Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction

Shanghai Journal of Stomatology ›› 2014, Vol. 23 ›› Issue (4): 391-396.

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Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction

ZHAO Jing-lei, JIANG ling-yong, MAO Li-xia, LIU Jia-qiang, FANG Bing

Department of Oral and Cranomaxillofacial Science, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2013-10-25 Online:2014-08-20 Published:2014-10-20
Supported by:
Supported by National Natural Science Foundation of China (81371178), Key Basic Research Foundation of Science and Technology Committee of Shanghai Municipality (12JC1405700), Collaborative Foundation of Medical and Engineering Science of Shanghai Jiao Tong University (YG2012MS40), Key Basic Research Foundation of Science and Technology Committee of Shanghai Municipality (12JC1405700), "Chen Xing" Project from Shanghai Jiao Tong University, and Innovative Research Team of Shanghai Municipal Education Commission.

Abstract

Abstract: PURPOSE: To isolate and identify the human periodontal ligament stem cells, evaluate osteogenetic capacity, and investigate the changes of osteogenic bone related gene expression in mineralized medium at different times. METHODS: PDLSCs were isolated by tissue culture and magnetic activated cell sorting. Immunofluorescence staining was used for identification. The general situation of osteogenesis was assessed with alkaline phosphatase (ALP) and alizarin red staining. Real-time PCR was used to detect the expression of genes in osteoinduction. SPSS12.0 software package was used for data analysis. RESULTS: Tissue culture with magnetic cell sorting could isolate high-purity human periodontal ligament stem cells. During the osteogenic process, the expression of FoxO1 and Runx2 firstly increased and then decreased, ALP and OCN gene levels continued to increase. CONCLUSIONS: Similar to osteogenesis, ALP, Runx2, FoxO1 and OCN are regularly expressed during osteogenic induction.

Key words: Periodontal ligament stem cell, Osteogenic induction, Osteogenic differentiation

CLC Number:

R781.4

ZHAO Jing-lei, JIANG ling-yong, MAO Li-xia, LIU Jia-qiang, FANG Bing. Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction[J]. Shanghai Journal of Stomatology, 2014, 23(4): 391-396.

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Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction

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