长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义

doi:10.19439/j.sjos.2019.01.013

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (1): 67-70.doi: 10.19439/j.sjos.2019.01.013

长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义

杨宗澄¹, 黄海燕², 徐欣^1,3

1. 山东大学口腔医学院种植科,山东济南 250012;
2. 安徽省立医院口腔科,安徽合肥 230001;
3. 山东省口腔组织再生重点实验室,山东济南 250012

收稿日期:2018-01-05 修回日期:2018-07-04 出版日期:2019-02-25 发布日期:2019-04-12
通讯作者: 徐欣, E-mail：xinxu@sdu.edu.cn
作者简介:杨宗澄(1991-),男,硕士研究生,E-mail：576825102@qq.com

Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance

YANG Zong-cheng¹, HUANG Hai-yan², XU Xin^1,3

1.Department of Dental Implant, Stomatological Hospital of Shandong University. Jinan 250012, Shandong Province;
2.Department of Stomatology, Anhui Provincial Hospital. Hefei 230001, Anhui Province;
3.Shandong Provincial Key Laboratory of Oral Tissue Regeneration. Jinan 250012, Shandong Province, China

Received:2018-01-05 Revised:2018-07-04 Online:2019-02-25 Published:2019-04-12

摘要/Abstract

摘要： 目的:探讨长链非编码RNA PCAT-1与口腔鳞状细胞癌发病的相关性。方法:收集2013年6月—2016年6月安徽省立医院口腔科收治的口腔鳞状细胞癌（OSCC）组织标本60例和非癌患者口腔组织标本52例,对OSCC患者标本中PCAT-1水平及其他临床特征（年龄、性别、高危习惯、组织分期、淋巴结转移、分化程度）进行分析;同时用RT-PCR检测标本中PCAT-1和c-Myc的表达水平,并分析其与口腔鳞状细胞癌发病的相关性。采用Graphpad Prism 7.0软件对数据进行统计学分析。结果:对60例口腔鳞状细胞癌标本结合临床信息进行分析,高危习惯（烟酒嗜好）组及淋巴结转移组PCAT-1水平显著高于无高危习惯组及无淋巴结转移组; PCAT-1和c-Myc表达水平显著高于非癌患者口腔组织标本的表达水平,且两者的表达呈正相关。结论:PCAT-1在口腔鳞状细胞癌中显著高表达,可能与口腔癌的发病有关。

关键词: 长链非编码RNA, PCAT-1, c-Myc, 口腔鳞状细胞癌

Abstract: PURPOSE: To investigate the correlation between the expression of lncRNA PCAT-1 and clinicopathological features of oral squamous cell carcinoma. METHODS: In this study, 112 oral tissue specimens, including 60 oral squamous cell carcinoma, and 52 non-cancer oral tissues were collected from Department of Stomatology in Anhui Provincial Hospital during 2013 to 2016. Reverse transcription quantitative PCR (RT-PCR) was performed to detect the expression of PCAT-1 and c-Myc. The correlation between the levels of PCAT-1 and clinical features (age, gender, high risk habit, histological stage, cervical lymphatic metastasis, differentiation) were analyzed. Graphpad Prism 7.0 software package was used for statistical analysis. RESULTS: Significant different levels of PCAT-1 were found between the high risk habit (alcohol and tobacco) group and low risk habit group, between patients with cervical lymphnode metastasis and patients without cervical lymphnode metastasis. The expression of PCAT-1 and c-Myc were up-regulated in OSCC, and there were positive correlation between the expression of PCAT-1 and c-Myc; up-regulated expression of PCAT-1 may be associated with higher morbidity of OSCC. CONCLUSIONS: PCAT-1 is overexpressed in oral squamous cell carcinoma and is associated with higher morbidity of OSCC.

Key words: Long non-coding RNA, PCAT-1, c-Myc, Oral squamous cell carcinoma

中图分类号:

R739.8

杨宗澄, 黄海燕, 徐欣. 长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义[J]. 上海口腔医学, 2019, 28(1): 67-70.

YANG Zong-cheng, HUANG Hai-yan, XU Xin. Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance[J]. Shanghai Journal of Stomatology, 2019, 28(1): 67-70.

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长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义

Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance

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