Biological characteristics of periodontal ligament stem cells modified by platelet derived growth factor BB

doi:10.19439/j.sjos.2020.04.002

Abstract

Abstract: PURPOSE: To investigate the biological characteristics of human periodontal stem cells (hPDLSCs) modified with platelet derived growth factor BB(PDGFBB) gene, and to explore its influence on proliferation, migration and osteogenic induction of hPDLSCs. METHODS: hPDLSCs were isolated and amplified, and immunofluorescence staining was performed to identify cell surface markers and osteogenic differentiation ability. hPDLSCs were transfected with PDGFBB gene by lentivirus vector, and the effects on cell proliferation and migration were detected by CCK-8 and scratch test after transfection. Real-time PCR was performed to analyze the mRNA expression levels of osteogenic and angiogenic genes in hPDLSCs cells transfected with PDGFBB gene. Statistical analysis was performed using SPSS 22.0 software package. RESULTS: hPDLSCs were successfully obtained by tissue mass culture and finite dilution method. Compared with the blank virus group and non-transfected group, the proliferation and migration ability of the cells in the transfection group were significantly increased, and the mRNA expression levels of OPN, COL-1 and VEGF were significantly up-regulated(P<0.05). CONCLUSIONS: Lentiviral vector can transfer PDGFBB gene into hPDLSCs in vitro and obtain continuous and stable expression. PDGFBB can promote proliferation and migration of hPDLSCs cells and up-regulate expression of osteogenic and angiogenic genes.

Key words: Bone repair, Platelet derived growth factor, Periodontal ligament stem cells, Lentiviral vector

CLC Number:

R781.4

DENG Jia-jia, PAN Jie, YU Li-ming, ZHANG Wei-hua, ZHU Lu-ying, HAN Xin-xin, LIU Yue-hua. Biological characteristics of periodontal ligament stem cells modified by platelet derived growth factor BB[J]. Shanghai Journal of Stomatology, 2020, 29(4): 343-349.

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