Effect of low-energy microwave irradiation on orthodontic tooth movement and periodontal tissue remodeling in rats

doi:10.19439/j.sjos.2022.02.008

Abstract

Abstract: PURPOSE: To investigate the effect of low-energy microwave irradiation on the movement of orthodontic teeth and periodontal tissue reconstruction in rats. METHODS: SD rats were randomly divided into control group and experimental group. Helical spring force method was used to construct a rat orthodontic model through a nickel-titanium tension spring device. Rats in the experimental group were irradiated with a microwave treatment apparatus once a day to move the first molars for 30 minutes, while rats in the control group were not given any intervention. The rats were sacrificed on the day of modeling, 7 d, 14 d, and 21 d thereafter. The movement distance of the rat's first molars was measured. Tartrate-resistant acid phosphatase (TRAP) staining was used to detect osteoclast counts in rat periodontal tissues, immunohistochemistry was used to detect the expression of cell differentiation factor (osteoclast differentiation factor, ODF) in rat periodontal tissues; real-time fluorescence quantitative PCR(RT-PCR) was used to detect the mRNA expression of interleukin 6(IL-6) and tumor necrosis factor-α (TNF-α). SPSS 20.0 software package was used to analyze the experimental data. RESULTS: At 7, 14, 21 d, compared with the control group, the distance of the first molar movement, the count of osteoclasts in the periodontal tissue, and the expression of ODF in the experimental group were significantly increased (P<0.05), while the mRNA expression of IL-6 and TNF-α in periodontal tissues was significantly decreased (P<0.05). CONCLUSIONS: Low-energy microwave irradiation can significantly accelerate the movement of orthodontic teeth, inhibit the expression of inflammatory genes, and promote the reconstruction of periodontal tissue.

Key words: Low-energy microwave irradiation, Orthodontic tooth movement, Periodontal tissue reconstruction, Inflammatory genes

CLC Number:

R783.5

LIU Wei-jia, TANG Zhen, ZHANG Qi, JIANG Li-ping. Effect of low-energy microwave irradiation on orthodontic tooth movement and periodontal tissue remodeling in rats[J]. Shanghai Journal of Stomatology, 2022, 31(2): 156-161.

References

[1] Robertson L, Kaur H, Fagundes NCF, et al.Effectiveness of clear aligner therapy for orthodontic treatment: a systematic review[J].Orthod Craniofac Res, 2020, 23(2): 133-142.
[2] 时函, 王洁, 武影, 等.双相磷酸钙作用下牙周组织缺损再生的Beagle犬正畸牙移动的骨改建功能[J]. 吉林大学学报(医学版), 2019, 45(5): 1025-1030.
[3] Baxter SJ, Sydorak I, Ma PX, et al.Impact of pharmacologic inhibition of tooth movement on periodontal and tooth root tissues during orthodontic force application[J]. Orthod Craniofac Res, 2020, 23(1): 35-43.
[4] Shahabee M, Shafaee H, Abtahi M, et al.Effect of micro-osteoperforation on the rate of orthodontic tooth movement-a systematic review and a meta-analysis[J]. Eur J Orthod, 2020, 42(2): 211-221.
[5] Baghizadeh Fini M, Olyaee P, Homayouni A.The effect of low-level laser therapy on the acceleration of orthodontic tooth movement[J]. J Lasers Med Sci, 2020, 11(2): 204-211.
[6] 邱媛媛, 吴清松, 张旭升, 等影像学引导物理消融及经皮骨水泥成形术治疗骨转移疼痛研究进展[J]. 中国介入影像与治疗学, 2019, 16(2): 62-65.
[7] Lee KS, Sin JM, Patil PP, et al.Ultrasound-guided microwave ablation for the management of inguinal neuralgia: a preliminary study with 1-year follow-up[J]. J Vasc Interv Radiol, 2019, 30(2): 242-248.
[8] 罗金莉, 杜希希, 袁小平. 异丙肾上腺素对大鼠牙移动过程中牙槽骨微结构的影响[J]. 安徽医科大学学报, 2019, 54(11): 1698-1701.
[9] 姜委杰, 刘珺, 林炜, 等. 不同强度的低能量激光照射对正畸牙齿移动速度影响的临床研究[J]. 全科口腔医学电子杂志, 2019, 6(7):19-21.
[10] 何蕊, 杜希希, 龙杨, 等. 异丙肾上腺素对大鼠正畸牙移动的影响[J]. 医学研究生学报, 2018, 31(11): 1158-1162.
[11] Wu D, Meng B, Cheng Y, et al.The effect of risedronate on orthodontic tooth movement in ovariectomized rats[J]. Arch Oral Biol, 2019, 105: 59-64.