The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells

Abstract

Abstract: PURPOSE: To investigate the effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells (PDLCs) in vitro. METHODS: Human PDLCs were isolated and characterized. The proliferation rate of PDLCs under different concentration of CoCl2 were tested by MTT assay. The PDLCs’ osteogenic differentiation were investigated using real-time PCR and Western blot. The date was statistically analyzed with SPSS13.0 software package. RESULTS: Immunocytochemical staining verified that the isolated cells were PDLCs. The proliferation of PDLCs and the expression of alkaline phosphatase (ALP), RUNX2, collagen I were significantly decreased in a dose-dependent manner by 200 μmol/L CoCl2 and 400μmol/L CoCl2. CONCLUSIONS: Hypoxia inhibits proliferation and osteogenic differentiation of human PDLCs.

Key words: Periodontal ligament cells, Hypoxia, Cobalt chloride, Osteogenic differentiation

CLC Number:

R781.4

DONG Jia-chen, SONG Zhong-chen, SHU Rong, LI Song, LIN Zhi-kai,ZHANG Xiu-li. The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2014, 23(4): 397-401.

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