The role of Bmal1 in regulating the aging of mesenchymal stem cells via Wnt/β-catenin signaling pathway

doi:10.19439/j.sjos.2020.06.008

Abstract

Abstract: PURPOSE: To investigate whether Bmal1 and Wnt/β-catenin signaling pathway has synergistic or antagonistic effects on the aging of mouse bone marrow-derived mesenchymal stem cells (BMSCs). METHODS: The cells were divided into two groups. The expression of β - catenin and TCF1 in the transfected group and the blank group were detected by real-time fluorescent quantitative PCR and Western blot. The data were statistically analyzed using SPSS 19.0 software package. RESULTS: Recombinant Bmal1 lentiviral vector was successfully constructed. The expression of β-catenin was enhanced after Bmal1 was transfected(P<0.05), which was different from that of TCF1. There was no significant difference in TCF1. CONCLUSSIONS: Bmal1 has a synergistic effect on the aging changes of MSCs in mice directly or indirectly regulated by Wnt/β-catenin signaling pathway.

Key words: Aging, Marrow mesenchymal stem cells, Classical Wnt signaling pathway, β-catenin, Bmal1

CLC Number:

R783.5

YE Cui, WU Hai-miao. The role of Bmal1 in regulating the aging of mesenchymal stem cells via Wnt/β-catenin signaling pathway[J]. Shanghai Journal of Stomatology, 2020, 29(6): 601-605.

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