miR-199b-5p在头颈癌细胞中的表达及对细胞增殖和侵袭能力的影响

doi:10.19439/j.sjos.2018.03.010

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (3): 265-269.doi: 10.19439/j.sjos.2018.03.010

miR-199b-5p在头颈癌细胞中的表达及对细胞增殖和侵袭能力的影响

王茜，李光辉，郭嘉，孙强

郑州大学第一附属医院口腔医学中心，河南郑州 450052

收稿日期:2017-03-06 修回日期:2017-08-10 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: 孙强，E-mail：franklin1082@163.com
作者简介:王茜（1983-），女，博士，主治医师，E-mail：sissy9288@163.com
基金资助:
国家自然科学基金（31400839，81402231）；河南省科技厅基础研究和前沿技术研究项目（142300410315）；郑州市口腔颌面外科院士工作站资助项目（152PYSGZ040）

miR-199b-5p inhibits proliferation and invasion of head and neck cancer cells

WANG Xi, LI Guang-hui, GUO Jia, SUN Qiang.

Department of Stomatology, the First Affiliated Hospital of Zhengzhou University. Zhengzhou 450052, Henan Province, China

Received:2017-03-06 Revised:2017-08-10 Online:2018-07-20 Published:2018-07-20

摘要/Abstract

摘要： 目的： 明确miR-199b-5p差异表达在头颈部鳞状细胞癌（HNSCC）发生、发展中的作用并探讨其生物学功能。方法： 检测128例HNSCC组织中miR-199b-5p的表达，通过Kaplan-Meier生存分析，分析临床病理参数与miR-199b-5p表达水平之间的关系。通过miRNA异常表达对HNSCC细胞生长、侵袭和迁移能力以及克隆形成的影响，检测HNSCC细胞中miRNA的功能。采用SPSSl9.0软件包对数据进行t检验。结果： 在79例淋巴结转移的HNSCC组织中，miR-199b-5p的表达水平为1.68±0.21；在49例非淋巴结转移的HNSCC组织中，其表达水平为2.64±0.24，差异显著（P=0.001）。在HNSCC患者中，高表达miR-199b-5p的患者总体存活率显著增高（P=0.01），且高表达水平的miR-199b-5p可以显著抑制头颈鳞癌细胞的侵袭和转移。结论： 作为抑癌基因，miR-199b-5p在HNSCC的发生、发展中起着关键作用。

关键词: miR-199b-5p, 头颈鳞癌, 转移, 侵袭

Abstract: PURPOSE: This study identified the differentially expressed miR-199b-5p in the progressing of head and neck squamous cell carcinoma(HNSCC) and investigated its biological characters. METHODS: The expression of miR-199b-5p in 128 HNSCC tissues samples was evaluated. The association between clinicopathological parameters and the expression levels of the candidated miRNAs was analyzed by using Kaplan-Meier survival analysis. Cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells. Statistical analysis was performed using SPSS 19.0 software package. RESULTS: In 79 HNSCC tissues with cervical lymph node metastasis, the expression level of miR-199b-5p was 1.68±0.21; while the expression level was 2.64±0.24 in 28 tissues without lymph node metastasis (P=0.001). In patients with HNSCC, lower level of miR-199b-5p expression significantly correlated with worse overall survival rate (P=0.01). Overexpression of miR-199b-5p inhibited HNSCC cell proliferation and invasion. CONCLUSIONS: miR-199b-5p plays a key role in cell invasion and metastasis and its expression correlated with overall survival in patients with HNSCC.

Key words: miR-199b-5p, HNSCC, Metastasis, Invasion

中图分类号:

R739.8

王茜，李光辉，郭嘉，孙强. miR-199b-5p在头颈癌细胞中的表达及对细胞增殖和侵袭能力的影响[J]. 上海口腔医学, 2018, 27(3): 265-269.

WANG Xi, LI Guang-hui, GUO Jia, SUN Qiang.. miR-199b-5p inhibits proliferation and invasion of head and neck cancer cells[J]. Shanghai Journal of Stomatology, 2018, 27(3): 265-269.

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miR-199b-5p在头颈癌细胞中的表达及对细胞增殖和侵袭能力的影响

miR-199b-5p inhibits proliferation and invasion of head and neck cancer cells

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