Effect of photodynamic therapy on interleukin in gingival crevicular fluid from patients receiving oral implantation

doi:10.19439/j.sjos.2019.01.012

Abstract

Abstract: PURPOSE: Photodynamic therapy (PDT) has been applied in treatment of peri-implantitis for its antimicrobial effects. The aim of the study was to evaluate the effects of a Denfotex PAD Light System's photodynamic therapy on prevention of peri-implantitis. METHODS: Twenty patients with implants in molar area were treated with photoactivated disinfection right after completion of suprastructures. The irradiation time was 30 seconds for mesial, distal, buccal and lingual/palatal sites respectively at a power output of 100mW. Gingival crevicular fluid (GCF) samples from these patients were obtained before photodynamic treatment, 2 weeks and 3 months after treatment. GCF was collected using paper points, and enzyme-linked immunosorbent assay (ELISA) was performed to determine the cytokine (IL-1β, ΤΝF-α, IL-6 and ΙL-17) levels. The data were analyzed with SPSS 23.0 software package. RESULTS: ELISA showed IL-1β and ΤΝF-α levels of all patients were decreased 2 weeks and 3 months after treatment. Reduction of IL-1β, ΤΝF-α, IL-6 and IL-17 from 2 weeks to 3 months and from baseline to 3 months all showed a significant difference (P<0.05). CONCLUSIONS: Denfotex PAD^TM light system is effective in reducing peri-implant inflammatory mediators and can work as an effective treatment to prevent peri-implantitis.

Key words: Peri-implantitis, Photodynamic therapy, IL-1β, TNF-α, IL-6, IL-17

CLC Number:

R783.6

ZHOU Lin-yi, ZHANG Chu-nan, GU Ying-xin, LAI Hong-chang. Effect of photodynamic therapy on interleukin in gingival crevicular fluid from patients receiving oral implantation[J]. Shanghai Journal of Stomatology, 2019, 28(1): 63-66.

References

[1] Rocha FS, Jesus RN, Rocha FM, et al.Saliva versus peri-implant inflammation: quantification of IL-1beta in partially and totally edentulous patients[J]. J Oral Implantol, 2014, 40(2): 169-173.
[2] Tzach-Nahman R, Mizraji G, Shapira L, et al.Oral infection with Porphyromonas gingivalis induces peri-implantitis in a murine model: Evaluation of bone loss and the local inflammatory response[J]. J Clin Periodontol, 2017, 44(7): 739-748.
[3] Konermann A, Gotz W, Le M, et al.Histopathological verification of osteoimmunological mediators in peri-implantitis and correlation to bone loss and implant functional period[J]. J Oral Implantol, 2016, 42(1): 61-68.
[4] Wang HL, Garaicoa-Pazmino C, Collins A, et al.Protein biomarkers and microbial profiles in peri-implantitis[J]. Clin Oral Implants Res, 2016, 27(9): 1129-1136.
[5] Kikuchi T, Mogi M, Okabe I, et al.Adjunctive application of antimicrobial photodynamic therapy in nonsurgical periodontal treatment: a review of literature[J]. Int J Mol Sci, 2015, 16(10): 24111-24126.
[6] Dörtbudak O, Haas R, Bernhart T.Lethal photosensitization for decontamination of implant surfaces in the treatment of peri-implantitis[J]. Clin Oral Implants Res, 2001, 12(2): 104-108.
[7] Bassetti M, Schär D, Wicki B, et al.Anti-infective therapy of peri-implantitis with adjunctive local drug delivery or photodynamic therapy: 12-month outcomes of a randomized controlled clinical trial[J]. Clin Oral Implants Res, 2014, 25(3): 279-287.
[8] Mizutani K, Aoki A, Coluzzi D, et al.Lasers in minimally invasive periodontal and peri-implant therapy[J]. Periodontol 2000, 2016, 71(1): 185-212.
[9] Duarte PM, Serrao CR, Miranda TS, et al.Could cytokine levels in the peri-implant crevicular fluid be used to distinguish between healthy implants and implants with peri-implantitis? A systematic review[J]. J Periodontal Res, 2016, 51(6): 689-698.
[10] Renvert S, Widén C, Persson GR.Cytokine expression in peri-implant crevicular fluid in relation to bacterial presence[J]. J Clin Periodontol, 2015, 42(7): 697-702.
[11] Salvi GE, Aglietta M, Eick S, et al.Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans[J]. Clin Oral Implants Res, 2012, 23(2): 182-190.
[12] Hienz SA, Paliwal S, Ivanovski S.Mechanisms of bone resorption in periodontitis[J]. J Immunol Res, 2015, 2015: 615486.
[13] Kolbe MF, Ribeiro FV, Luchesi VH, et al.Photodynamic therapy during supportive periodontal care: clinical, microbiologic, immunoinflammatory, and patient-centered performance in a split-mouth randomized clinical trial[J]. J Periodontol, 2014, 85(8): e277-286.
[14] Yu JJ, Ruddy MJ, Wong GC, et al.An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals[J]. Blood, 2007, 109(9): 3794-3802.
[15] Lindén A, Laan M, Anderson GP.Neutrophils, interleukin-17A and lung disease[J]. Eur Respir J, 2005, 25(1): 159-172.
[16] Toh ML, Kawashima M, Hot A, et al.Role of IL-17 in the Th1 systemic defects in rheumatoid arthritis through selective IL-12Rbeta2 inhibition[J]. Ann Rheum Dis, 2010, 69(8): 1562-1567.
[17] Ciucci T, Ibáñez L, Boucoiran A, et al.Bone marrow Th17 TNFalpha cells induce osteoclast differentiation, and link bone destruction to IBD[J]. Gut, 2015, 64(7): 1072-1081.
[18] Konttinen YT, Laine P, Kitti U, et al.Immunohistochemical evaluation of inflammatory mediators in failing implants[J]. Int J Periodontics Restorative Dent, 2006, 26(2): 135-141.
[19] Johnson RB, Serio FG.The contribution of interleukin-13 and -15 to the cytokine network within normal and diseased gingiva[J]. J Periodontol, 2007, 78(4): 691-695.
[20] Segarra-Vidal M, Guerra-Ojeda S, Valles LS, et al.Effects of photodynamic therapy in periodontal treatment: A randomized, controlled clinical trial[J]. J Clin Periodontol, 2017, 44(9): 915-925.
[21] Beklen A, Ainola M, Hukkanen M, et al.MMPs, IL-1, and TNF are regulated by IL-17 in periodontitis[J]. J Dent Res, 2007, 86(4): 347-351.
[22] Zoccal KF, Bitencourt Cda S, Sorgi CA, et al.Ts6 and Ts2 from Tityus serrulatus venom induce inflammation by mechanisms dependent on lipid mediators and cytokine production[J]. Toxicon, 2013, 61: 1-10.
[23] Renvert S, Widén C, Persson RG.Cytokine and microbial profiles in relation to the clinical outcome following treatment of peri-implantitis[J]. Clin Oral Implants Res, 2017, 28(9): 1127-1132.
[24] de Moraes M, de Vasconcelos RC, Longo JP, et al. Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva[J]. Photodiagnosis Photodyn Ther, 2015, 12(4): 592-597.