Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells

doi:10.19439/j.sjos.2018.03.009

Abstract

Abstract: PURPOSE: To investigation the effect of flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells. METHODS: After evaluation of the toxic effect of chrysin, preosteoblast MC3T3-E1 cell suspension was prepared with DMEM and then the cells were cultured with 0 and 25 μmol/L chrysin. Real-time quantitative PCR was used to detect the expression of osteogenic marlcers including Runx2, ColA1 and OCN. Statistical analysis was performed using SPSS17.0 software package. RESULTS: 25 μmol/L chrysin had no toxic effect on preosteoblast MC3T3-E1 cells. After co-culture with 25 μmol/L chrysin,the expression of Runx2, ColA1 and OCN was highest at the 7th,14th and 21th day,respectively.CONCLUSIONS: : 25 μmol/L chrysin can promote osteogenic gene expression of preosteoblast MC3T3-E1 cell.

Key words: Chrysin, Osteogenic differentiation, MC3T3-E1

CLC Number:

R381.01

XIA Bing, HONG Tao, HE Xin.. Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells[J]. Shanghai Journal of Stomatology, 2018, 27(3): 261-264.

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