BTG1在78例口腔鳞癌中的表达及临床意义

doi:10.19439/j.sjos.2021.02.006

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (2): 140-144.doi: 10.19439/j.sjos.2021.02.006

BTG1在78例口腔鳞癌中的表达及临床意义

靳能皓¹, 南欣荣^1,2, 闫星泉²

1.山西医科大学口腔医学院,山西太原 030001;
2.山西医科大学第一医院口腔科,山西太原 030001

收稿日期:2019-09-05 修回日期:2020-01-19 出版日期:2021-04-25 发布日期:2021-05-11
通讯作者: 南欣荣,E-mail：xr-nan@sina.com
作者简介:靳能皓（1994-）,男,在读硕士研究生,E-mail：545192726@qq.com
基金资助:
山西省重点研发计划项目（201803D31094）

Expression and clinical significance of BTG-1 in 78 patients with oral squamous cell carcinoma

JIN Neng-hao¹, NAN Xin-rong^1,2, YAN Xing-quan²

1. Shanxi Medical University School of Stomatology. Taiyuan 030001;
2. Department of Stomatology, The First Hospital of Shanxi Medical University. Taiyuan 030001, Shanxi Province, China

Received:2019-09-05 Revised:2020-01-19 Online:2021-04-25 Published:2021-05-11

摘要/Abstract

摘要： 目的:探讨B细胞易位基因1(BTG1)在口腔鳞状细胞癌中的表达及临床意义。方法:采用免疫组织化学染色检测78例口腔鳞癌组织、78例癌旁组织、20例正常口腔黏膜组织及80例颈淋巴结中BTG1蛋白的表达水平;使用蛋白免疫印迹及实时荧光定量PCR分别检测78例口腔鳞癌组织及癌旁组织中BTG1蛋白及mRNA的表达水平。采用SPSS 21.0软件包对数据进行统计学分析,包括Kaplan-Meier生存分析、Log rank检验及Cox回归分析。结果:口腔鳞癌组织及颈部阳性淋巴结中BTG1的表达水平显著低于癌旁正常组织及阴性淋巴结,低分化口腔鳞癌中BTG1的表达水平显著低于高分化鳞癌（P<0.05）。生存分析显示,BTG1低表达组无进展生存期（PFS）、总体生存时间（OS）显著低于高表达组（P<0.05）。Cox回归分析显示,肿瘤分化程度、颈淋巴结转移情况及BTG1表达均是影响口腔鳞癌预后的危险因素。结论:BTG1在口腔鳞癌中低表达,其表达与口腔鳞癌的TNM分期、分化程度有关。

关键词: B细胞易位基因1, BTG1, 口腔鳞状细胞癌

Abstract: PURPOSE: To investigate the expression and clinical significance of B cell translocation gene 1 (BTG1) in oral squamous cell carcinoma(OSCC). METHODS: Immunohistochemical staining was used to detect the expression of BTG1 protein in 78 cases of OSCC tissues, 78 adjacent tissues, 20 normal oral mucosa tissues, and 80 cervical lymph nodes. Western blot and real-time quantitative PCR were used to detect BTG1 protein and mRNA expression levels in 78 OSCC tissues and adjacent tissues. Kaplan-Meier survival analysis, Log rank test and Cox regression analysis were performed with SPSS 21.0 software package. RESULTS: The expression level of BTG1 in OSCC and cervical positive lymph nodes was significantly lower than that in normal tissues and negative lymph nodes adjacent to the cancer, and the expression of BTG1 in poorly differentiated OSCC was significantly lower than that in highly differentiated OSCC (P<0.05); Survival analysis showed the progression-free survival (PFS) and overall survival time(OS) of BTG1 low-expression group were significantly lower than those of high-expression group (P<0.05). Cox regression analysis showed that the degree of tumor differentiation, cervical lymph node metastasis, and BTG1 expression all affected patients' prognosis. CONCLUSIONS: BTG1 is lowerly expressed in OSCC, with expression related to TNM stage and differentiation of OSCC but no relation with gender, age, and tumor location, including that BTG1 may be involved in the occurrence, development and prognosis of OSCC.

Key words: B-cell translocation gene 1, BTG1, Oral squamous cell carcinoma

中图分类号:

R782

靳能皓, 南欣荣, 闫星泉. BTG1在78例口腔鳞癌中的表达及临床意义[J]. 上海口腔医学, 2021, 30(2): 140-144.

JIN Neng-hao, NAN Xin-rong, YAN Xing-quan. Expression and clinical significance of BTG-1 in 78 patients with oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2021, 30(2): 140-144.

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BTG1在78例口腔鳞癌中的表达及临床意义

Expression and clinical significance of BTG-1 in 78 patients with oral squamous cell carcinoma

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