LncRNA RUNX1-IT1 regulating malignant pleomorphic adenoma via mir-195/CyclinD1

doi:10.19439/j.sjos.2023.01.016

Abstract

Abstract: PURPOSE: To investigate the regulation of long non-coding RNA (LncRNA) RUNX1-IT1 on microrna (mir-195)/CyclinD1 (CyclinD1) in malignant pleomorphic adenoma (MPA). METHODS: The MPA tissues and para-carcinoma tissues were collected and the expression levels of LncRNA RUNX1-IT1, miR-195 and CyclinD1 mRNA were detected, the correlation and clinical pathology of MPA was analyzed and compared. MPA cell line SM-AP1 was cultured and transfected with negative control(NC) siRNA, LncRNA RUNX1-IT1siRNA, miR-NC and miR-195 inhibitor. Cell proliferation level A₄₉₀ and expression levels of miR-195 and CyclinD1 were detected. LncRNA RUNX1-IT1 targeting miR-195 and miR-195 targeting CyclinD1 were analyzed by dual luciferase reporter gene assay. SPSS 21.0 software package was used for data analysis. RESULTS: The expression level of LncRNA RUNX1-IT1 and CyclinD1 in MPA were higher than those in para tumor tissues, and the expression level of miR-195 was lower than that in para tumor tissues(P<0.05). LncRNA RUNX1-IT1was negatively correlated with miR-195, positively correlated with CyclinD1, and miR-195 was negatively correlated with CyclinD1. The expression of LncRNA RUNX1-IT1 and CyclinD1 in MPA tissue with tumor diameter≥3 cm, recurrence and distant metastasis increased(P<0.05), while the expression of miR-195 decreased(P<0.05). After knockdown of LncRNA RUNX1-IT1, A₄₉₀ level and CyclinD1 expression level decreased, while miR-195 expression level increased(P<0.05). miR-195 decreased the fluorescence activity of LncRNA RUNX1-IT1 and CyclinD1 reporter genes(P<0.05). After miR-195 was inhibited, the effect of LncRNA RUNX1-IT1 knockdown on decreasing A₄₉₀ level and CyclinD1 expression level weakened(P<0.05). CONCLUSIONS: LncRNA RUNx1-IT1 may participate in the development of MPA by regulating the expression of miR-195/CyclinD1.

Key words: Malignant pleomorphic adenoma, LncRNA RUNX1-IT1, miR-195, CyclinD1, Proliferation, Target gene

CLC Number:

R739.8

WEI Jun-shui, SUN Xin, XU Jin-biao, CHEN Yi-da. LncRNA RUNX1-IT1 regulating malignant pleomorphic adenoma via mir-195/CyclinD1[J]. Shanghai Journal of Stomatology, 2023, 32(1): 85-90.

References

[1] Sentani K, Ogawa I, Ozasa K, et al.Characteristics of 5 015 salivary gland neoplasms registered in the Hiroshima Tumor Tissue Registry over a period of 39 years[J]. J Clin Med, 2019, 8(5): 566-579.
[2] 王张嵩, 谢舒乐, 张汉卿, 等. 2 456例唾液腺肿瘤临床病理分析[J]. 口腔疾病防治, 2020, 28(5): 298-302.
[3] 魏军水, 卢浩, 杨雯君, 等. 人多形性腺瘤中与cyclin D1共表达LncRNA的初步筛选及鉴定[J]. 中国口腔颌面外科杂志, 2019, 17(3): 198-203.
[4] Sugai T, Osakabe M, Niinuma T, et al.Genome-wide analysis of microRNA and mRNA expression in colorectal intramucosal neoplasia and colorectal cancer with a microsatellite-stable phenotype based on adenoma-carcinoma sequences[J]. Front Oncol, 2022, 12: 831100.
[5] Wu ZB. MiR-195 connects lncRNA RUNX1-IT1 and cyclin D1 to regulate the proliferation of glioblastoma cells[J]. Int J Neurosci, 2021 Feb 3.
[Online ahead of print].
[6] Noel L, Medford S, Islam S, et al.Epidemiology of salivary gland tumours in an Eastern Caribbean nation: a retrospective study[J]. Ann Med Surg (Lond), 2018, 36: 148-151.
[7] Linxweiler M, Wang J, Morris LGT.The role of immune surveillance in malignant transformation of benign salivary gland tumors[J]. Oncotarget, 2021, 12(7): 592-595.
[8] Iida Y, Serizawa M, Mukaigawa T, et al.Molecular profile of a pleomorphic adenoma of the hard palate: a case report[J]. Medicine (Baltimore), 2020, 99(29): e21207-e21210.
[9] Aro K, Valle J, Tarkkanen J, et al.Repeatedly recurring pleomorphic adenoma: a therapeutic challenge[J]. Acta Otorhinolaryngol Ital, 2019, 39(3): 156-161.
[10] 蒋尚飞, 刘洪, 颜成东, 等. PCNA和CyclinD1在腮腺恶性多形性腺瘤组织中的表达和临床意义[J]. 系统医学, 2018, 3(18): 16-19.
[11] Marcolino TF, Pimenta CAM, Artigiani Neto R, et al.p53, Cyclin-D1, beta-catenin, APC and c-myc in tumor tissue from colorectal and gastric cancer patients with suspected lynch syndrome by the bethesda criteria[J]. Asian Pac J Cancer Prev, 2020, 21(2): 343-348.
[12] Ibrahim HM, AbdElbary AM, Mohamed SY, et al. Prognostic value of Cyclin D1 and CD44 expression in gastric adenocarcinoma[J]. J Gastrointest Cancer, 2019, 50(3): 370-379.
[13] Liu S, Zhang J, Yin L, et al.The lncRNA RUNX1-IT1 regulates C-FOS transcription by interacting with RUNX1 in the process of pancreatic cancer proliferation, migration and invasion[J]. Cell Death Dis, 2020, 11(6): 412-428.
[14] Jin M, Wang L, Zheng T, et al.MiR-195-3p inhibits cell proliferation in cervical cancer by targeting BCDIN3D[J]. J Reprod Immunol, 2021, 143: 103211-103220.
[15] Yin Y, Hong S, Yu S, et al.MiR-195 inhibits tumor growth and metastasis in papillary thyroid carcinoma cell lines by targeting CCND1 and FGF2[J]. Int J Endocrinol, 2017: 6180425.
[16] Hao X, Jia Q, Yuan J, et al.MicroRNA-195 suppresses cell proliferation, migration and invasion in epithelial ovarian carcinoma via inhibition of the CDC42/CCND1 pathway[J]. Int J Mol Med, 2020, 46(5):1862-1872.