Effect of overdose fluoride on the expression of TGF-β3 in rat incisor

doi:10.19439/j.sjos.2018.01.005

Abstract

Abstract: PURPOSE: To observe the effect of overdose fluoride on the expression of TGF-β3 in rat incisor and to explore the possible mechanism of dental fluorosis． METHODS: Twenty Wistar rats were randomly divided into 2 groups. The animals were maintained in standard environmental conditions with free access to food and water (control group) or water added with 100mg/L F (experimental group). The rats were killed at the end of 8th week. The expression of TGF-β3 was detected by immunohistochemical staining. Statistical analysis was performed using SPSS12.0 software package. RESULTS: The expression of TGF-β3 in ameloblasts was significantly inhibited in the experimental of group Ⅱ（P<0.01）. The gray value of the control group and the fluorine group were 85.89±7.90, 116.76±8.04, respectively. CONCLUSIONS: Fluoride might disturb the signal transduction between the epithelia and mesenchyma by inhibiting the expression of TGF-β3 in ameloblasts, which in turn may inhibit the differentiation and function of the tooth-forming cells.

Key words: Fluoride, Rat, TGF-β

CLC Number:

R780.1

LUO Ping-ping, XU Hua-shun. Effect of overdose fluoride on the expression of TGF-β3 in rat incisor[J]. Shanghai Journal of Stomatology, 2018, 27(1): 22-24.

References

[1] Everett ET.Fluoride's effects on the formation of teeth and bones, and the influence of genetics[J]. J Dent Res, 2011, 90(5): 552-560.
[2] Riksen EA, Kalvik A, Brookes S, et al.Fluoride reduces the expression of enamel proteins and cytokines in an ameloblast-derived cell line[J]. Arch Oral Biol, 2011, 56(4): 324-330.
[3] Bronckers AL, Lyaruu DM, DenBesten PK. The impact of fluoride on ameloblasts and the mechanisms of enamel fluorosis[J]. J Dent Res, 2009, 88(10): 877-893.
[4] Zhang Y, Zhang K, Ma L, et al.Fluoride induced endoplasmic reticulum stress and calcium overload in ameloblasts[J]. Arch Oral Biol, 2016, 69: 95-101.
[5] Wang L, Zhu Y, Wang D.High-dose fluoride induces apoptosis and inhibits ameloblastin secretion in primary rat ameloblast[J]. Biol Trace Elem Res, 2016, 174(2): 402-409.
[6] Ripamonti U, Dix-Peek T, Parak R, et al.Profiling bone morphogenetic proteins and transforming growth factor-betas by hTGF-beta3 pre-treated coral-derived macroporous bioreactors: the power of one[J]. Biomaterials, 2015, 49: 90-102.
[7] Lee PW, Yang Y, Racke MK, et al.Analysis of TGF-beta1 and TGF-beta3 as regulators of encephalitogenic Th17 cells: Implications for multiple sclerosis[J]. Brain Behav Immun, 2015,46: 44-49.
[8] Frei K, Gramatzki D, Tritschler I, et al.Transforming growth factor-beta pathway activity in glioblastoma[J]. Oncotarget, 2015, 6(8): 5963-5977.
[9] Thesleff I, Aberg T.Molecular regulation of tooth development[J]. Bone, 1999, 25(1): 123-125.
[10] Guo Q, Liu C, Li J, et al.Gene expression modulation in TGF-beta3-mediated rabbit bone marrow stem cells using electrospun scaffolds of various stiffness[J]. J Cell Mol Med, 2015, 19(7): 1582-1592.
[11] Miyanishi K, Trindade MC, Lindsey DP, et al.Effects of hydrostatic pressure and transforming growth factor-beta 3 on adult human mesenchymal stem cell chondrogenesis in vitro[J]. Tissue Eng, 2006,12(6): 1419-1428.
[12] Suzuki M, Ikeda A, Bartlett JD. Sirt1 overexpression suppresses fluoride-induced p53 acetylation to alleviate fluoride toxicity in ameloblasts responsible for enamel formation [J]. Arch Toxicol, 2017 Nov 28. [Epub ahead of print]
[13] 李健, 张颖, 马林, 等. 氟对大鼠成釉细胞GRP-78和caspase-12表达的影响[J].上海口腔医学, 2015, 24(1): 1-5.
[14] Bègue-Kirn C, Ruch JV, Ridall AL, et al.Comparative analysis of mouse DSP and DPP expression in odontoblasts, preameloblasts, and experimentally induced odontoblast-like cells[J]. Eur J Oral Sci, 1998, 106(Suppl 1): 254-259.
[15] Lane J, Yumoto K, Azhar M, et al.Tak1, Smad4 and Trim33 redundantly mediate TGF-beta3 signaling during palate development[J]. Dev Biol, 2015, 398(2): 231-241.