上海口腔医学

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (4): 385-390.doi: 10.19439/j.sjos.2023.04.009

• 论著 • 上一篇    下一篇

口腔鳞癌组织中RACK1与EGP40的表达及其与临床病理特征及预后的相关性

段咏华1, 吴开柳2, 梅健1, 周谊渊1, 刘梦石1   

  1. 1.上饶市人民医院 口腔科,江西 上饶 334000;
    2.上海交通大学医学院附属第九人民医院 口腔颌面-头颈肿瘤科,上海 200011
  • 收稿日期:2022-07-25 修回日期:2022-09-20 发布日期:2023-09-07
  • 通讯作者: 段咏华,E-mail：dhy1347937@163.com
  • 作者简介:段咏华（1976-）,女,硕士, 副主任医师
  • 基金资助:
    江西省自然科学基金（2019864）

Expression of RACK1 and EGP40 in oral squamous cell carcinoma and its correlation with clinicopathological features and prognosis

DUAN Yong-hua1, WU Kai-liu2, MEI Jian1, ZHOU Yi-yuan1, LIU Meng-shi1   

  1. 1. Department of Stomatology, Shangrao People's Hospital. Shangrao 334000, Jiangxi Province;
    2. Deparment of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine. Shanghai 200011, China
  • Received:2022-07-25 Revised:2022-09-20 Published:2023-09-07

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摘要： 目的 探讨口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）癌组织中活性蛋白激酶C受体1（activated protein kinase C receptor 1,RACK1）与上皮糖蛋白40（epithelin glycoprotein 40,EGP40）的表达及其与临床病理特征及预后的关系。方法 选择2016年1月—2019年2月上饶市人民医院收治的103例OSCC患者作为研究对象,均行OSCC根治术,术后随访3年。采用免疫组织化学法检测癌组织、癌旁组织中RACK1与EGP40的表达水平。对比癌组织、癌旁组织中RACK1与EGP40的阳性表达情况,分析OSCC患者癌组织中RACK1与EGP40阳性表达水平与临床病理参数的关系,分析影响OSCC患者术后复发、转移的因素,癌组织中RACK1与EGP40的表达与术后无瘤生存的关系。采用SPSS 18.0软件包对数据进行统计学分析。结果 癌组织中RACK1与EGP40阳性表达率显著高于癌旁组织（P<0.05）。低分化、临床Ⅲ期、有颈淋巴结转移、有浸润脉管的OSCC患者癌组织中RACK1与EGP40阳性表达率分别高于中高分化、临床Ⅱ期、无颈淋巴结转移、无浸润脉管者（P<0.05）,T3期OSCC患者癌组织中RACK1阳性表达率显著高于T2期（P<0.05）。Cox多因素回归分析显示,病理分期（RR=6.290,95%CI：2.588~15.287）、颈淋巴结转移（RR=5.995,95%CI：2.467~14.571）、RACK1阳性（RR=4.495,95%CI：1.850~10.925）、EGP40阳性（RR=4.559,95%CI：1.876~11.079）是影响OSCC患者术后复发、转移的因素（P<0.05）。RACK1阴性表达患者的无瘤生存曲线优于阳性表达患者（P<0.05）,EGP40阴性表达患者的无瘤生存曲线优于阳性表达患者（P<0.05）。结论 OSCC患者癌组织中RACK1与EGP40的表达与临床病理参数及预后相关,RACK1、EGP40阳性表达患者术后复发、转移风险增加。

关键词: 口腔鳞癌, 活性蛋白激酶C受体1, 上皮糖蛋白40, 病理特征, 预后

Abstract: PURPOSE: To investigate the expression of tissue-active protein kinase C receptor 1 (RACK1) and epithelin glycoprotein 40 (EGP40) in oral squamous cell carcinoma (OSCC) and their relationship with clinicopathological features and prognosis. METHODS: A total of 103 patients with OSCC who were admitted to Shangrao People's Hospital from January 2016 to February 2019 were prospectively selected as the research subjects. All patients underwent radical resection of OSCC and were followed up for 3 years. Immunohistochemistry was used to detect the positive expression levels of RACK1 and EGP40 in cancer tissues and adjacent tissues. The positive expression of RACK1 and EGP40 in cancer tissues and adjacent tissues were compared. The relationship between the positive expression level of RACK1 and EGP40 in cancer tissues of OSCC patients and clinicopathological parameters was analyzed. Factors affecting postoperative recurrence and metastasis in OSCC patients were analyzed. The relationship between the expression of RACK1 and EGP40 in cancer tissues and postoperative disease-free survival of OSCC patients was analyzed. SPSS 18.0 software package was used for statistical analysis of the data. RESULTS: The positive expression rate of RACK1 and EGP40 in cancer tissues was significantly higher than those in adjacent tissues (P<0.05). The positive expression rate of RACK1 and EGP40 in cancer tissues of OSCC patients with poorly differentiated, stage III, cervical lymph node metastasis, and infiltrating vessels was significantly higher than that in patients with moderate and high differentiation, stage II, no cervical lymph node metastasis, and no infiltrating vessels(P<0.05). The positive expression rate of RACK1 in cancer tissue of OSCC patients in T3 stage was significantly higher than that in T2 stage(P<0.05). Cox multivariate regression analysis showed pathological grade (RR=6.290, 95%CI: 2.588-15.287), cervical lymph node metastasis(RR=5.995, 95%CI: 2.467-14.571), RACK1 positive rate (RR=4.495, 95%CI: 1.850-10.925) and EGP40 positive rate (RR=4.559, 95%CI: 1.876-11.079) were factors affecting the recurrence and metastasis of OSCC patients after surgery(P<0.05). The disease-free survival curve of patients with negative expression of RACK1 was significantly better than that of patients with positive expression (P<0.05). The disease-free survival curve of patients with negative expression of EGP40 was significantly better than that of patients with positive expression (P<0.05). CONCLUSIONS: The expression of RACK1 and EGP40 in cancer tissues of OSCC patients is related to clinicopathological parameters and prognosis. Patients with positive expression of RACK1 and EGP40 have a high risk of recurrence and metastasis after surgery.

Key words: Oral squamous cell carcinoma, RACK1, EGP40, Pathological features, Prognosis

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