3D打印个体化钛网的机械力学性能及生物相容性分析

doi:10.19439/j.sjos.2020.03.005

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (3): 250-256.doi: 10.19439/j.sjos.2020.03.005

3D打印个体化钛网的机械力学性能及生物相容性分析

张耀升^1,*, 张锴^2,*, 陈欣慰³, 牟海彰¹, 丁旺旺⁴, 秦明礼⁴, 张善勇^3#, 龚勤林^2#, 陈刚^4#, 徐伟峰³, 虞科恩², 姜艳梅²

1.潍坊医学院口腔医学院,山东潍坊 261053;
2.四川大学,四川成都 610065;
3.上海交通大学医学院附属第九人民医院·口腔医学院口腔外科,上海口腔医学重点实验室,上海 200011;
4.北京科技大学新材料技术研究院,北京 100083

收稿日期:2019-11-28 修回日期:2020-01-10 出版日期:2020-06-25 发布日期:2020-07-29
通讯作者: 张善勇,E-mail:zhangshanyong@126.com;龚勤林,E-mail: gongqinlin@scu.edu.cn;陈刚,E-mail:gche098@ustb.edu.cn。^#共同通信作者
作者简介:张耀升（1993-）,女,在读硕士研究生,E-mail:839412743@qq.com;张锴（1998-）,男,在读本科生,E-mail:1446959546@qq.com。*并列第一作者
基金资助:
2016年浦东新区卫计委联合攻关项目（PW2016D-1）; 四川大学大学生创新创业训练计划项目（T2020109750）; 上海市卫生和计划生育委员会面上项目（M20170232)

Mechanical properties of 3D-printed titanium mesh and its biocompatibility in vitro

ZHANG Yao-sheng¹, ZHANG Kai², CHEN Xin-wei³, MU Hai-zhang¹, Ding Wang-wang⁴, Qin Ming-li⁴, ZHANG Shan-yong³, GONG Qin-lin², Chen Gang⁴, XU Wei-feng³, YU Ke-en², JIANG Yan-mei²

1. College of Stomatology, Weifang Medical University. Weifang 261053, Shandong Province;
2. Sichuan University. Chengdu 610065, Sichuan Province;
3. Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
4. University of Science and Technology Beijing. Beijing 100083, China

Received:2019-11-28 Revised:2020-01-10 Online:2020-06-25 Published:2020-07-29

摘要/Abstract

摘要： 目的比较3D打印钛网与成品钛网的机械力学性能差异,并评价3D打印钛网对细胞生长及分化的影响。方法采用激光打印技术制备个体化3D打印钛网,用静态拉伸压缩载荷实验检测机械力学性能,评估机械力学性能。选择4周龄雄性SD大鼠骨髓间充质干细胞,与不同孔径大小的3D打印钛网共培养。采用CCK-8法检测各组细胞增殖情况;对各组细胞进行成骨诱导分化后,检测碱性磷酸酶（ALP）活性改变,运用实时荧光定量PCR检测相关成骨基因的表达;通过扫描电镜及活/死细胞染色,观察细胞在3D打印钛网上的黏附和生长情况。采用SPSS 22.0软件包对数据进行统计学分析。结果 3D打印钛网的拉伸压缩负载实验结果显示其具有优异的力学性能;相比对照组,不同孔径钛网对细胞增殖的影响不大,细胞在钛网上显现出良好的黏附和生长状态。3D打印钛网对成骨分化具有一定的促进作用。结论 3D打印钛网的机械力学性能优异,且具有良好的生物相容性。

关键词: 3D打印钛网, 机械力学性能, 生物相容性

Abstract: PURPOSE: To compare the mechanical properties of 3D-printed titanium meshes and pre-shaped titanium meshes, and to evaluate the effects of 3D-printed titanium meshes on cell proliferation and differentiation. METHODS: 3D- printed titanium meshes were produced and prepared with laser printing machine. The mechanical properties were analyzed by static tension and compression load test. Bone marrow mesenchymal stem cells (BMSCs) were extracted from 4-week-old male SD rats. BMSCs were co-cultured with 3D-printed titanium meshes of different apertures. Cell counting kit-8 (CCK-8) assay was used to detect cell proliferation. Alkaline phosphatase (ALP) activity assay was used to test ALP activity. The expression of related osteogenic genes was tested by real-time PCR. The adhesion and growth of BMSCs were investigated by scanning electron microscopy (SEM) and living / dead cell staining. SPSS 22.0 software package was used for statistical analysis of the results. RESULTS: The results of 3D-printing Ti-meshes tension and compression loading experiment were excellent. The 3D-printing Ti-meshes showed no inhibitory effects on cell proliferation, survival and adhesion, but had a positive effect on osteogenesis of BMSCs. CONCLUSIONS: The mechanical properties of 3D-printed Ti-meshes are excellent. The 3D-printed Ti-meshes have good biocompatibility.

Key words: 3D-Printing titanium mesh, Mechanical Properties, Biocompatibility

中图分类号:

R782.1

张耀升, 张锴, 陈欣慰, 牟海彰, 丁旺旺, 秦明礼, 张善勇, 龚勤林, 陈刚, 徐伟峰, 虞科恩, 姜艳梅. 3D打印个体化钛网的机械力学性能及生物相容性分析[J]. 上海口腔医学, 2020, 29(3): 250-256.

ZHANG Yao-sheng, ZHANG Kai, CHEN Xin-wei, MU Hai-zhang, Ding Wang-wang, Qin Ming-li, ZHANG Shan-yong, GONG Qin-lin, Chen Gang, XU Wei-feng, YU Ke-en, JIANG Yan-mei. Mechanical properties of 3D-printed titanium mesh and its biocompatibility in vitro[J]. Shanghai Journal of Stomatology, 2020, 29(3): 250-256.

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3D打印个体化钛网的机械力学性能及生物相容性分析

Mechanical properties of 3D-printed titanium mesh and its biocompatibility in vitro

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