Preliminary study on the correlation between IL-6,IL-33, and IL-10 and periodontitis and rheumatoid arthritis

doi:10.19439/j.sjos.2021.05.010

Abstract

Abstract: PURPOSE: To determine the expression levels of interleukin-6(IL-6), interleukin-33 (IL-33) and interleukin-10 (IL-10) in gingival crevicular fluid in patients with periodontitis and rheumatoid arthritis, and to investigate the correlation between IL-6, IL-33, IL-10 and periodontitis and rheumatoid arthritis. METHODS: According to the inclusion criteria, patients who were admitted to the Department of Stomatology and Rheumatology and Immunology of Shengjing Hospital of China Medical University were selected, including 21 patients with periodontitis (PD group), 21 patients with rheumatoid arthritis (RA group), and 24 patients with periodontitis combined with rheumatoid arthritis (PD+RA group), 24 healthy patients (H group). General information for each subject was recorded including periodontal probing depth (PPD), clinical attachment loss (CAL), and gingival sulcus bleeding index (SBI). Samples of gingival crevicular fluid were collected from each group, and the contents of IL-6, IL-33 and IL-10 in gingival crevicular fluid were determined by ELISA, and the correlation between IL-6, IL-33, IL-10 and periodontal clinical indicators was analyzed. SPSS 20.0 software package was used to analyze the date. RESULTS: The expression level of IL-6 in PD+RA group was significantly higher than that in H group, PD group and RA group (P<0.05). The content of IL-33 in PD group, RA group and PD+RA group was significantly higher than that in H group (P<0.05), while the content of IL-33 in PD+RA group was significantly higher than that in RA group (P<0.05). The expression level of IL-10 in the RA group was significantly higher than that in the H group, the PD group and the PD+RA group (P<0.05). PPD in PD group was positively correlated with the contents of IL-6 and IL-33 (r=0.62, 0.43), SBI, PPD and CAL in PD+RA group were positively correlated with the contents of IL-33 (r=0.69, 0.58, 0.55). CONCLUSIONS: The levels of IL-6 and IL-33 in gingival crevicular fluid of patients with periodontitis accompanied by rheumatoid arthritis are significantly increased, while the contents of IL-10 are significantly decreased, suggesting that IL-6, IL-33 and IL-10 play an important role in the occurrence and development of periodontitis and rheumatoid arthritis.

Key words: Periodontitis, Rheumatoid arthritis, Interleukin-6, Interleukin-33, Interleukin-10, Cytokines

CLC Number:

R781.4

WANG Ye, ZHANG Li-mu, QI Shuai, LIN Xiao-ping. Preliminary study on the correlation between IL-6,IL-33, and IL-10 and periodontitis and rheumatoid arthritis[J]. Shanghai Journal of Stomatology, 2021, 30(5): 498-503.

References

[1] da Costa TA, Silva MJB, Alves PM, et al. Inflammation biomarkers of advanced disease in nongingival tissues of chronic periodontitis patients[J]. Mediators Inflamm, 2015, 2015: 983782.
[2] Smolen JS, Aletaha D, McInnes IB. Rheumatoid arthritis[J]. Lancet, 2016, 388(10055): 2023-2038.
[3] Mobini M, Maboudi A, Ali MR.Periodontitis in rheumatoid arthritis patients, abundance and association with disease activity[J]. Med J Islam Repub Iran, 2017, 31:44.
[4] Kim JH, Choi IA, Lee JY, et al.Periodontal pathogens and the association between periodontitis and rheumatoid arthritis in Korean adults[J]. J Periodontal Implant Sci, 2018, 48(6): 347-359.
[5] Rodríguez-Lozano B, González-Febles J, Garnier-Rodríguez JL, et al.Association between severity of periodontitis and clinical activity in rheumatoid arthritis patients: a case-control study[J]. Arthritis Res Ther, 2019, 21(1): 27.
[6] Kaneko C, Kobayashi T, Ito S, et al.Circulating levels of carbamylated protein and neutrophil extracellular traps are associated with periodontitis severity in patients with rheumatoid arthritis: a pilot case-control study[J]. PLoS One, 2018,13(2): e192365.
[7] Papapanou PN, Sanz M, Buduneli N, et al.Periodontitis: consensus report of workgroup 2 of the 2017 World Workshop on the classification of periodontal and peri-implant diseases and conditions[J]. J Clin Periodontol, 2018, 45(Suppl 20): S162-S170.
[8] Aletaha D, Neogi T, Silman AJ, et al.2010 Rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative[J]. Arthritis Rheum, 2010, 62(9): 2569-2581.
[9] de Oliveira FR, de Brito SR, Magno MB, et al. Does periodontitis represent a risk factor for rheumatoid arthritis? a systematic review and meta-analysis [J]. Ther Adv Musculoskelet Dis, 2019, 11: 1759720X19858514.
[10] Äyräväinen L, Leirisalo-Repo M, Kuuliala A, et al.Periodontitis in early and chronic rheumatoid arthritis: a prospective follow-up study in Finnish population[J]. BMJ Open, 2017, 7(1): e11916.
[11] Naruishi K, Nagata T.Biological effects of interleukin-6 on gingival fibroblasts: cytokine regulation in periodontitis[J]. J Cell Physiol, 2018, 233(9): 6393-6400.
[12] Balaji A, Chandrasekaran SC, Subramanium D, et al.Salivary Interleukin-6-A pioneering marker for correlating diabetes and chronic periodontitis: a comparative study[J]. Indian J Dent Res, 2017, 28(2): 133-137.
[13] Stadler AF, Angst PD, Arce RM, et al.Gingival crevicular fluid levels of cytokines/chemokines in chronic periodontitis: a meta-analysis[J]. J Clin Periodontol, 2016,43(9): 727-745.
[14] Tekeoglu I, Harman H, Sag S, et al.Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis[J]. Cytokine, 2016, 83: 171-175.
[15] Saglam M, Koseoglu S, Aral CA, et al.Increased levels of interleukin-33 in gingival crevicular fluids of patients with chronic periodontitis[J]. Odontol, 2017,105(2):184-190.
[16] Matsuyama Y, Okazaki H, Tamemoto H, et al.Increased levels of interleukin 33 in sera and synovial fluid from patients with active rheumatoid arthritis[J]. J Rheumatol, 2010, 37(1): 18-25.
[17] Malcolm J, Awang RA, Oliver-Bell J, et al.IL-33 exacerbates periodontal disease through induction of RANKL[J]. J Dent Res, 2015, 94(7): 968-975.
[18] Zhang Q, Chen B, Yan F, et al.Interleukin-10 inhibits bone resorption: a potential therapeutic strategy in periodontitis and other bone loss diseases[J]. Biomed Res Int, 2014, 2014: 284836.
[19] Yu P, Hu Y, Liu Z, et al.Local induction of B cell interleukin-10 competency alleviates inflammation and bone loss in ligature-induced experimental periodontitis in mice[J]. Infect Immun, 2016, 85(1): e00645-16.
[20] Toker H, Gorgun EP, Korkmaz EM, et al.The effects of IL-10 gene polymorphism on serum, and gingival crevicular fluid levels of IL-6 and IL-10 in chronic periodontitis[J]. J Appl Oral Sci, 2018, 26: e20170232.
[21] Guggino G, Giardina A, Ferrante A, et al.The in vitro addition of methotrexate and/or methylprednisolone determines peripheral reduction in Th17 and expansion of conventional Treg and of IL-10 producing Th17 lymphocytes in patients with early rheumatoid arthritis[J]. Rheumatol Int, 2015, 35(1): 171-175.
[22] Chen S, Chen B, Wen Z, et al.IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis[J]. Oncotarget, 2017, 8(20): 32407-32418.
[23] Hernández-Bello J, Oregón-Romero E, Vázquez-Villamar M, et al.Aberrant expression of interleukin-10 in rheumatoid arthritis: Relationship with IL10 haplotypes and autoantibodies[J]. Cytokine, 2017, 95: 88-96.
[24] Qu C H, Hou Y, Bi Y F, et al.Diagnostic values of serum IL-10 and IL-17 in rheumatoid arthritis and their correlation with serum 14-3-3eta protein[J]. Eur Rev Med Pharmacol Sci, 2019, 23(5): 1899-1906.
[25] 蔡辉, 张群燕. 类风湿关节炎患者外周血血浆中TNF-α、IL-1β及IL-10的表达及临床意义[J].中华中医药学刊, 2017, 35(3): 519-521.