNIP3L, NRG1, which can be a potential target for treatment of tongue squamous cell carcinoma.