HIST1H2BH在头颈部鳞状细胞癌中的表达及临床意义

doi:10.19439/j.sjos.2021.06.008

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (6): 599-605.doi: 10.19439/j.sjos.2021.06.008

HIST1H2BH在头颈部鳞状细胞癌中的表达及临床意义

王守鹏^1,2,*, 丁紫雪^2,*, 孟箭^1,2,3, 李梦^2,3, 李晓东^1,2, 袁壮^2,3

1.潍坊医学院口腔医学院,山东潍坊 261053;
2.徐州市中心医院口腔科,江苏徐州 221009;
3.徐州医科大学口腔医学院,江苏徐州 221004

收稿日期:2020-05-18 修回日期:2020-07-06 发布日期:2022-03-09
通讯作者: 孟箭,E-mail: mrocket@126.com
作者简介:王守鹏（1995-）,男,在读硕士研究生,E-mail: wshoupeng@126.com;丁紫雪（1992-）,女,医师,硕士, E-mail: dingzixue@126.com。^*并列第一作者
基金资助:
国家口腔疾病临床医学研究中心开放课题（NCRCO-202101）; 徐州市重点研发计划（KC21187）

Expression and clinical significance of HIST1H2BH in head and neck squamous cell carcinoma

WANG Shou-peng^1,2, DING Zi-xue², MENG Jian^2,3, LI Meng^2,3, LI Xiao-dong^1,2, YUAN Zhuang^2,3

1. School of Stomatology, Weifang Medical University. Weifang 261053, Shandong Province;
2. Department of Stomatology, Central Hospital of Xuzhou City. Xuzhou 221009, Jiangsu Province;
3. School of Stomatology, Xuzhou Medical University. Xuzhou 221004, Jiangsu Province, China

Received:2020-05-18 Revised:2020-07-06 Published:2022-03-09

摘要/Abstract

摘要： 目的: 探讨HIST1H2BH在头颈部鳞状细胞癌（head and neck squamous cell carcinoma, HNSCC）中的表达及临床意义。方法: 利用Oncomine数据库和GEPIA数据库,分析HIST1H2BH在不同类型肿瘤、HNSCC中的表达情况,以及与患者临床病理参数的关系和预后价值。采用免疫组织化学染色法分析临床标本中HIST1H2BH的表达,通过cBioPortal数据库分析HIST1H2BH在HNSCC中的变异及与预后的关系;通过String数据库,初步探讨HIST1H2BH的相互作用蛋白及分子机制。采用SPSS 24.0软件包对数据进行统计学分析。结果: Oncomine数据库和GEPIA数据库分析显示,HIST1H2BH在全身多种肿瘤中呈高表达,在HNSCC中显著高表达,且其表达水平与HNSCC患者的病理分期和预后密切相关,HIST1H2BH高表达的患者,预后较差。临床标本的免疫组织化学染色结果显示,HIST1H2BH在HNSCC患者病灶组织中的表达显著高于癌旁组织。cBioPortal数据库分析显示,HIST1H2BH在HNSCC患者中变异率不高,但与其无病生存率有一定关联。String数据库分析显示,与HIST1H2BH相关联的基因较多,其参与的生物学过程的信号通路也较复杂。结论: HIST1H2BH在HNSCC组织中的表达高于正常组织,其高表达与患者不良预后相关,可作为HNSCC诊断与预后的候选指标。

关键词: HIST1H2BH, 头颈部, 鳞状细胞癌, 数据库分析, 预后

Abstract: PURPOSE: The purpose of this study was to investigate the expression of HIST1H2BH in head and neck squamous cell carcinoma (HNSCC) and to analyze its clinical significance. METHODS: The expression of HIST1H2BH in different tumors and HNSCC were analyzed based on the Oncomine and GEPIA database, and its relationship with clinicopathological parameters and prognostic value of HNSCC patients were also analyzed. The expression of HIST1H2BH in clinical specimens were detected by immunohistochemical staining. The variation of HIST1H2BH in HNSCC and its relationship with prognosis were analyzed based on the cBioPortal database. The interaction proteins and molecular mechanism of HIST1H2BH were preliminarily predicted using the String database. SPSS 24.0 software package was used for statistical analysis of the data. RESULTS: Analysis of Oncomine database and GEPIA database showed that HIST1H2BH was highly expressed in variety of tumors, and was significantly highly expressed in HNSCC. The expression level of HIST1H2BH was closely related to the pathological stage and prognosis of HNSCC patients, the prognosis of patients with high HIST1H2BH expression was poor. Immunohistochemical results of clinical specimens showed that the expression of HIST1H2BH in the cancerous tissue of HNSCC patients was significantly higher than that in the adjacent tissues. cBioPortal database analysis showed that the variation rate of HIST1H2BH in HNSCC patients was not high, but it was related to the disease-free survival rate. String database analysis showed that there were many genes associated with HIST1H2BH, and the signal pathways involved in biological processes were also complex. CONCLUSIONS: The expression of HIST1H2BH is elevated in HNSCC tissues, and its high expression is associated with poor prognosis. HIST1H2BH could be served as a marker for diagnosis and prognosis of HNSCC.

Key words: HIST1H2BH, Head and neck, Squamous cell carcinoma, Database analysis, Prognosis

中图分类号:

R739.8

王守鹏, 丁紫雪, 孟箭, 李梦, 李晓东, 袁壮. HIST1H2BH在头颈部鳞状细胞癌中的表达及临床意义[J]. 上海口腔医学, 2021, 30(6): 599-605.

WANG Shou-peng, DING Zi-xue, MENG Jian, LI Meng, LI Xiao-dong, YUAN Zhuang. Expression and clinical significance of HIST1H2BH in head and neck squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2021, 30(6): 599-605.

参考文献

[1] Ferlay J, Soerjomataram I, Dikshit R, et al.Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012[J]. Int J Cancer, 2015, 136(5): E359-E386.
[2] Husain N, Neyaz A.Human papillomavirus associated head and neck squamous cell carcinoma: controversies and new concepts[J]. J Oral Biol Craniofac Res, 2017, 7(3): 198-205.
[3] Esteller M.Cancer epigenomics: DNA methylomes and histone-modification maps[J]. Nat Rev Genet, 2007,8(4): 286-298.
[4] Berdasco M, Esteller M.Aberrant epigenetic landscape in cancer: how cellular identity goes awry[J]. Dev Cell, 2010, 19(5): 698-711.
[5] Dawson MA, Kouzarides T.Cancer epigenetics: from mechanism to therapy[J]. Cell, 2012, 150(1): 12-27.
[6] Hema KN, Smitha T, Sheethal HS, et al.Epigenetics in oral squamous cell carcinoma[J]. J Oral Maxillofac Pathol, 2017, 21(2): 252-259.
[7] Bennett RL, Bele A, Small EC, et al.A mutation in histone H2B represents a new class of oncogenic driver[J]. Cancer Discov, 2019,9(10):1438-1451.
[8] Li X, Tian R, Gao H, et al.Identification of a histone family gene signature for predicting the prognosis of cervical cancer patients[J]. Sci Rep, 2017, 7(1): 16495.
[9] Schlingemann J, Habtemichael N, Ittrich C, et al.Patient-based cross-platform comparison of oligonucleotide microarray expression profiles[J]. Lab Invest, 2005, 85(8): 1024-1039.
[10] Pyeon D, Newton MA, Lambert PF, et al.Fundamental differences in cell cycle deregulation in human papillomavirus-positive and human papillomavirus-negative head/neck and cervical cancers[J]. Cancer Res, 2007, 67(10): 4605-4619.
[11] Stucky A, Sedghizadeh PP, Mahabady S, et al.Single-cell genomic analysis of head and neck squamous cell carcinoma[J]. Oncotarget, 2017, 8(42): 73208-73218.
[12] Leemans CR, Braakhuis BJ, Brakenhoff RH.The molecular biology of head and neck cancer[J]. Nat Rev Cancer, 2011, 11(1): 9-22.
[13] Lampri ES, Chondrogiannis G, Ioachim E, et al.Biomarkers of head and neck cancer, tools or a gordian knot?[J]. Int J Clin Exp Med, 2015, 8(7): 10340-10357.
[14] Van Waes C, Musbahi O.Genomics and advances towards precision medicine for head and neck squamous cell carcinoma[J]. Laryngoscope Investig Otolaryngol, 2017, 2(5): 310-319.
[15] Jenuwein T, Allis CD.Translating the histone code[J]. Science, 2001, 293(5532): 1074-1080.
[16] Nacev BA, Feng L, Bagert JD, et al.The expanding landscape of 'oncohistone' mutations in human cancers[J]. Nature, 2019, 567(7749): 473-478.
[17] Monteiro FL, Vitorino R, Wang J, et al.The histone H2A isoform Hist2h2ac is a novel regulator of proliferation and epithelial-mesenchymal transition in mammary epithelial and in breast cancer cells[J]. Cancer Lett, 2017, 396(1): 42-52.
[18] Bhattacharya S, Reddy D, Jani V, et al.Histone isoform H2A1H promotes attainment of distinct physiological states by altering chromatin dynamics[J]. Epigenetics Chromatin, 2017,10(1):48-56.
[19] Chen YW, Kao SY, Wang HJ, et al.Histone modification patterns correlate with patient outcome in oral squamous cell carcinoma[J]. Cancer, 2013, 119(24): 4259-4267.
[20] Le JM, Squarize CH, Castilho RM.Histone modifications: Targeting head and neck cancer stem cells[J]. World J Stem Cells, 2014, 6(5): 511-525.
[21] Leipold A, Hess J, Zaoui K.The epigenome. Target for innovative therapies in head and neck carcinoma[J]. HNO, 2015, 63(11):786-791.

HIST1H2BH在头颈部鳞状细胞癌中的表达及临床意义

Expression and clinical significance of HIST1H2BH in head and neck squamous cell carcinoma

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	董淋升, 唐进, 程维, 冉婧璇, 刘兆博, 李雅冬. IL-6和β-catenin在口腔鳞癌中的表达及预后价值[J]. 上海口腔医学, 2023, 32(3): 280-286.
[2]	朱江, 许腾, 宋冬惠, 吴森斌. 口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析[J]. 上海口腔医学, 2022, 31(5): 517-522.
[3]	叶璞, 黄兴鸿, 张伟, 吴萌, 俞辉明. cN0期舌鳞癌患者颈淋巴结转移的相关因素及预后分析[J]. 上海口腔医学, 2022, 31(5): 540-543.
[4]	夏佳琳, 曾奕玮, 赵小妹, 侯黎莉. 心理干预对头颈部恶性肿瘤患者负性情绪及生活质量影响的meta分析[J]. 上海口腔医学, 2022, 31(3): 330-336.
[5]	陈祺, 谢非, 季颖颖, 魏魁杰, 任强. LLY-283靶向PRMT5抑制头颈部鳞癌增殖和转移的实验研究[J]. 上海口腔医学, 2022, 31(2): 142-147.
[6]	陈娟, 曹若妍, 刘洋, 洪鹏宇, 唐瞻贵. TNFAIP6在口腔疣状癌与口腔鳞状细胞癌中的表达及其与临床病理的关系[J]. 上海口腔医学, 2022, 31(2): 167-172.
[7]	林阳, 蒋位财, 邱宇, 高炳菊, 李军, 陈鹏, 林李嵩. 基于SEER数据库的颊黏膜鳞状细胞癌的预后列线图构建[J]. 上海口腔医学, 2021, 30(6): 644-648.
[8]	贾舸, 于淼, 尚新华, 吴建华, 翟沁凯. 根管治疗慢性根尖周炎5年预后及影响因素分析[J]. 上海口腔医学, 2021, 30(2): 124-128.
[9]	靳能皓, 南欣荣, 闫星泉. BTG1在78例口腔鳞癌中的表达及临床意义[J]. 上海口腔医学, 2021, 30(2): 140-144.
[10]	王科, 彭国光, 谭玉莲, 何善志, 罗翠芬. miR-99a对口腔鳞癌增殖能力的影响[J]. 上海口腔医学, 2021, 30(1): 44-49.
[11]	黄丹妮, 陈文鑫, 熊浩峰, 胡鑫, 毛婷, 苏彤. 口腔鳞状细胞癌患者术后服用二甲双胍对预后的影响[J]. 上海口腔医学, 2021, 30(1): 61-65.
[12]	郑晶, 李天客, 包阳, 张素欣. Spindly和Bub3在口腔鳞状细胞癌中的表达及意义[J]. 上海口腔医学, 2020, 29(5): 528-532.
[13]	齐鑫, 谢敬生, 王梓欣. 腮腺保留式调强放疗对唾液组成、流量及口干的影响[J]. 上海口腔医学, 2020, 29(4): 396-399.
[14]	李羽, 黄文青, 陈林林. LncRNA NEAT1通过miR-339-5p/ITGA3轴调控舌鳞状细胞癌增殖、迁移及侵袭的分子机制[J]. 上海口腔医学, 2020, 29(3): 267-274.
[15]	达雨, 杨震, 夏苗苗, 王梦佳. Ki-67、PI3K、Beclin1在口腔鳞癌组织中的表达及意义[J]. 上海口腔医学, 2020, 29(1): 75-79.