纯钛在仿真蛋白环境中腐蚀性能的电化学阻抗谱分析

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (5): 543-546.

纯钛在仿真蛋白环境中腐蚀性能的电化学阻抗谱分析

吕继新¹, 翁维民²

1.浙江横店集团医院口腔科,浙江东阳 322118; 2.上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,上海市口腔医学重点实验室,上海 200011

收稿日期:2014-04-29 修回日期:2014-07-09 出版日期:2014-10-20 发布日期:2015-02-04
通讯作者: 翁维民,Tel:021-23271699,E-mail:wengweimin31@126.com
作者简介:吕继新（1973-）,男,学士,副主任医师,Tel：0579-86586933,E-mail:zjdyljx@126.com
基金资助:
上海市科学技术委员会资助项目(08DZ2271100)

Electrochemical impedance spectroscopic study of titanium in physiological environment with blood-serum protein

LV Ji-xin¹, WENG Wei-min²

1.Department of Stomatology, Hengdian Group Hospital. Dongyong 322118, Zhejiang Province; 2.Department of Prosthodontics, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2014-04-29 Revised:2014-07-09 Online:2014-10-20 Published:2015-02-04
Supported by:
; Supported by Research Fund of Science and Technology Committee of Shanghai Municipality (08DZ2271100)

摘要/Abstract

摘要： 目的在高仿真环境中,采用电化学阻抗法分析纯钛种植材料的腐蚀性能。方法以PBS液为对照组,细胞培养液为实验组,采用电化学分析仪测试钛在含血清蛋白体系中的电化学阻抗谱,利用EIS-Nyquist图、EIS-Bode图及等效电路的拟合分析,研究血清蛋白对钛的抗腐蚀性能的影响,采用SPSS11.0软件包对数据进行统计学分析。结果纯钛材料在细胞培养液中的氧化膜阻抗值（24.8±3.9）×10⁵ Ω显著大于在PBS液中的阻抗值（6.7±0.8）×10⁵ Ω（P<0.05）。纯钛在PBS中低频区的相位角约为55°,而在细胞培养液中低频区的相位角约为75°。结论机体环境中的血清蛋白能够影响钛金属在体液中的腐蚀性能。

关键词: 钛, 腐蚀, 电化学阻抗, 血清蛋白

Abstract: PURPOSE: Electrochemical impedance spectroscopy (EIS) was employed to study the corrosion behaviour of titanium in dummy environment with blood-serum protein. METHODS: EIS of titanium in PBS and cell culture fluid was acquired by advanced electrochemical system. The characteristic of EIS-Nyquist and EIS-Bode were investigated and the equivalent circuit was used to analyze the EIS results. The data was analyzed using SPSS 11.0 software package. RESULTS: The corrosion resistance of the titanium in cell culture fluid was significantly higher than that in PBS (P<0.05). The phase angle of TiO₂-based passive film at low frequency decreased to 55° when titanium was in PBS, but 75° when in cell culture fluid. CONCLUSIONS: The blood-serum protein plays an important role in the corrosion behaviour of titanium in physiological environment.

Key words: Titanium, Corrosion, Electrochemical impedance spectroscopy, Blood-serum protein

吕继新, 翁维民. 纯钛在仿真蛋白环境中腐蚀性能的电化学阻抗谱分析[J]. 上海口腔医学, 2014, 23(5): 543-546.

LV Ji-xin, WENG Wei-min. Electrochemical impedance spectroscopic study of titanium in physiological environment with blood-serum protein[J]. Shanghai Journal of Stomatology, 2014, 23(5): 543-546.

参考文献

[1] Yu WQ, Qiu J, Xu L, et al. Corrosion behavior of TiO2 nanotube layers on titanium in Hank’s solution[J]. Biomed Mater,2009,4(6): 065012.
[2] 孙桂兰, 陈建治, 王磊, 等. 纯钛表面微弧氧化膜的耐腐蚀性研究 [J]. 中华口腔医学杂志, 2007, 42(8): 501-503.
[3] Cheng X, Roscoe SG. Corrosion behavior of titanium in the presence of calcium phosphate and serum proteins [J]. Biomaterials, 2005, 26(35): 7350-7356.
[4] Yu WQ, Qiu J, Zhang FQ. In vitro corrosion study of different TiO2 nanotube layers on titanium in solution with serum proteins[J]. Colloids Surf B Biointerfaces, 2011, 84(2): 400-405.
[5] 曹楚南, 张鉴清. 电化学阻抗谱导论[M]. 北京：科学出版社, 2002: 20-24.
[6] 张智, 熊金平, 曹京宜, 等. 不同表面处理等级下有机涂层失效行为的EIS研究 [J].新技术新工艺·热加工工艺技术与材料研究, 2008, 28(10): 90-93.
[7] Rodrigues DC, Urban RM, Jacobs JJ, et al. In vivo severe corrosion and hydrogen embrittlement of retrieved modular body titanium alloy hip-implants [J]. J Biomed Mater Res B Appl Biomater, 2009, 88(1): 206-219.
[8] Aparicio C, Gil FJ, Fonseca C, et al. Corrosion behaviour of commercially pure titanium shot blasted with different materials and sizes of shot particles for dental implant applications [J]. Biomaterials, 2003, 24(2): 263-273.
[9] Karpagavalli R, Zhou A, Chellamuthu P, et al. Corrosion behavior and biocompatibility of nanostructured TiO 2 film on Ti6Al4V [J]. J Biomed Mater Res A, 2007, 83(4): 1087-1095.
[10] Takemoto S, Hattori M, Yoshinari M, et al. Corrosion behavior and surface characterization of titanium in solution containing fluoride and albumin [J]. Biomaterials, 2005, 26(8): 829-837.
[11] 曹轶婷, 于卫强, 翁维民. 含牛血清蛋白人工唾液对纯钛腐蚀性能的影响研究 [J]. 上海交通大学学报(医学版), 2012, 32(3): 293-295.
[12] Rondelli G, Torricelli P, Fini M, et al. In vitro corrosion study by EIS of a nickel-free stainless steel for orthopaedic applications [J]. Biomaterials, 2005, 26(7): 739-744.
[13] Bozzini B, Carlino P, D’Urzo L, et al. An electrochemical impedance investigation of the behaviour of anodically oxidised titanium in human plasma and cognate fluids, relevant to dental applications [J]. J Mater Sci Mater Med, 2008, 19(11): 3443-3453.
[14] 曹京宜, 熊金平, 李水冰, 等. 利用EIS高频区参数评价2种环氧涂层的性能[J]. 化工学报, 2008, 59(11): 2851-2856.

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