Ginsenoside Rg1 regulates the proliferation and migration of human periodontal ligament cells via Akt/eNOS signaling under nicotine stress

doi:10.19439/j.sjos.2017.01.009

Abstract

Abstract: PURPOSE: To explore the effects and molecular mechanisms of ginsenoside Rg1 on the proliferation and migration of human periodontal ligament cells (HPDLCs) under nicotine stress. METHODS: HPDLCs were isolated and cultured by method of explant cell culture. The cells were cultured under nicotine stress for 7 days, and treated respectively with ginsenoside Rg1 (0.01 μmol/L), ginsenoside Rg1 and LY294002 (PI3K inhibitor, 0.5 μmol/L), ginsenoside Rg1 and Tricirbine (Akt inhibitor, 5 μmol/L), ginsenoside Rg1 and L-NAME (Akt inhibitor, 1 mmol/L) from 3rd day after nicotine stress to 7th day. MTT assay and Transwell assay were used to evaluate the proliferation and migration of HPDLCs in each group. Western blot and quantitative real-time PCR methods were used for testing the changes of PI3K/Akt/eNOS signaling expression. SPSS 20.0 software package was used for statistical analysis. RESULTS: The proliferation and migration were significantly inhibited by nicotine treatment. PI3K levels were upregulated, but Akt1/2 and eNOS levels were remarkedly reduced by nicotine. Ginsenoside Rg1 attenuated the effects of nicotine on proliferation, migration and Akt/eNOS signaling. Tricirbine and L-NAME could reduce the inhibitory effects of ginsenoside Rg1 toward nicotine. CONCLUSIONS: Ginsenoside Rg1 regulates the proliferation and migration of HPDLCs under nicotine stress via Akt/eNOS signaling.

Key words: Periodontal ligament cells, Nicotine, Ginsenoside Rg1, Akt/eNOS signaling

CLC Number:

R781.4

LIU Cai-hong, DU Li. Ginsenoside Rg1 regulates the proliferation and migration of human periodontal ligament cells via Akt/eNOS signaling under nicotine stress[J]. Shanghai Journal of Stomatology, 2017, 26(1): 42-47.

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