The effect of inflammation on proliferation and osteogenic differentiation of periodontal ligament cells

doi:10.19439/j.sjos.2022.03.004

Abstract

Abstract: PURPOSE: To investigate the effects of inflammatory microenvironment on proliferation and osteogenic differentiation of periodontal ligament cells(PDLCs) in vitro. METHODS: Human PDLCs were isolated and characterized. MTT was used to investigate the proliferation rate of PDLCs under different concentration of lipopolysaccharide（LPS）. The PDLCs' osteogenic differentiation was investigated using real-time PCR and Western blot. The date were statistically analyzed with SPSS 13.0 software package. RESULTS: Treatment with 0.1 μg/mL LPS increased proliferation of PDLCs and enhanced the expression of osteogenic gene and protein. The proliferation of PDLCs and expression of alkaline phosphatase(ALP), RUNX2, Collagen-I, BMP2 were significantly decreased by 10 μg/mL LPS. CONCLUSIONS: The inflammatory microenvironment (10 μg/mL LPS) inhibits the proliferation and osteogenic differentiation of human PDLCs.

Key words: Periodontal ligament cells, Inflammatory microenvironment, Lipopolysaccharide, Osteogenic differentiation

CLC Number:

R781.4

DONG Jia-chen, SHU Rong. The effect of inflammation on proliferation and osteogenic differentiation of periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2022, 31(3): 243-247.

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