Effect of human bone marrow mesenchymal stem cells on invasion of tongue squamous cell carcinoma cell line Cal-27

doi:10.19439/j.sjos.2020.02.009

Abstract

Abstract: PURPOSE: To investigate the effect of human bone marrow mesenchymal stem cells (hBM-MSCs) on invasion of tongue squamous cell carcinoma cell line Cal-27 and its mechanism. METHODS: hBM-MSCs and Cal-27 were cultured respectively, and the morphology of the cells was observed under an inverted microscope. The co-cultured Cal-27 cells were obtained by co-culture of hBM-MSCs and Cal-27. The migration area of Cal-27 was observed by scratch test;transwell migration and invasion experiments were performed to observe migration and invasion of Cal-27, and a bar graph was then drawn. Fluorescence quantitative PCR was used to observe the effect of hBM-MSCs on gene expression of the tumor markers E-cadherin, twist, slug, snail, MMP-2 and MMP-9. Western blot was used to observe the effect of hBM-MSCs on protein expression of MMP-2 and MMP-9, related to the invasion of Cal-27. SPSS 19.0 software package was used for statistical analysis of the data. RESULTS: Under the influence of hBM-MSCs, the invasion of Cal-27 was promoted, accompanied by down-regulation of E-cadherin, up-regulation of twist, slug, snail, MMP-2, MMP-9 and up-regulation of MMP-2 and MMP-9 expression. CONCLUSIONS: hBM-MSCs can promote invasion of Cal-27 cells, which may be related to up-regulation of the expression of tumor markers related to invasion of Cal-27 cells.

Key words: Human bone marrow mesenchymal stem cells, Tongue squamous cell carcinoma, Invasion, Tumor markers

CLC Number:

XU Zhu-qing, ZHANG Xin-yu, WANG Shao-ru, LI Tao, DONG Gang, LI Feng-mei, ZHENG Jian-jin, WANG Yun-ying, XU Xiao-na. Effect of human bone marrow mesenchymal stem cells on invasion of tongue squamous cell carcinoma cell line Cal-27[J]. Shanghai Journal of Stomatology, 2020, 29(2): 155-161.

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