Cytotoxicity of 3 resin cements to human gingival fibroblasts

doi:10.19439/j.sjos.2017.06.005

Abstract

Abstract: PURPOSE: To study the cytotoxicity of 3 resin cements to human gingival fibroblasts (HGFs). METHODS: Three resin cements (Panavia F, RelyX^TM Unicem and Multilink Speed) test samples were immersed and incubated in the culture medium for 48 h at 37℃. Cultured HGFs were exposed to two concentrations (50% and 100%) of material elutes for 24 h, 72 h and 120 h. The proliferation rate was evaluated using CCK-8 assay. The data were statistically analyzed by one-way analysis of variance using SPSS 20.0 software package. RESULTS: Relative growth rate(RGR) of all experimental groups ranged from 10.67% to 100.02%, the cytotoxicity grade of all groups was 0 to 4. There was no significant difference in the RGR among 3 resin cements, but the experimental group of Panavia F (uncovered with antioxidant) showed significantly lower RGR than other experimental groups. CONCLUSIONS: Panavia F, RelyX^TM Unicem and Multilink Speed exhibit no cytotoxicity to HGFs, Panavia F(uncovered with antioxidant) shows moderate cytotoxicity.

Key words: Resin cement, Cytotoxicity, Human gingival fibroblasts

CLC Number:

R783.1

ZHANG Chang-yuan, NIU Gang, JIN Yi-yi, YU Hao, CHENG Hui. Cytotoxicity of 3 resin cements to human gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2017, 26(6): 594-598.

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