Effect of low magnitude tension on the inflammatory response of periodontal ligament cells induced by isoproterenol

doi:10.19439/j.sjos.2022.03.001

Abstract

Abstract: PURPOSE: To investigate the effect of tension on the inflammatory response of human periodontal ligament cells (PDLCs) induced by isoproterenol (ISO) and its molecular mechanism. METHODS: Human PDLCs were cultured in vitro and stimulated with a certain concentration of ISO(0.01, 0.1, 1 μmol/L) for 24 h. Cyclic tensile strain with different degrees of elongation (5%, 10% and 15%) were applied. The expression of IL-1β and IL-6 mRNA in PDLCs was detected by real-time quantitative PCR(RT-qPCR). The protein expression of p-PERK, PERK, p-eIF2α, eIF2α and ATF4 related to ER stress was detected by Western blot. The expression of PERK gene in PDLCs was knocked down by cell transfection technique, and the expression of IL-1β and IL-6 mRNA in PDLCs with low expression of PERK was detected by RT-qPCR under the stimulation of ISO and low magnitude tension. Statistical analysis was conducted with SPSS 22.0 software package. RESULTS: ISO induction could significantly up-regulate the IL-1β and IL-6 mRNA expression in PDLCs(P<0.05). Compared with the control group, the expression of IL-1β and IL-6 mRNA in PDLCs induced by ISO was inhibited by low magnitude tension, and the difference was statistically significant(P<0.05). Western blot results showed that low magnitude tension could inhibit the ISO-stimulated phosphorylation of PERK and eIF2α and the expression of ATF4（P<0.05）. Compared with the negative control group, IL-1β and IL-6 mRNA expression was decreased in the ISO-stimulated PDLCs silenced by PERK gene. CONCLUSIONS: Tension with 5% degrees of elongation may inhibit ISO-stimulated periodontal inflammatory response through endoplasmic reticulum stress pathway.

Key words: Periodontal ligament cells, Tension, ER stress, Isoproterenol, Inflammatory response

CLC Number:

R781.4

WANG Xue-chun, WU Yan-qi, MEI Peng, ZHANG Bo-jun, ZHU Min. Effect of low magnitude tension on the inflammatory response of periodontal ligament cells induced by isoproterenol[J]. Shanghai Journal of Stomatology, 2022, 31(3): 225-231.

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