Effect of local corticotomies on mesenchymal stem cells and growth factors of bone marrow aspirate concentrate

doi:10.19439/j.sjos.2022.06.005

Abstract

Abstract: PURPOSE: This study investigated the impact of local corticotomies on mesenchymal stem cells (MSCs) and pro-regenerative growth factors contained in bone marrow aspirate concentrate(BMAC). METHODS: Five domestic pigs (Sus Scrofa), 4-5 months of age, either sex, were included. Each pig underwent operations for creation of two 1cm-long corticotomies at one randomly chosen tibia (as experimental), with the other tibia left intact (as control). At postoperative day-14, bone marrow was aspirated from both tibiae and processed into BMAC samples, from which MSCs and plasmas were separated. MSCs quantity, proliferative and osteogenic differentiation properties, as well as several regenerative growth factors contained in BMAC samples, were assessed and compared between the two sides. Statistical analysis was performed with SPSS 25.0 software package. RESULTS: Creation of corticotomy, bone marrow aspiration and corticotomy healing all occurred uneventfully. The quantity of MSCs assessed by colony-forming fibroblast unit assay and flow cytometry was significantly higher on the corticotomy side (P<0.05). MSCs from the corticotomy side also proliferated significantly faster (P<0.05) and showed a tendency of stronger osteogenic differentiation potential although only mRNA expression of osteocalcin reached statistical significance(P<0.05). The concentration of TGF-β, BMP2 and PDGF contained in BMAC tended to be higher on corticotomy side than the control side but the difference did not reach statistical significance. CONCLUSIONS: Local corticotomies are able to boost the quantity and proliferative/osteogenic differentiation property of MSC contained in BMAC.

Key words: Injury, Bone marrow aspirate concentrate, Mesenchymal stem cell, Growth factors, Tissue engineering

CLC Number:

R780.2

SHI Lei, WANG Jun. Effect of local corticotomies on mesenchymal stem cells and growth factors of bone marrow aspirate concentrate[J]. Shanghai Journal of Stomatology, 2022, 31(6): 588-596.

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