Effect of TNF-α on the ability of stem cells from human exfoliated deciduous teeth to promote osteoclastogenesis

doi:10.19439/j.sjos.2018.05.001

Abstract

Abstract: PURPOSE: To investigate the effect of tumor necrosis factor-α (TNF-α) on the ability of stem cells from human exfoliated deciduous teeth (SHED) to promote osteoclastogenesis. METHODS: SHED were obtained from deciduous teeth and isolated, purified, cultured in vitro. An indirect co-culture system of SHED and osteoclast precursor peripheral blood mononuclear cells (PBMCs) was established. The expression of osteoclastic gene from PBMCs and NF-κB from SHED were determined after treatment with TNF-α (0, 5, 10, 50, 100 ng/mL) by real-time RT-PCR and Western blot. SPSS 19.0 software package was used for statistical analysis. RESULTS: Under the stimulation of 10ng/mL TNF-α, the expression of CTSK and TRAP was markedly upregulated in PBMCs. Meanwhile, the results of Western blot and real-time RT-PCR showed that the expression of cytoplasmic phosphorylated inhibitor of NF-κB α (p-IκBα) and nuclear p65 in SHED were significantly higher than that without TNF-α stimulation after 10 ng/mL TNF-α treatment. CONCLUSIONS: TNF-α regulates the ability of SHED to promote osteoclastogenesis through NF-κB signal pathways.

Key words: Stem cells from human exfoliated deciduous teeth, Tumor necrosis factor-α, Nuclear factor-κB signal pathways, Osteoclastic differentiation

CLC Number:

Q254

WANG Yu-chen, WANG Chen, LIU Na, CAI Chuan, LI Wei, XU Lu-lu. Effect of TNF-α on the ability of stem cells from human exfoliated deciduous teeth to promote osteoclastogenesis[J]. Shanghai Journal of Stomatology, 2018, 27(5): 449-454.

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