Effect of akebiasaponin D with different concentrations on orthodontic tooth movement in rats

doi:10.19439/j.sjos.2018.02.004

Abstract

Abstract: PURPOSE: This study investigated the effect of local injection of akebiasaponin D (ASD) with different concentrations on the rate of orthodontic tooth movement in rats. METHODS: Forty 6-week-old female Wistar rats were randomly divided into 4 groups with 10 rats in each group. Animal model for orthodontic tooth movement was established. The upper first molars of the rats were moved mesially by coil springs（force=40 g）. ASD solution at the concentration of 5 mg/kg was locally injected in ASD1 group, and ASD solution at the concentration of 10 mg/kg was locally injected in ASD2 group. The rats of group PGE2 were injected PGE2 solution at the concentration of 25 μg/kg. The same amount of normal saline was injected in the control group. The rats were sacrificed in batch on the 3rd, 7th, 14th, 21st and 28th days after orthodontic treatment. The distance between upper first molar and second molar was measured. H-E straining was performed to observe the changes of periodontal tissue and the amount of osteoclast. SPSS13.0 software package was used for statistical analysis. RESULTS: The distance between the first and second molar was successively increased compared with the control group. On day 3, there was significant difference between PGE2 group and the control group (P<0.05). On day 7, the distance between the first and second molar in PGE2 group and ASD2 group was significantly increased compared with the control group (P<0.05). The amount of tooth movement was significantly increased (P<0.05) in ASD1 group, ASD2 group and PGE2 group, compared with the control group on the 14th, 21st and 28th day. However, there was no significant difference (P>0.05) between ASD2 group and PGE2 group. Under microscope, the number of osteoclast was increased on the tension side, reaching a peak on day 21st, and decreased later. CONCLUSIONS: Local injection of ASD solution may accelerate orthodontic tooth movement. ASD solution at the dose of 10 mg/kg can accelerate orthodontic tooth movement efficiently similar to PGE2 solution, while ASD solution at the dose of 5 mg/kg is not as effectual as PGE2 solution.

Key words: Akebiasaponin D, Orthodontic tooth movement, Osteoclast

CLC Number:

R783.5

CUI Jin-jie, WANG Xu-xia, WANG Yuan, CHEN Pei-pei, MA Dan, ZHANG Jun. Effect of akebiasaponin D with different concentrations on orthodontic tooth movement in rats[J]. Shanghai Journal of Stomatology, 2018, 27(2): 129-134.

References

[1] 王梦含. 正畸牙快速移动的研究进展[J]. 现代口腔医学杂志, 2016, 30(1): 46-50.
[2] Yamasaki K, Shibata Y, Fukuhara T.The effect of prostaglandins on experimental tooth movement in monkeys (Macacafuscata)[J]. J Dent Res, 1982, 61(12): 1444-1446.
[3] Seifi M, Eslami B, Saffar AS.The effect of prostaglandin E2 and calcium gluconate on orthodontic tooth movement and root resorption in rats[J]. Eur J Orthod, 2003, 25(2): 199-204.
[4] Caglaroglu M, Erdem A.Histopathologic investigation of the effects of prostaglandin E2 administered by different methods on tooth movement and bone metabolism[J]. Korean J Orthod, 2012, 42(3): 118-128.
[5] 中华人民共和国卫生部药典委员会. 中国药典 [M]. 北京: 人民卫生出版社, 1985: 291-292.
[6] 席兰兰, 王媛, 颜淑云, 等. 灌服川续断水煎液对大鼠正畸牙移动及牙周组织中破骨细胞的影响[J]. 上海口腔医学, 2010, 19(4): 410-414.
[7] Zhou YQ, Yang ZL, Xu L, et al.Akebiasaponin D, a saponin component from Dipsacusasper Wall, protects PC 12 cells against amyloid-beta induced cytotoxicity[J]. Cell Biol Int, 2009, 33(10): 1102-1110.
[8] Li C, Liu Z, Tian J, et al.Protective roles of Asperosaponin VI, a triterpenesaponin isolated from Dipsacusasper Wall on acute myocardial infarction in rats[J]. Eur J Pharmacol, 2010, 627(1-3): 235-241.
[9] Roodman GD.Advances in bone biology: the osteoclast[J]. Endocr Rev, 1996, 17(4): 308-332.
[10] 谭洪根, 林生, 张启伟, 等. 高效液相色谱法测定续断药材中川续断皂苷Ⅵ的含量[J]. 中国中药杂志, 2006, 31(9): 726-727.
[11] Klein DC, Raisz LG.Prostaglandins: stimulation of bone resorption in tissue culture[J]. Endocrinology, 1970, 86(6): 1436-1440.
[12] Chumbley AB, Tuncay OC.The effect of indomethacin (an aspirin-like drug) on the rate of orthodontic tooth movement[J]. Am J Orthod, 1986, 89(4): 312-314.
[13] Vane JR.Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs[J]. Nat New Biol, 1971, 231(25): 232-235.
[14] Yamasaki K, Shibata Y, Imai S, et al.Clinical application of prostaglandin E1 (PGE1) upon orthodontic tooth movement[J]. Am J Orthod, 1984, 85(6): 508-518.
[15] 张丁, 傅民魁. 前列腺素E1对矫治牙移动的临床研究[J]. 实用口腔医学杂志, 1993, 9(1): 8-10.
[16] 李东, 陈华, 徐如生. 前列腺素E2加速兔牙移动的实验研究[J]. 口腔医学, 1991, 11(3): 116-118.
[17] 段银钟, 陈华, 林珠, 等. 前列腺素E2及环磷酸腺苷对兔牙加速移动的研究[J]. 华西口腔医学杂志, 1989, 7(1): 27-29.
[18] Leiker BJ, Nanda RS, Currier GF, et al.The effects of exogenous prostaglandins on orthodontic tooth movement in rats[J]. Am J Orthod Dentofacial Orthop, 1995, 108(4): 380-388.
[19] Rygh P.Ultrastructural changes in tension zones of rat molar periodontium incident to orthodontic tooth movement[J]. Am J Orthod, 1976, 70(3): 269-281.
[20] Storey E.The nature of tooth movement[J]. Am J Orthod, 1973, 63(3): 292-314.
[21] 陈雅竹, 管晓燕, 肖茜文, 等. 中草药在正畸牙移动牙周组织改建中的作用[J]. 中国组织工程研究, 2014, 18(46): 7477-7481.