Application of sulfylated calcium alginate composite hydrogel loaded with zinc-based zeolite imidazole for the treatment of periodontitis

doi:10.19439/j.sjos.2022.04.008

Abstract

Abstract: PURPOSE: To explore the feasibility of adhesive thiomer calcium alginate composite hydrogel (ZIF-8@CHA-SH) containing metal-organic framework for the treatment of periodontitis in rats. METHODS: Preparation and characterization of ZIF-8@CHA-SH were performed, and the morphology and chemical composition were observed. The antibacterial properties were detected by plate coating method and scanning electron microscope. CCK-8 and live/dead cell fluorescence staining were used to evaluate the toxicity of ZIF-8@CHA-SH on gingival fibroblasts. The anti-inflammatory and tissue repair promoting effects of ZIF-8@CHA-SH were verified by animal experiments. GraphPad Prism 7.0 software was used for statistical analysis. RESULTS: The successful synthesis of ZIF-8@CHA-SH was proved by scanning electron microscopy, energy spectrum analysis, Fourier transform infrared spectroscopy and X-ray diffraction. ZIF-8@CHA-SH had excellent antibacterial ability, and the antibacterial rates against Escherichia coli, Staphylococcus aureus and Streptococcus mutans were (99.88±0.12)%, (99.81±0.32)% and (95.53±3.08)%, respectively（P<0.001）. The cell viability rate of ZIF-8@CHA-SH was (91.64±3.66)% after 5 days of co-culture with human gingival fibroblasts (P=0.6). In vivo experiments showed that ZIF-8@CHA-SH could reduce the infiltration of inflammatory cells and the expression of pro-inflammatory factor IL-6 in rats with periodontitis. CONCLUSIONS: ZIF-8@CHA-SH has excellent biological performance, which can effectively treat periodontitis caused by bacteria, and provids a new strategy for the treatment of periodontal diseases in the future.

Key words: Hydrogel, ZIF-8, Periodontitis

CLC Number:

R781.4

OUYANG Ming, WANG Guo-hua. Application of sulfylated calcium alginate composite hydrogel loaded with zinc-based zeolite imidazole for the treatment of periodontitis[J]. Shanghai Journal of Stomatology, 2022, 31(4): 379-383.

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