Role of P2X7 receptor in osteogenic differentiation of human periodontal ligament stem cells

doi:10.19439/j.sjos.2020.04.007

Abstract

Abstract: PURPOSE: To investigate the role of P2X7 receptor (P2X7r) in osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). METHODS: hPDLSCs were isolated from the premolars collected in the First Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, and divided into four groups. Group A was cultured in conventional medium, group B was cultured in osteogenic induction medium, group C was cultured in osteogenic induction medium + 100 nmol/L adenosine triphosphate (ATP) solution, and group D was cultured in osteogenic induction medium + 100 nmol/L P2X7 receptor specific antagonist KN-62. After 7 days, alizarin red staining was used to observe the osteogenic effect of hPDLSCs in each group. The mRNA expression of osteocalcin (OCN), RUNX2 and P2X7r in hPDLSCs was detected by real-time PCR reaction (RT-PCR). The data were processed by SPSS 22.0 software package. RESULTS: Alisarin red staining showed that the morphology of hPDLSCs cells in group B and group C was significantly changed. The pale calcified nodules in group C were significantly more than those in group B, while very few calcified nodules were found in group A and group D. The mRNA expression of OCN, RUNX2 and P2X7r in hPDLSCs were the highest in group C, followed by group B(P<0.05), and no difference was found between group A and group D(P>0.05). CONCLUSIONS: P2X7 receptor can promote osteogenic differentiation of human periodontal ligament stem cells after being activated by ATP, which may provide a new direction for clinical treatment of periodontitis.

Key words: P2X7 receptor, Adenosine triphosphate, Human periodontal ligament stem cells, Osteogenic differentiation

CLC Number:

PENG Yu-zhi, TANG Song-jiang, LI Xiao-jing, ZHAO Sheng-ke, LIU Bao-zhen. Role of P2X7 receptor in osteogenic differentiation of human periodontal ligament stem cells[J]. Shanghai Journal of Stomatology, 2020, 29(4): 370-374.

References

[1] 喻爱霞. 牙周炎患者应用牙周组织再生术联合正畸治疗的临床效果评价[J]. 河北医科大学学报, 2017, 38(3):306-310.
[2] 唐春梅, 赵会杰, 许刚, 等. 牙周组织再生术联合口腔正畸对牙周炎患者血清炎症因子水平的影响及其疗效探究[J]. 现代生物医学进展, 2017,17(20): 3965-3968.
[3] 陈发明, 高丽娜, 陈芳. 牙周再生治疗现状和进展[J]. 口腔疾病防治, 2019, 27(1): 16-23.
[4] 陈小燕, 丁寅, 金岩. TNF-α通过NF-κB信号通路对人牙周膜干细胞成骨分化调控作用的研究[J]. 口腔生物医学, 2015, 6(3): 124-128.
[5] 习德娥, 韩莉, 谭超, 等. P2X7受体与基因转录的关系[J]. 中国免疫学杂志, 2015, 15(9): 1294-1297.
[6] 李喆, 马蓓, 陈爱民. P2X7受体与骨代谢研究进展[J]. 第二军医大学学报, 2015, 36(7): 767-770.
[7] 孙亚男, 魏建敏, 孙薇, 等. P2X7受体对牙周膜干细胞成骨分化的作用[J]. 中国组织工程研究, 2015, 19(6): 861-867.
[8] 杨海静, 王永兰, 张燕, 等. 慢性牙周炎患者长期牙周治疗失访现状及影响因素研究[J]. 中国全科医学, 2018, 21(30): 42-48.
[9] Peng C, Zheng J, Chen D, et al.Response of hPDLSCs on 3D printed PCL/PLGA composite scaffolds in vitro[J]. Mol Med Rep, 2018, 18(2): 1335-1344.
[10] Ren S, Yao Y, Zhang H, et al.Aligned fibers fabricated by near-field electrospinning influence the orientation and differentiation of hPDLSCs for periodontal regeneration[J]. J Biomed Nanotechnol, 2017, 13(12): 1725-1734.
[11] 张奕,胡婷,叶国,等. p38 MAPK通路调控BMP9诱导hPDLSCs成骨分化的体外研究[J]. 上海口腔医学, 2018, 27(6): 596-601.
[12] Heupel J, Bläsche R, Wesslau KP, et al.P2X7R: independent modulation of aquaporin 5 expression in CdCl 2-injured alveolar epithelial cells[J]. Histochem Cell Biol, 2018, 149(3): 197-208.
[13] Peng K, Liu L, Wei D, et al.P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation[J]. Int J Mol Med, 2015, 35(5): 1179-1188.
[14] Fernandes NC, Sriram U, Gofman L, et al.Methamphetamine alters microglial immune function through P2X7R signaling[J]. J Neuroinflammation, 2016, 13(1): 91-95.
[15] Jiang S, Zhang Y, Zheng JH, et al.Potentiation of hepatic stellate cell activation by extracellular ATP is dependent on P2X7R-mediated NLRP3 inflammasome activation[J]. Pharmacol Res, 2017, 117: 82-93.