细胞角蛋白19在口腔鳞状细胞癌中的表达及意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (5): 600-603.

细胞角蛋白19在口腔鳞状细胞癌中的表达及意义

谭丹, 黎春晖, 聂敏海

泸州医学院附属口腔医院口腔内科,四川泸州 646000

收稿日期:2015-12-30 修回日期:2016-06-20 出版日期:2016-10-25 发布日期:2016-11-10
通讯作者: 聂敏海,E-mail:nieminhai@126.com
作者简介:谭丹（1986-）,女,硕士,医师,E-mail:594829202@qq.com

Expression of cytokeratin 19 in the development and progression of oral squamous cell carcinoma

TAN Dan, LI Chun-hui, NIE Min-hai.

Received:2015-12-30 Revised:2016-06-20 Online:2016-10-25 Published:2016-11-10

摘要/Abstract

摘要： 目的研究细胞角蛋白19（cytokeratin 19,CK19）在口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）中的表达,探讨两者之间的相关性。方法收集正常口腔黏膜、单纯上皮增生、上皮异常增生和OSCC患者活检标本49例,采用免疫组织化学、蛋白质印迹比较各组织中CK19的表达;收集未接受过放疗和化疗的OSCC患者16例及健康人群17例的血清,采用ELISA方法检测血清中CK19的可溶性片段CYFRA21-1的水平。采用SPSS17.0软件包进行数据处理。结果CK19表达于有上皮异常增生的复层鳞状上皮基底上层和OSCC尤其是低分化鳞癌的癌细胞中,随上皮异常增生程度加重,CK19表达的阳性率、表达强度显著增加。OSCC患者血清CYFKA21-1含量显著高于健康对照组（P<0.01）。结论CK19表达增加是口腔黏膜癌变过程中的早期事件,其异常表达可作为早期诊断OSCC的辅助指标之一。

关键词: 口腔鳞状细胞癌, 细胞角蛋白19, 癌前状态, CYFRA21-1

Abstract: PURPOSE: To investigate the expression of cytokeratin 19 CK19 in various stages of oral squamous cell carcinoma OSCC, and to explore the relation between CK19 and OSCC. METHODS: Forty-nine specimens including normal oral mucosa, epithelial hyperplasia, epithelial dysplasia, and oral squamous carcinoma were investigated. The expression of CK19 was detected by immunohistochemistry and Western blot. The serum of OSCC patients and healthy people was collected and CYFRA21-1 level was determined by ELISA. SPSS17.0 software package was used for data elevated. RESULTS: CK19 was detectable in suprabasal cell layers in epithelia dysplasia and in oral cancer, especially in poorly-differentiated cancerous cells. With epithelia dysplasia becoming worse, the positive rate and the intensity of CKI9 raised significantly. CYKA21-1 in OSCC was significantly higher than that in normal control groupP< 0.01. CONCLUSIONS: CK19 overexpression is an early event in the process of oral mucosal canceration. Its abnormal expression can be used as one of the reliable indexes of early diagnosis of OSCC.

Key words: Oral squamous cell carcinoma, Cytokeratin 19, Precancerous state, CYFRA21-

中图分类号:

R739.8

谭丹, 黎春晖, 聂敏海. 细胞角蛋白19在口腔鳞状细胞癌中的表达及意义[J]. 上海口腔医学, 2016, 25(5): 600-603.

TAN Dan, LI Chun-hui, NIE Min-hai.. Expression of cytokeratin 19 in the development and progression of oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2016, 25(5): 600-603.

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