PLGA/鱼皮胶原共轭静电纺丝膜生物相容性分析

doi:10.19439/j.sjos.2018.03.005

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (3): 244-247.doi: 10.19439/j.sjos.2018.03.005

PLGA/鱼皮胶原共轭静电纺丝膜生物相容性分析

陆小丽，缪惠灵，柳锋，高美琴

南通市口腔医院，江苏南通 226006

收稿日期:2018-04-08 修回日期:2018-05-11 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: 高美琴，E-mail:ntgmq@126.com
作者简介:陆小丽(1984-)，女，在职博士研究生，主治医师，E-mail:284315760@qq.com
基金资助:
南通市科技项目（MS12016019）

Analysis of the biocompatibility of a new PLGA/fish skin collagen conjugated electrostatic spinning film

LU Xiao-li, MIAO Hui-ling, LIU Feng, GAO Mei-qin

Nantong Stomatological Hospital. Nantong 226006, Jiangsu Province, China

Received:2018-04-08 Revised:2018-05-11 Online:2018-07-20 Published:2018-07-20

摘要/Abstract

摘要： 目的： 对一种新型聚乳酸-羟基乙酸（polylactic acid-glycolic acid，PLGA）/鱼皮胶原共轭静电纺丝膜的生物相容性进行分析。方法： 以PLGA和医用级鱼皮Ⅰ型胶原为原材料，通过共轭静电纺丝技术制备出高取向性的纳米纤维膜，然后分别以小鼠成纤维细胞L929为研究模型，初步评价其细胞、组织相容性，为其用于种植骨组织再生（guided bone regeneration，GBR）提供实验依据。结果： 纤维细胞在纤维膜表面黏附生长良好，有大量细胞伸出伪足，沿着纤维的取向进行铺展排列。结论： PLGA/鱼皮胶原共轭静电纺丝膜有利于成纤维细胞生长，可望用于种植骨组织再生。

关键词: 共轭静电纺丝技术, 纳米纤维膜, 生物相容性, 引导骨组织再生

Abstract: PURPOSE: To study the biocompatibility of a new polylactic acid-glycolic acid(PLGA)/fish skin collagen conjugated electrostatic spinning film. METHODS: PLGA and medical-grade fish skin-I collagen were used as raw materials to prepare nanometer fiber films by conjugated electrostatic spinning technique. Mouse fibroblast L929 was used as a model to evaluate its cell and histocompatibility, which provided experimental basis for application in guided bone regeneration (GBR). RESULTS: Fibroblasts showed good adhesion and growth on the surface of fiber membrane; there were a large number of deep pseudopodias, which were arranged along the orientation of fibers. CONCLUSIONS: PLGA/fish skin collagen conjugated electrostatic spinning membrane is beneficial to fibroblast growth and is expected to be used for GBR.

Key words: Conjugated electrostatic spinning technology, Nanofilms, Biocompatibility, Guided bone regeneration

中图分类号:

R783.1

陆小丽，缪惠灵，柳锋，高美琴. PLGA/鱼皮胶原共轭静电纺丝膜生物相容性分析[J]. 上海口腔医学, 2018, 27(3): 244-247.

LU Xiao-li, MIAO Hui-ling, LIU Feng, GAO Mei-qin. Analysis of the biocompatibility of a new PLGA/fish skin collagen conjugated electrostatic spinning film[J]. Shanghai Journal of Stomatology, 2018, 27(3): 244-247.

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PLGA/鱼皮胶原共轭静电纺丝膜生物相容性分析

Analysis of the biocompatibility of a new PLGA/fish skin collagen conjugated electrostatic spinning film

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