The effect of commonly used subgingival wedge-shaped defect filling materials on the formation of Porphyromonas gingivalis biofilm

doi:10.19439/j.sjos.2020.04.008

Abstract

Abstract: PURPOSE: To investigate the effect of commonly used subgingival wedge-shaped defect filling materials on the formation of Porphyromonas gingivalis (P.gingivalis) biofilm. METHODS: Subgingival class V cavities were prepared on the buccal surfaces of 48 extracted premolars. After that, the premolars were randomly divided into 3 groups (A, B and C) with 16 in each group. After filling and polishing with universal nano fluid resin, universal nano solid resin and glass ionomer cement individually, Isomet 4000 precision cutting machine was used to make specimens containing all the filling materials (mesiodistal distance: 5 mm, occlusal-gingival distance: 3 mm, buccolingual distance: 2 mm). All the specimens were placed in P.gingivalis bacterial solution for 24 hours to form P.gingivalis biofilm. Then the attachment amount of P.gingivalis was observed by crystal violet staining. Confocal laser scanning microscopy (CLSM) was used to observe the P.gingivalis biofilm. SPSS 22.0 software package was used for Kruskal-Wallis rank sum test. RESULTS: The results of crystal violet staining showed that the amount of P.gingivalis adhered to the glass ionomer cement filling surface was significantly less than that of other groups(P<0.05). CLSM scan showed no significant difference in proportion of viable bacteria and biofilm thickness among three groups (P>0.05). P.gingivalis biofilm in glass ionomer cement group was sparse, showing small clumps, and did not aggregate into pieces. On the surface of universal nano solid resin, P.gingivalis biofilm was in large pieces with a three-dimensional structure. CONCLUSIONS: Compared with the universal nano solid/fluid resin, the surface of glass ionomer cement is not conducive to the formation of P.gingivalis biofilm. Therefore, from the perspective of periodontal health, glass ionomer cement is suitable for filling treatment of subgingival wedge-shaped defects.

Key words: Subgingival wedge-shaped defect, Porphyromonas gingivalis, Biofilm, Universal nano solid resin, Universal nano fluid resin, Glass ionomer cement

CLC Number:

R781.3

MA Jian, SHAO Qiang, YU Yi. The effect of commonly used subgingival wedge-shaped defect filling materials on the formation of Porphyromonas gingivalis biofilm[J]. Shanghai Journal of Stomatology, 2020, 29(4): 375-379.

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