Klf10 silencing inhibited mechanical force induced osteogenic differentiation of human periodontal ligament cells

doi:10.19439/j.sjos.2017.06.006

Abstract

Abstract: PURPOSE: To investigate the effect of Klf10 silence on human periodontal ligament cells (HPDLCs) under mechanical force. METHODS: HPDLCs were isolated and transfected with Klf10 siRNA, and then exposed to centrifugal force for 6 h at 631 r/min. Purmorphamine, an hedgehog signaling pathway agonist, was used for intervention. The activity of alkaline phosphatase (ALP) was detected by ELISA. RT-PCR and Western blot were performed to detect the mRNA and protein expression of Klf10, Runt related transcription factor 2 (Runx2), osteopontin (OPN) and osteocalcin (OCN). The protein expression of glioma associated oncogene homolog 1 (Gli1) and patched-1 (PTCH1) was detected by Western blot. SPSS 20.0 software package was used to analyze the data. RESULTS: Mechanical force increased mRNA and protein level of Klf10, Runx2, OPN and OCN, and elevated ALP activity significantly (P<0.05). Mechanical force also upregulated the protein expression of GLI1 and Ptch1 significantly (P<0.05). Compared with the control group, Klf10 siRNA transfection significantly decreased mRNA and protein level of Klf10 (P<0.05). Klf10 siRNA significantly inhibited the activity of ALP, and downregulated mRNA and protein expression of Runx2, OPN and OCN (P<0.05). Moreover, Klf10 siRNA significantly inhibited protein expression of GLI1 and Ptch1 (P<0.05), and purmorphamine obviously inhibited the effect of Klf10 siRNA (P<0.05). CONCLUSIONS: Klf10 silencing could inhibit bone differentiation of human periodontal ligament cells under mechanical force, which may be through regulation of hedgehog signaling pathway.

Key words: Orthodontics, Klf10, Human periodontal ligament cells, Mechanical force, Osteogenic differentiation

CLC Number:

R783.5

YU Xiao-qin, LI Yun-long, Gao Jin-yu, LIU Hua. Klf10 silencing inhibited mechanical force induced osteogenic differentiation of human periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2017, 26(6): 599-604.

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