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Study on the effect of alginate impression spray disinfection process on model accuracy ZHANG Jia-yuan, YU De-dong, YE Li-juan, WU Chun-lan, LIU Jing Shanghai Journal of Stomatology    2023, 32 (5): 449-454.   DOI: 10.19439/j.sjos.2023.05.001 Abstract （142）      PDF （1125KB）（156）       Knowledge map    PURPOSE: To investigate the effect of a new cleaning and disinfection process of oral impressions on the accuracy of alginate dental impression. METHODS: Sixteen young volunteers were selected to make alginate oral impression (2 times for each upper and lower jaw) and oral scandata acquisition (1 time for each upper and lower jaw) to obtain STL data. The 2 pairs of alginate impressions of each subject were numbered group 1, group 2, inwhich group 1 was not sterilized and the oral impression film in group 2 was treated with the method and steps of new oral impression cleaning and disinfection. All the oral impressions of the subjects were perfused with plaster models in the same standard way and demoulded. The obtained plaster models were examined and scanned after appropriate trimming and grinding. The plaster model data obtained from each group of each patient after warehouse scanning were compared with the orally scanned model data for alignment analysis, and root mean square(RMS) was used as a parameter to evaluate the deviation between the test model and the standard model. The chromatograms of the deviations were saved for visualization and analysis to observe the differences in model accuracy from several dimensions. Statistical analysis was performed with SPSS 23.0 software package. RESULTS: The differences in RMS of model accuracy of maxillary dental series of alginate impressions were statistically significant, in which the RMS of the group disinfecting the maxillary oral impression film with a new oral impression cleaning and disinfection process was significantly higher than that of the nonsterilized group (P=0.006), while the difference between model accuracy RMS of the mandibular disinfection group and non-disinfection group was not statistically significant(P=0.874). CONCLUSIONS: The new technical specifications for cleaning and disinfection of dental impressions recommended by the Chinese Stomatological Association have certain effects on the accuracy of dental impressions made of alginate material,and the details in its process should be further improved. Reference | Related Articles | Metrics

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Comparison of clear aligners and customized lingual appliance for bimaxillary dentoalveolar protrusion: a retrospective study LI Zhen-xia, YUAN Ling-jun, ZHENG Xiao-wen, LIU Lu, LIU Chao, FANG Bing Shanghai Journal of Stomatology    2023, 32 (5): 480-484.   DOI: 10.19439/j.sjos.2023.05.006 Abstract （98）      PDF （629KB）（154）       Knowledge map    PURPOSE: To compare the treatment effects of clear aligners and customized lingual appliance on treating bimaxillary dentoalveolar protrusion patients with first premolar extractions. METHODS: Fifty-four patients with bimaxillary dentoalveolar protrusion treated in Shanghai Ninth People Hospital were involved in the retrospective study. Twenty-five cases used clear aligners and 29 cases used customized lingual appliance. All of them were treated by extracting 4 first premolars and retracting anterior teeth with strong anchorage. The changes of anterior tooth and soft tissue adduction before and after treatment were compared by lateral cephalometric measurements. SPSS 26.0 software package was used for data analysis. RESULTS: The total course of treatment in the clear aligners group (46.32±7.37 months) was about 10.8 months longer than that in the customized lingual appliance group (35.55±5.90 months) (P<0.05). There was no significant difference in upper incisor retraction, lower incisor inclination and overjet reduction between the two groups(P>0.05). There were significant differences in upper lip retraction, lower lip retraction, upper incisor torque reduction, and overbite reduction between the two groups(P<0.05). Customized lingual appliance group showed a significant improvement of lips retraction and overbite reduction in orthodontic treatment. For the correction of overjet, there was no significant difference between the two groups (P=0.337). The data of U1-OP (the distance between edge of the upper central incisor and the functional occlusal plane) was not in normal distribution, and there was no significant difference between the two groups(P=0.184). CONCLUSIONS: The two techniques can both retract the anterior teeth and lips to improve the profile. However, the customized lingual appliance was more effective in improving the soft tissue profile of patients with bimaxillary dentoalveolar protrusion, with shorter treatment course. Reference | Related Articles | Metrics

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A case of two-stage orthodontic treatment of skeletal ClassⅡmalocclusion with mandibular retrusion and maxillary dental arch stenosis ZHANG Yun, LI Xiao-bing Shanghai Journal of Stomatology    2023, 32 (5): 555-560.   DOI: 10.19439/j.sjos.2023.05.020 Abstract （173）      PDF （2656KB）（143）       Knowledge map    This article reviewed two-stage orthodontic treatment of a patient with skeletal ClassⅡmalocclusion with mandibular retrusion and maxillary dental arch stenosis. The patient was also accompanied by premature loss of bilateral mandibular deciduous canines and mesialization of mandibular posterior teeth. The first-stage treatment mainly corrected the maxillary dental arch stenosis, maxillary anterior teeth protrusion, mandibular retrusion and skeletal ClassⅡmalocclusion, while the second-stage treatment mainly corrected the mesialization of mandibular posterior teeth and alignment of maxillary and mandibular dentition. The two-stage orthodontic treatment finally enabled the patient to achieve good dental arch shape, tooth alignment, skeletal face pattern and soft tissue profile. Reference | Related Articles | Metrics

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Evaluation of red and white aesthetic effects of porcelain veneers in the restoration of developmental anterior dental gaps DENG Xiao-lin, YUAN Shuang, ZHU Dan-ting, SHI Le Shanghai Journal of Stomatology    2023, 32 (3): 276-279.   DOI: 10.19439/j.sjos.2023.03.011 Abstract （147）      PDF （873KB）（140）       Knowledge map    PURPOSE: To evaluate the red and white aesthetic effect of porcelain veneer in the restoration of developmental anterior interdental spaces. METHODS: A total of 152 anterior teeth in 64 patients with developmental anterior dental gaps were restored using porcelain veneers, the aesthetic effects before and after restoration were evaluated by pink aesthetic index (PES) and white aesthetic index(WES), the aesthetic effect of gingival papilla filling and reconstruction was evaluated by interdental gingival papilla index (PIS), and visual analogue score (VAS) was used to compare the satisfaction of patients before and after restoration. SPSS 203.0 software package was used for statistical analysis. RESULTS: The overall mean scores of PES before and after restoration of 152 developmental anterior interdental teeth were 9.63±2.23 and 13.64±0.88, respectively. The average scores of WES before and after restoration were 6.85±1.87 and 9.81±0.58, respectively. There were significant differences of PES and WES scores before and after restoration(P<0.01). According to the requirements of "near" perfect restoration(PES≥13 points, WES≥9 points), the red and white aesthetic effect after restoration was near the standard. The scores of PIS before and after restoration were 1.86±0.67 and 2.97±0.18, the interdental gingival papilla was completely filled with space, and the shape was ideal, there were significant statistical differences before and after restoration(P<0.01). Patients were more satisfied with the smile curve and morphology of the restored anterior teeth than other parameters. CONCLUSIONS: The aesthetic effect of using porcelain veneers to repair developmental anterior interdental gaps is ideal, among which the filling of the papillae between the teeth can meet the expectations of patients, and the aesthetic effect evaluation of PIS and PES/WES applied to porcelain veneers to repair developmental anterior interdental spaces has practical guiding significance in clinical practice. Reference | Related Articles | Metrics

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Morphological changes of upper airway in patients with skeletal Class Ⅲ malocclusion after bimaxillary surgery and correlation analysis YAN Jie-ya, LIN Yi-jun, LI Ya-xin, LAO An, LIAO Sheng-hsuan, LIU Jia-qiang Shanghai Journal of Stomatology    2023, 32 (5): 485-490.   DOI: 10.19439/j.sjos.2023.05.007 Abstract （110）      PDF （952KB）（139）       Knowledge map    PURPOSE: To analyze the morphological changes of the upper airway and related influencing factors in patients with skeletal Class Ⅲ malocclusion after bimaxillary surgery. METHODS: Twenty skeletal Class Ⅲ patients who underwent Le Fort I osteotomy and bilateral sagittal split ramus osteotomy(BSSRO) for maxillary advancement and mandibular setback were selected. The patients received CT scans before(T0) and 3-6 months after surgery, and the images were reconstructed three-dimensionally with Dolphin Imaging 11.9 software. Changes in the volume, cross-sectional area, and landmarks of each soft and hard tissue of the airway were measured. Statistical analysis of the data was performed using SPSS 25.0 software package. RESULTS: The volume of nasopharyngeal airway increased after operation (P<0.05), and the volume of oropharyngeal airway decreased significantly(P<0.01). The cross-sectional area of the airway at the plane of the second cervical vertebra was significantly decreased (P<0.01), and the coronal and sagittal diameters were decreased(P<0.05). The change of nasopharyngeal airway volume was moderately positively correlated with the sagittal change of the posterior nasal spine (r=0.460, P<0.05), and the change of oropharynx and laryngopharyngeal airway volume was positively correlated with the vertical change of the midpoint of the soft palate(r=0.496, 0.696, P<0.05). The airway cross-sectional area in the second and third cervical vertebra planes and the sagittal diameter of the airway in the third cervical vertebra plane were positively correlated with the vertical changes of the midpoint of the soft palate(r=0.474, 0.629, 0.547, P<0.05). The change of airway cross-sectional area at the third cervical vertebra plane was moderately negatively correlated with the change of mandibular plane angle(r=-0.536, P<0.05). CONCLUSIONS: The volume and cross-sectional area of oropharyngeal airway in skeletal Class Ⅲ patients after bimaxillary surgery will decrease. However, the total upper airway volume doesn't change significantly. The changes in the upper airway are correlated with the changes in some soft and hard tissue landmarks. Reference | Related Articles | Metrics

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Study on promoting bone formation in osteoporotic zebrafish by lithium-doped hydroxyapatite nanowires LIU Zhen, GUO Wei-ming, LIU Lu, CHEN Rong-jing, FANG Bing Shanghai Journal of Stomatology    2023, 32 (5): 455-461.   DOI: 10.19439/j.sjos.2023.05.002 Abstract （97）      PDF （2023KB）（134）       Knowledge map    PURPOSE: To observe the regulatory effect of lithium-doped hydroxyapatite nanowires on bone metabolism in osteoporotic zebrafish induced by dexamethasone. METHODS: Pure hydroxyapatite nanowires(nHA) and hydroxyapatite nanowires doped with 10% lithium ions (Li-nHA) were prepared by using hydrothermal method, and then material characterization was performed. The juvenile zebrafish cultured for 3 days(3dpf) were selected and co-cultured with nHA and Li-nHA extracts up to 7dpf. A negative(0.1% DMSO) control group was set up and transgenic zebrafish Tg(ola.sp7:nlsGFP) was used to select the best concentration for promoting bone formation. The osteoporotic zebrafish were induced by dexamethasone and incubated with nHA and Li-nHA extracts. The wild-type zebrafish was stained with alizarin red and the osteogenic differentiation was observed in transgenic zebrafish. Real-time quantitative PCR was adopted to detect osteogenic maker genes, such as zinc finger transcription factor (SP7), alkaline phosphatase (ALP), osteoprotegerin (OPG), Runt related transcription factor 2(Runx2) and osteocalcin (OCN). Statistical analysis was performed with GraphPad Prism 9.3 software. RESULTS: nHA and Li-nHA promoted bone formation and up-regulated expression levels of ALP, OCN, Runx2, SP7 and OPG of osteoporotic zebrafish. Compared with nHA, Li-nHA significantly increased the mineralization specific staining area and cumulative optical density of zebrafish bone, and the expression of osteogenic maker genes was also significantly increased. CONCLUSIONS: Doping lithium ions in nano hydroxyapatite can enhance its osteoinductive properties, and Li-nHA can effectively improve bone formation of osteoporotic zebrafish. Reference | Related Articles | Metrics

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Effect of collagen sponge on the healing process of alveolar fossa after tooth extraction in rats LI Jun-fu, ZHU Jia, JIANG Juan, WANG Xu-xia, CHEN Dai-yun, ZHAO Zuo-qin Shanghai Journal of Stomatology    2023, 32 (3): 241-245.   DOI: 10.19439/j.sjos.2023.03.004 Abstract （236）      PDF （759KB）（126）       Knowledge map    PURPOSE: To investigate the effect of collagen sponge on early bone healing process of alveolar fossa after tooth extraction in rats. METHODS: A total of 16 healthy female SD rats were selected. Animal models with tooth extraction were established. The right alveolar fossa inserted with collagen sponge was as the experimental group, and the left alveolar fossa was as the control group with treatment. The rats were sacrificed 1, 2, 4 and 8 weeks after tooth extraction, and the osteogenesis of alveolar fossa was observed. Real-time quantitative PCR (qt-PCR) was used to detect the changes of osteogenesis related gene expression. SPSS 19.0 software package was used for statistical analysis. RESULTS: After surgery, alveolar cavity healing was significantly better in the experimental group than in the control group. Osterix, Runx2 and Vegf genes were expressed in the experimental group and the control group, and the expression levels of related genes in the experimental group were significantly higher than the control group 1, 2, 4 and 8 weeks after surgery(P<0.05). CONCLUSIONS: Collagen sponge could promote early alveolar bone healing, possibly related to the expression level of osteogenic genes regulated by collagen sponge. Reference | Related Articles | Metrics

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Applied anatomy of the stylomastoid foramina and its relation to the surrounding bone structures QIN Si-jia, LIU Huan-qi, ZHANG Yu-qing, WANG Dan-yang, HUANG Shuo, WANG Feng, LIU Chang-kui Shanghai Journal of Stomatology    2023, 32 (6): 568-571.   DOI: 10.19439/j.sjos.2023.06.002 Abstract （127）      PDF （607KB）（119）       Knowledge map    PURPOSE: To study the relationship between the stylomastoid foramen and surrounding bony structures, enrich anatomical data and provide reference for clinical surgery. METHODS: A total of 62 intact and dry adult skulls were selected. The shape of the stylomastoid foramen was observed, the diameter of the stylomastoid foramen, the distances from the posterolateral point and the anterior medial point to the surrounding bony structures were measured with a vernier caliper. SPSS 25.0 software package was used to analyze the data. RESULTS: There were four shapes of stylomastoid foramen, i.e., circular (61.29%), oval (29.84%), irregular (8.06%) and triangular (0.81%). The circular diameter was (2.80±0.61) mm, the oval long and short diameters were (4.43±0.96) and (2.79±0.60) mm. Distances from the posterolateral and anterior medial points of the stylomastoid foramen to the posterolateral point of the external opening of the carotid canal, the anterior medial point of the jugular foramen, the midline, the most anterior point of the foramen magnum, the posterior point of the great palatine foramen, the posterolateral point of the foramen lacerated, the foramen ovale, the posterolateral point of the foramen spinosum, the anterior point of the styloid process root, the outermost point of the tympanomastoid fissure and the tip of the mastoid process were (16.10±2.81), (24.01±2.65), (44.95±3.24), (45.10±2.71), (61.66±4.14), (35.56±4.35), (32.26±2.85), (29.12±3.40), (10.39±3.25), (9.49±2.24) and (12.01±2.79) mm; (12.80±2.41), (21.56±2.51), (42.96±3.97), (42.91±2.76), (58.97±3.97), (32.98±4.14), (29.20±2.77), (25.80±2.87), (7.37±2.33), (11.42±2.00) and (15.41±2.57) mm, respectively. Statistical analysis showed that there was no significant difference in the apertures and distances between the left and right side(P>0.05). CONCLUSIONS: Most of the stylomastoid foramen are round and oval, understanding the distance between the foramen and surrounding bony structures is helpful for guiding clinical operations and enriching anatomical knowledge. Reference | Related Articles | Metrics

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Efficacy of early maxillary slow expansion in mixed dentition on maxillary teeth and alveolar crest in the central segment WONG Chung-man, ZHANG Yun, LI Xiao-bing Shanghai Journal of Stomatology    2023, 32 (5): 501-507.   DOI: 10.19439/j.sjos.2023.05.010 Abstract （102）      PDF （1107KB）（116）       Knowledge map    PURPOSE: To study the effect of slow maxillary expansion(SME) appliance on the development of premolar tooth germ, primary molar and alveolar crest in the central segment of maxilla in the mixed dentition. METHODS: According to the inclusion and exclusion criteria, 17 children (average age 7.41±0.80 years old ) with maxillary transverse deficiency were selected for SME. Before and after treatment, based on cone-beam CT(CBCT) and Dolphin Imaging software, the position of maxillary premolar tooth germ, the position of maxillary primary molar, the shape of maxillary alveolar crest in the central segment were analyzed. SPSS 21.0 software package was used for data analysis. RESULTS: There was no significant change in the buccal-lingual movement and inclination of the tooth germs of the maxillary first and second premolars relative to the maxillary alveolar bone cortex after expansion(P>0.05 ). After expansion, the maxillary alveolar crest in the central segment had an obvious expansion effect(P<0.05). There was an arch expansion effect in the alveolar crest bottom of the maxillary second primary molar (P<0.05), but there was no significant difference in the alveolar crest bottom of the maxillary first primary molar before and after treatment. Buccal cusp width of maxillary first and second primary molars increased after arch expansion (P<0.05) without inclination of alveolar crest(P>0.05). CONCLUSIONS: SME in the mixed dentition did not affect the position of premolar tooth germ in alveolar bone. SME can expand and buccally tilt central alveolar crest and dental arch, and buccal inclination is greater than that of skeletal expansion and buccal inclination. The closer tooth site is to the first molar, the greater the skeletal effect of arch expansion is at this site. SME is an effective treatment for early maxillary expansion in the clinic. Reference | Related Articles | Metrics

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Evaluation of the effect of invisible correction and SGTB in the treatment of mandibular retrusion CHEN Shuang-xi, GAO Mei-qin Shanghai Journal of Stomatology    2023, 32 (5): 513-518.   DOI: 10.19439/j.sjos.2023.05.012 Abstract （142）      PDF （1464KB）（115）       Knowledge map    PURPOSE: To compare the clinical effects of invisible correction and SGTB in two-stage treatment of mandibular retrusion. METHODS: Eighty-five patients with bony mandibular regression who did not pass the peak of growth and development were selected. Among them, 40 cases were guided by invisible correction and 45 cases were guided by SGTB functional correction. Lateral head X-rays before and after treatment were measured, and the effect before and after treatment was compared by SPSS 16.0 software package. RESULTS: After treatment, the SNB angle and NP-FH (face angle) increased and the ANB angle decreased in both groups, all of which had significant changes(P<0.001). At T1, the changes of SNB angle, ANB angle and NP-FH angle in the invisible group were smaller than those in the SGTB group(P<0.05). The difference between MP-FH angle and MP-SN angle before and after treatment was greater in the SGTB group than in the invisible group(P<0.001). CONCLUSIONS: In patients with receding mandible before the peak of growth and development, the mandible was significantly moved forward after orthodontics. Both invisible orthodontics and SGTB promote changes in the position of the mandible, improves coordination between the upper and lower jaws. In the control of the posterior teeth in the vertical direction, the invisible group is stronger than the SGTB group. Reference | Related Articles | Metrics

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Current status of oral health literacy and its influencing factors among adult orthodontic patients HU Xiang-ying, WANG Bi-xia, YUAN Wei-jun, ZHU Min, XIAO Yan Shanghai Journal of Stomatology    2023, 32 (5): 545-550.   DOI: 10.19439/j.sjos.2023.05.018 Abstract （94）      PDF （722KB）（114）       Knowledge map    PURPOSE: To investigate the current status of oral health literacy among adult orthodontic patients and analyze its influencing factors. METHODS: Totally 163 adult orthodontic patients were recruited by convenience sampling method from November 2021 to February 2022 at the Department of Oral and Craniomaxillofacial Surgery, Center of Craniofacial Orthodontics in Shanghai Ninth People's Hospital. General information questionnaire, Chinese version of Health Literacy in Dentistry (HeLD-14), Chinese version of Self-Efficacy Scale for Self-care (SESS) and Social Support Rating Scale (SSRS) were applied. SPSS 21.0 software package was used for data analysis. RESULTS: The mean score of oral health literacy in adult orthodontic patients was (48.74±5.54), and the lowest score were obtained in concern (6.80±1.19) and support (6.40±1.59) dimensions. Multiple stepwise regression analysis showed that adult orthodontic patients' consultation self-efficacy, social support utilization, family or friends' orthodontic history and objective support were the influencing factors of oral health literacy. CONCLUSIONS: The oral health literacy in adult orthodontic patients is at a middle to high level, but concern and support literacy need to be improved. Strengthening consultation self-efficacy, objective support and utilization of social support can improve adult orthodontic patients' oral health literacy. Attention should be paid to patients who have no relatives or friends with history of orthodontic treatment. Reference | Related Articles | Metrics

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Effect of polishing method and time on the fitness of CAD/CAM zirconia ceramic crown ZHAO Chen, BAI Yue-hui, LIU Yang, JIANG Shan-shan, SHANG Qing-long, ZHAO Tian-tian, YUAN Shuo, LI Ning, JIAO Jian-ping Shanghai Journal of Stomatology    2023, 32 (3): 236-240.   DOI: 10.19439/j.sjos.2023.03.003 Abstract （231）      PDF （870KB）（114）       Knowledge map    PURPOSE: To compare the effect of different polishing methods and time treatment on the fitness of CAD/CAM zirconia ceramic crowns. METHODS: Sixteen intact maxillary premolars were randomly divided into two groups, group A was treated with silicon carbide burs, while group B was treated with tungsten steel burs. At different polishing time points of the same tooth, digital impressions of each group were obtained, which were used to manufacture CAD/CAM zirconium ceramic crowns. After trial fitting, the gap impressions were obtained by using silicone rubber replication method, and the marginal and internal discrepancies were assessed. The data were statistically analyzed with SPSS 21.0 software package. RESULTS: The difference between the gap values of the marginal and internal markers of group A and group B was not statistically significant(P>0.05). Compared with the no-polishing process, the differences of the marginal gap (39.67±8.35) μm and internal gap (45.18±7.16) μm of group A polished for 4 min, and the marginal gap (51.25±14.73) μm, and internal gap (48.56±6.45) μm of group B polished for 3 min, as well as the marginal gap (48.87±8.90) μm, and internal gap (45.99±7.12) μm of group B polished for 4 min, were all significant(P<0.05). CONCLUSIONS: CAD/CAM zirconia ceramic crowns treated with silicon carbide bur for polishing 4 min and tungsten steel for 3 min has the best fitness. Reference | Related Articles | Metrics

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Imaging findings of carcinoma ex pleomorphic adenoma in minor salivary glands ZHU Wen-Jing, YU Qiang, TAO Xiao-feng, WANG Shao-ying, TIAN Tao, ZHANG Chun-ye Shanghai Journal of Stomatology    2023, 32 (3): 255-260.   DOI: 10.19439/j.sjos.2023.03.007 Abstract （209）      PDF （1392KB）（113）       Knowledge map    PURPOSE: To summarize the CT and MR imaging features of carcinoma ex pleomorphic adenoma(Ca-ex-PA) in minor salivary gland, and analyze the correlation between various features and pathological classification. METHODS: Forty-three patients with Ca-ex-PA in minor salivary gland were collected. The CT and MRI findings were retrospectively analyzed and correlated with their pathological types. Fisher's exact test was used to analyze the correlation between various imaging features (tumor morphology, boundary, internal structure, bone invasion, cervical lymph node metastasis) and pathological types with SPSS 25.0 software package. RESULTS: Among the 43 patients with Ca-ex-PA, 83.7%(36/43) of the tumors were lobulated; 81.4%(35/43) showed cystic degeneration or necrosis, with heterogeneous enhancement. Coarse calcification or mixed calcification was found in 37.2%(16/43), 25.6%(11/43) had compressive absorption of adjacent bone. 75%(12/16) of type Ⅰ/Ⅱ tumors had regular morphology (round or oval), and 77.8%(21/27) of type Ⅲ tumors had irregular morphology, 93.8%(15/16) of type Ⅰ/Ⅱ tumors had well-defined margin and 66.7%(18/27) of type Ⅲ tumors had ill-defined margin. Osteolytic bone resorption occurred in 59.3%(16/27) of type Ⅲ tumors. The average maximum diameter of type Ⅰ/Ⅱ tumors was significantly shorter than that of type Ⅲ(P<0.05). Fisher's exact test showed the characteristics of tumor morphology, boundary and osteolytic bone resorption were related to pathological grouping(P<0.001). CONCLUSIONS: Most Ca-ex-PA in minor salivary glands is characterized by lobular and heterogeneous enhanced neoplasm on CT and MR imaging. A round or oval tumor with well-defined margin usually correlates with typeⅠ and Ⅱ, contrarily, an irregular mass with ill-defined margin and osteolytic bone destruction usually correlates with type Ⅲ. Combining the three characteristics of morphology, boundary and osteolysis is more helpful to distinguish type Ⅰ/Ⅱ and type Ⅲ tumors. Reference | Related Articles | Metrics

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Experimental study of APB-DOCK8 transgenic tomato vaccine for caries prevention LONG Qian, LIAO Cheng-cheng, XIAO Lin-lin, LIU Jian-guo, GUAN Xiao-yan Shanghai Journal of Stomatology    2023, 32 (6): 561-567.   DOI: 10.19439/j.sjos.2023.06.001 Abstract （95）      PDF （1616KB）（111）       Knowledge map    PURPOSE: To observe the anti-caries effect of transgenic tomato anti-caries vaccine after immunization with SD rats by gavage and to explore its immunity mechanism initially. METHODS: SD rats were used to establish an experimental caries model. The transgenic anti-caries tomatoes expressing the target protein were cultivated and identified. The SIgA and IgG contents of specific anti-PAcA in saliva and blood samples of SD rats were detected by ELISA. Then, the SD rats were sacrificed, the maxillary and mandibular bones were taken for Keyes dental caries score, and spleens were taken for the analysis of RNA-seq. Statistical analysis was performed with SPSS 18.0 software package. RESULTS: The target protein concentration in the transgenic tomato anti-caries vaccine was 36.28μg/mL. After vaccine immunization of SD rats, group D (8 mL/kg) produced the highest levels of specific SIgA and IgG antibodies at week 6 and was significantly different from the other groups(P<0.05), and caries counting score was also significantly different than the other groups (P<0.05). The spleen mRNA of SD rats in group D was extracted and sequenced by RNA-seq, and 40 genes with significant differences in mRNA expression were obtained(P-adjust<0.05 & |Fold Change|≥1.5). 26 genes were significantly upregulated, including IGFBP6 and COL15A1. The upregulated gene GO enrichment was enriched to humoral immune response, B-cell activation, and immunoglobulin receptor binding; KEGG enrichment was enriched to 56 signaling pathways, including PI3K-AKT and NF-κB, and F<0.001. Fourteen genes were significantly downregulated, but the analysis of downregulated gene GO and KEGG enrichment was not statistically significant(F>0.1). CONCLUSIONS: Transgenic tomato anti-caries vaccine may reduce caries occurrence by upregulating the activation of PI3K-AKT signaling pathway mediated by IGFBP6 in SD rats. Reference | Related Articles | Metrics

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Accuracy evaluation of static guided implant placement using an intraoral scan method YE Sheng-jia, YANG Xiao-yan, ZHANG Jia-ming, XIN Xian-zhen, WEI Bin, ZENG Li, GONG Yao Shanghai Journal of Stomatology    2023, 32 (5): 491-496.   DOI: 10.19439/j.sjos.2023.05.008 Abstract （116）      PDF （1066KB）（109）       Knowledge map    PURPOSE: To evaluate the accuracy of static guided implant placement with intraoral scanning technology and to analyze the influencing factors of guided surgery. METHODS: Totally 27 cases were included in this retrospective study. The implant designs were made in 3Shape Implant Studio and then guided implant surgeries were performed with CAD-CAM templates. Postoperative implant positions were detected with an intraoral scanner (3Shape TRIOS) and deviation of implantation was evaluated using established CAD/CAM based evaluation method. SAS 9.4 software package was used for data analysis. RESULTS: The mean deviation of entrance point and apical point was (1.182±0.609) mm and (1.658±0.741) mm, respectively. Angular deviation was (5.712±3.347)°. Implant quadrant, location of the implant site, guidance degree, supporting type and implant size influenced direction deviation, while angular deviation was mainly affected by guidance degree and number of missing teeth. CONCLUSIONS: Accuracy of static guided implant placement can be influenced by many factors. More research is needed to improve the accuracy of static guided implantation. Reference | Related Articles | Metrics

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Corilagin-induced apoptosis of oral squamous carcinoma CAL-27 cells in vitro and in vivo and its mechanism LI Sheng-nan, CUI Fei-yan, ZHAO Shan, DU Chen, MENG Jian Shanghai Journal of Stomatology    2023, 32 (5): 462-467.   DOI: 10.19439/j.sjos.2023.05.003 Abstract （100）      PDF （1286KB）（108）       Knowledge map    PURPOSE: To investigate the effect of corilagin on proliferation and apoptosis of human oral squamous cell carcinoma CAL-27 cells, and to explore the molecular mechanism of inducing cell apoptosis. METHODS: In vitro experiments, Cal-27 cells were treated with different concentrations of corilagin, cell-counting kit-8(CCK-8) assay and colony formation assay were performed to evaluate cell proliferation; flow cytometric analysis was used to evaluate cell apoptosis; qRT-PCR and Western blot assays were performed to evaluate the effect of corilagin on the expression levels of Bax, Bcl-2, Caspase-3, Cleaved Caspase-3 in CAL-27 cells. In vivo experiments, tumor-bearing nude mice was constructed with CAL-27 cells to evaluate the antitumor effect of corilagin. GraphPad Prism 8.0 software package was used for statistical analysis of the data. RESULTS: In vitro experiments showed that corilagin in a dose-dependent manner inhibited proliferation, induced apoptosis, up-regulated Bax, caspase-3, cleaved caspase-3 and down-regulated Bcl-2 at the mRNA and protein levels of CAL-27 cells, and the differences were statistically significant(P<0.05). In vivo experiments showed that compared with the control group, corilagin could significantly reduce the volume of tumor in nude mice(P<0.05). CONCLUSIONS: Corilagin can significantly inhibit CAL-27 cell growth and promote its apoptosis both in vitro and in vivo, which may be related to the mediation of Bax/Bcl-2/Caspase-3 signaling pathway. Reference | Related Articles | Metrics

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Effects of let-7a on proliferation, osteogenic differentiation and apoptosis of human dental pulp stem cells LIU Yan, SHAN Dan-ni, SUN Jing, ZOU Yu-xi, YUAN Chang-yong Shanghai Journal of Stomatology    2023, 32 (5): 468-474.   DOI: 10.19439/j.sjos.2023.05.004 Abstract （97）      PDF （2789KB）（106）       Knowledge map    PURPOSE: To study the effect and possible mechanism of let-7a on proliferation, differentiation and apoptosis of human dental pulp stem cell (hDPSCs). METHODS: The cells were divided into four groups: overexpression control (let-7a control/let-7a agomir control), overexpression let-7a (let-7a mimics/let-7a agomir), knockdown let-7a control (let-7a inhibitor control) and knockdown let-7a (let-7a inhibitor). Cell counting kit-8 assay(CCK-8) was used to detect the proliferation of cells at 24 hours, 48 hours and 72 hours after transfection. Calcified nodules were detected by Alizarin red staining. The protein expression of alkaline phosphatase (ALP), osteopontin (OPN), 4E-binding protein 1 (4EBP1), p-4EBP1, mammalian target of rapamycin (mTOR) and p-mTOR were detected by Western blot. Annexin V-APC/7-AAD cell apoptosis detection kit was used to detect the level of apoptosis after transfection. Statistical analysis was performed using GraphPad Prism 5.0 software. RESULTS: Let-7a inhibited proliferation of hDPSCs and promoted odontoblast differentiation and apoptosis. Let-7a down-regulated the expression of 4EBP1, p-4EBP1, mTOR and p-mTOR. CONCLUSIONS: Let-7a may inhibit proliferation of hDPSCs and promote their differentiation and apoptosis by inhibiting mTOR-4EBP1 molecular pathway. Reference | Related Articles | Metrics

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The effect of visual education based on digital intraoral scanning on postoperative anxiety in patients with immediate dental implant WANG Bei, ZHANG Xiao, ZHU Cao-yun Shanghai Journal of Stomatology    2023, 32 (5): 551-554.   DOI: 10.19439/j.sjos.2023.05.019 Abstract （106）      PDF （619KB）（105）       Knowledge map    PURPOSE: To evaluate the effect of visual education based on digital intraoral scanning on postoperative anxiety and treatment confidence in patients with immediate dental implant. METHODS: A total of 266 patients who underwent immediate implantation in Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from January to October 2022 were randomly divided into 2 groups based on the ratio of 1:1, the control group adopted traditional nursing education method, and the experimental group carried out visual education based on digital intraoral scanning on the basis of traditional nursing education. The anxiety self-rating scale (SAS) was used to evaluate the anxiety level after surgery, the numerical score method (NRS) was used to evaluate the patient's confidence in treatment, and visual analogue score(VAS) was used to evaluate the patient's satisfaction with nursing work. SAS 9.4 software package was used for data analysis. RESULTS: After intervention, the SAS scores of the two groups were significantly lower than those before the intervention (P<0.01), and the SAS score of the experimental group was significantly lower than that of the control group(P<0.01). Pearson Chi-square test did not find significant difference between the two groups in the treatment confidence of immediate implantation after intervention(P>0.05), but the results of Mantel-Haenszel Chi-square test showed that the treatment confidence of the experimental group was significantly increased compared with that of the control group (P<0.05). The VAS score of the experimental group for nursing job satisfaction evaluation was significantly higher than that of the control group(P<0.01). CONCLUSIONS: Visual education based on digital intraoral scanning in the immediate implant patient population can significantly improve patients' anxiety, which is conducive to further improving patients' confidence and satisfaction. Reference | Related Articles | Metrics

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Metabolomics study on the difference of tongue coating metabolites between patients with intra-oral halitosis and healthy individual LIMAN Aviella-nathania, ZHU Hong-lai, YANG Wen-jie, YE Wei Shanghai Journal of Stomatology    2023, 32 (5): 525-531.   DOI: 10.19439/j.sjos.2023.05.014 Abstract （117）      PDF （1306KB）（105）       Knowledge map    PURPOSE: To analyze the difference of metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals by untargeted metabolomics, and to explore significant differences in metabolites of intra-oral halitosis as biomarkers. METHODS: The untargeted metabolomics of tongue coating samples from 12 patients with intra-oral halitosis and 12 healthy individuals were studied by liquid chromatography and mass spectrometry combined with principal component analysis and orthogonal partial least squares discriminant analysis. The value of variable importance in projection >1 and P<0.05 of Student's t test in the orthogonal partial least squares-discriminant analysis model were used as the criteria to screen and determine the differential metabolites. RESULTS: There were differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals, and 11 different metabolites were identified. They were valyl-arginine, glycine-phenylalanine, tryptophyl-proline, deoxyadenosine, 4,5-dihydroniveusin A, N-acetyl-DL-tryptophan, paramethasone acetate, cyclopentanol, [(2-hexylcyclopentylidene) amino]thiourea, L-pipecolic acid and taurine. In the intra-oral halitosis group, the expressions of Glycine-phenylalanine and N-acetyl-DL-tryptophan were significantly up-regulated, while the expressions of taurine were significantly down-regulated. CONCLUSIONS: There are differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals. The differential metabolites with diagnostic value may be used as diagnostic markers of intra-oral halitosis. Reference | Related Articles | Metrics

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Oral dexketoprofen tromethamine for preemptive analgesia in extraction of impacted teeth: a randomized controlled double-blind trial ZHAO Jun, WU Xiao-bo, LIU Na, ZHOU Yu, HAO Xin-he Shanghai Journal of Stomatology    2023, 32 (3): 298-301.   DOI: 10.19439/j.sjos.2023.03.015 Abstract （149）      PDF （528KB）（105）       Knowledge map    PURPOSE: To study the preemptive analgesic effect of dexketoprofen tromethamine in extraction of impacted teeth. METHODS: Twenty patients with bilateral mandibular impacted teeth were selected, and were randomly divided into dexketoprofen tromethamine group(experimental group) and placebo group(control group). The pain scores of patients at 0.5, 2, 4, 8, 12, and 24 hours after tooth extraction were counted by numeric rating scale(NRS), and the total dosage of emergent analgesic drugs used in 24 hours was recorded. COX analysis method was used to compare the interval time and the number of cases of first application of emergent analgesic drugs after two operations, and the survival curve was drawn. SPSS 20.0 software package was used for data analysis. RESULTS: The NRS scores of postoperative pain in the experimental group were significantly lower than those in the control group at 2, 4, 8 and 12 hours after operation (P<0.05). The dose of emergent analgesics used in the experimental group for 24 h was significantly lower than that in the control group (P<0.05). Survival curve showed that the interval time between the first application of analgesics in the experimental group was significantly longer than that in the control group(P<0.05). CONCLUSIONS: Dexketoprofen tromethamine can achieve obvious analgesic effect within 12 hours, but the analgesic effect is not obvious after 12 hours. Reference | Related Articles | Metrics