白杨黄素对成骨细胞系MC3T3-E1成骨向分化能力的影响

doi:10.19439/j.sjos.2018.03.009

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (3): 261-264.doi: 10.19439/j.sjos.2018.03.009

白杨黄素对成骨细胞系MC3T3-E1成骨向分化能力的影响

夏冰，洪滔，何欣

杭州市红十字会医院口腔科，浙江杭州 310000

收稿日期:2018-02-11 修回日期:2018-03-23 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: 洪滔，E-mail:htown@163.com
作者简介:夏冰（1982-），男，硕士，主治医师，E-mail: 18911031@qq.com

Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells

XIA Bing, HONG Tao, HE Xin.

Department of Stomatology, Hangzhou Red Cross Hospital. Hangzhou 310000, Zhejiang Province, China

Received:2018-02-11 Revised:2018-03-23 Online:2018-07-20 Published:2018-07-20

摘要/Abstract

摘要： 目的： 探讨黄酮类小分子白杨黄素对成骨细胞系MC3T3-E1成骨向分化能力的影响。方法： 检测白杨黄素的细胞毒性后，确定最佳作用浓度。采用DMEM完全培养基将成骨细胞系MC3T3-E1制成单细胞悬液，加入浓度为25 μmol/L的白杨黄素作为实验组，以单纯DMEM组作为阴性对照组，以成骨诱导液组（普通DMEM培养基中加入10 mmol/L β甘油磷酸钠、50 μmol/L维生素C和10 nmol/L地塞米松）作为阳性对照组。体外培养后，运用实时定量PCR分别检测早、中、晚期成骨相关指标Runx2、ColA1和OCN mRNA的表达。采用SPSS17.0软件包对数据进行统计学分析。结果： 浓度为25 μmol/L的白杨黄素对MC3T3-E1细胞培养无毒性；在添加25 μmol/L的白杨黄素共培养7 d后，Runx2的mRNA表达量达到峰值， 14 d后ColA1的mRNA表达量达到峰值；共培养21 d后，OCN的mRNA表达量达到峰值。结论： 25 μmol/L的黄酮类小分子白杨黄素能够促进成骨细胞系MC3T3-E1成骨分化过程中成骨相关基因的表达。

关键词: 白杨黄素, 成骨分化, MC3T3-E1

Abstract: PURPOSE: To investigation the effect of flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells. METHODS: After evaluation of the toxic effect of chrysin, preosteoblast MC3T3-E1 cell suspension was prepared with DMEM and then the cells were cultured with 0 and 25 μmol/L chrysin. Real-time quantitative PCR was used to detect the expression of osteogenic marlcers including Runx2, ColA1 and OCN. Statistical analysis was performed using SPSS17.0 software package. RESULTS: 25 μmol/L chrysin had no toxic effect on preosteoblast MC3T3-E1 cells. After co-culture with 25 μmol/L chrysin,the expression of Runx2, ColA1 and OCN was highest at the 7th,14th and 21th day,respectively.CONCLUSIONS: : 25 μmol/L chrysin can promote osteogenic gene expression of preosteoblast MC3T3-E1 cell.

Key words: Chrysin, Osteogenic differentiation, MC3T3-E1

中图分类号:

R381.01

夏冰，洪滔，何欣. 白杨黄素对成骨细胞系MC3T3-E1成骨向分化能力的影响[J]. 上海口腔医学, 2018, 27(3): 261-264.

XIA Bing, HONG Tao, HE Xin.. Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells[J]. Shanghai Journal of Stomatology, 2018, 27(3): 261-264.

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白杨黄素对成骨细胞系MC3T3-E1成骨向分化能力的影响

Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells

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