Abstract: PURPOSE: To evaluate the effects of IL-1β on inflammatory factors synthesis of human gingival fibroblasts on different surfaces of titaniums in vitro. METHODS: Human gingival fibroblasts were cultured on 3 different kinds of titaniums. The cells were stimulated with IL-1β, and the IL-6 and IL-8 production was detected by polymerase chain reaction (PCR) after 3, 6 and 24 h. The date was statistically analyzed with SPSS13.0 software package. RESULTS: A significant increase of the expression of gingival fibroblasts to inflammatory factors (IL-6, IL-8) after IL-1β was noted; PCR results of the production of inflammatory factors in gingival fibroblasts with the intervention of IL-1β showed that the production of inflammatory factors (IL-6, IL-8) on titanium and titanium alloy were less than that on the titanium nitride. CONCLUSIONS: IL-1β can stimulate high expression of inflammatory factors; The production of IL-6 and IL-8 on titanium and titanium alloy are less, suggesting that implant made of titanium and titanium alloy can reduce the incidence of peri-implant inflammation, and improve the successful rate of implantation.

Key words: Gingival fibroblasts, IL-1β, IL-6, IL-8