Abstract: PURPOSE: To modify polyetheretherketone(PEEK) composite material with nanometer titanium dioxide (TiO₂) and CRGDS peptide to prepare TiO₂-PEEK-CRGDS, and evaluate its anti- activity of Porphyromonas gingivalis and effect on cell activity. METHODS: TiO₂-PEEK was prepared by plasma immersion ion implantation(PⅢ), then immersed into gelatin solution of pentapeptides of CRGDS, and freeze-dried. The same diameter and thickness of PEEK was set as control. Scanning electron microscope (SEM) was used to observe the three-dimensional topography and roughness on the surface of the specimen, colony count was calculated to evaluate the survival of Porphyromonas gingivalis, living/dead cell count was used to detect the activity of human gingival fibroblasts(HGFs), CCK-8 assay was used to detect the proliferation ability of HGFs. The quantitative data was indicated as and analyzed by using GraphPad Prism 7.0 software package. RESULTS: The antibacterial rates of PEEK, TiO₂-PEEK and TiO₂-PEEK-CRGDS were 40.3%, 57.2% and 60.3%, respectively. At 12 days, the ability of TiO₂-PEEK-CRGDS to promote the growth of human gingival fibroblasts was significantly different from that of the TiO₂-PEEK and PEEK group(P<0.05). CONCLUSIONS: The optimized TiO₂-PEEK-CRGDS has better hydrophilicity than pure titanium and PEEK, and can significantly promote the proliferation of gingival fibroblasts.

Key words: TiO₂-PEEK-CRGDS, Porphyromonas gingivalis, Human gingival fibroblasts