Effect of overexpression of transcription factor Runx2 and Osterix on osteogenic differentiation of endothelial cells

doi:10.19439/j.sjos.2017.04.001

Abstract

Abstract: To explore the effect of overexpression of Runx2 and Osterix (OSX) genes on osteogenic differentiation of human umbilical vein endothelial cells (HUVECs). METHODS: Overexpressed Runx2 and OSX lentiviral vectors were transfected into HUVECs respectively. The osteogenic potential of transfected cells was identified by alkaline phosphatase (ALP) staining and ALP activity. Furthermore, real time-PCR, Western blot and immunofluorescence staining were performed to detect the expression of osteogenic genes and proteins in HUVECs. GraphPad Prism 6.01 software was used for statistical analysis. RESULTS: Overexpression of Runx2 gene was beneficial for osteogenic differentiation of HUVECs, while overexpression of osterix gene did not show osteogenic differential potential. Moreover, overexpression of Runx2 gene in HUVECs up-regulated the gene expression level of Runx2, OSX, ALP, bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN), and up-regulated protein level of OPN and OCN. CONCLUTIONS: Overexpression of Runx2 could promote osteogenic differentiation of HUVECs.

Key words: Runx2 gene, Osterix gene, Osteogenic differentiation, Human umbilical vein endothelial cells

CLC Number:

R739.8

YANG Guang-zheng, ZHANG Wen-jie, DING Xun, ZHANG Xiang-kai, JIANG Xin-quan, ZHANG Zhi-yuan. Effect of overexpression of transcription factor Runx2 and Osterix on osteogenic differentiation of endothelial cells[J]. Shanghai Journal of Stomatology, 2017, 26(4): 353-357.

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