Effect of NaF on proliferation and mineralization of human periodontal ligament cells

doi:10.19439/j.sjos.2017.01.010

Abstract

Abstract: PURPOSE: To investigate the effect of NaF on proliferation and mineralization of human periodontal ligament cells (hPDLCs). METHODS: hPDLCs were isolated and characterized. The proliferation of hPDLCs treated with different concentration of NaF was tested by CCK-8. Four moderate concentrations were chosen for subsequent experiments. The mineralization was investigated using ALP activity assay, Alizarin red S staining and quantitative real-time polymerase chain reaction(RT-PCR). The data were statistically analyzed with SPSS20.0 software package. RESULTS: Immunohistochemistry showed that the isolated cells were hPDLCs. 5×10^-5, 1×10^-4 and 5×10^-4 mol/L NaF had pro-proliferation effects while 5×10^-4 mol/L resulted in the best effect (P<0.05). ALP activity and calcium content was significantly enhanced by 1×10^-5 mol/L NaF with osteogenic inductive medium (P<0.05). Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. CONCLUSIONS: 5×10^-5, 1×10^-4, 5×10^-4 mol/L NaF can stimulate proliferation in hPDLCs, 1×10^-5 mol/L NaF can enhance ALP activity and calcium content formation of hPDLCs.

Key words: Fluoride, Periodontal ligament cells, Mineralization, Periodontitis

CLC Number:

R781.4

LI kun-qin, XU Le, HUANG Sheng-yun, ZHANG dong-sheng. Effect of NaF on proliferation and mineralization of human periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2017, 26(1): 48-53.

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