Abstract: PURPOSE: To study the physicochemical properties of bismuth-containing cross-linked alginate gel, to observe and detect the adhesion and proliferation of mouse MC3T3-E1 cells in gluten-containing cross-linked alginate gel and provides a theoretical basis for alginate injectable tissue engineering scaffolds. METHODS: Five sets of electrolytes with a final concentration of 4 g/L were prepared and cross-linked with sodium alginate solution, respectively. The compression modulus and swelling ratio of each group of gels were measured. Cells were inoculated on the surface of the composite gel, FDA/PI cells were stained to observe the adhesion morphology of the cells. Data were statistically analyzed using GraphPad Prism 6.0 software package. RESULTS: The swelling rate of double distilled water and PBS increased with the increase of Sr²⁺ concentration. The results of compression modulus showed that the strength of group C was significantly higher(P<0.05). Scanning electron microscopy showed that the pores of the composite gel increased with the increase of Sr²⁺ concentration. Microscopy and FDA/Pi staining showed that the cells were scattered in the initial stage of cell inoculation. CONCLUSIONS: The composite gel formed by mixing equal proportion of strontium ions may be used as a new type of excellent cell scaffold material in bone tissue engineering.

Key words: Alginate, Strontium, Hydrogel, Pre-osteoblasts, Tissue engineering