Changes of cementum endotoxin levels in different teeth with periodontitis treated with root conditioning

doi:10.19439/j.sjos.2017.02.010

Abstract

Abstract: PURPOSES: To observe the changes of endotoxin levels after different teeth with periodontitis were treated with different methods. METHODS: Six healthy premolars extracted for orthodontic reasons and 36 posterior teeth extracted due to severe periodontitis were selected. Each tooth was processed from two 4 mm×4 mm×1 mm cementum pieces 2 mm under the cementum-enamel junction, each tooth with periodontitis was numbered. Healthy teeth served as negative control group, one of the two tablets from each tooth with periodontitis was selected in the periodontitis group, which was not treated with root surface treatment. The remaining 36 teeth with periodontitis were randomly divided into 6 groups: SRP group, SRP + antimicrobial peptide A group , SRP + antimicrobial peptide B group, SRP + EDTA group, SRP + Er:YAG laser group and SRP + Nd:YAG laser group. Endotoxin concentration in each tooth was determined by chromogenic substrate limulus reagent. The endotoxin concentration in each tooth was recorded according to the serial number, and the changes of endotoxin concentration were calculated before and after treatment. SPSS17.0 software package was used to analyze the data. RESULTS: Compared with the teeth with periodontitis, endotoxin concentration decreased to varying degrees, there were significant differences in each treatment group(P<0.01). Compared with SRP group, endotoxin levels in SRP + antimicrobial peptide A group, SRP + antimicrobial peptide B group, SRP + Er:YAG laser group were significantly decreased(P<0.01). No significant difference decreased was from between SRP + EDTA group and SRP + Nd:YAG laser group(P>0.05). CONCLUSIONS: Treatment of teeth with periodontitis using different methods can decrease the level of endotoxin, and the treatment of periodontitis root surface with antimicrobial peptide A + SRP may be more effective.

Key words: Periodontitis, Endotoxin, Cementum, Root conditioning

CLC Number:

R781.4

FU Chang-sheng, LIU Rong-sen, LUO Yun, OU Long, LI Ying-chao, ZHANG Xian-hua, TIAN Lei, DU Yan. Changes of cementum endotoxin levels in different teeth with periodontitis treated with root conditioning[J]. Shanghai Journal of Stomatology, 2017, 26(2): 175-179.

References

[1] 张宇, 艾丽思, 林莉. 牙龈卟啉单胞菌菌体表面毒力因子及其致病性研究进展 [J]. 中国实用口腔科杂志, 2015, 8(1): 43-47.
[2] Yaneva B, Firkova E, Karaslavova E, et al. Bactericidal effects of using a fiber-less Er:YAG laser system for treatment of moderate chronic periodontitis: preliminary results [J]. Quintessence Int, 2014, 45(6): 489-497.
[3] Schwarz F, Bieling K, Venghaus S, et al. Influence of fluorescence-controlled Er:YAG laser radiation, the vector system and hand instruments on periodontally diseased root surfaces in vivo [J]. J Clin Periodontol, 2006, 33(3): 200-208.
[4] Ageitos JM, Sánchez-Pérez A, Calo-Mata P, et al. Antimicrobial peptides (AMPs): Ancient compounds that represent novel weapons in the fight against bacteria [J]. Biochem Pharmacol, 2016 Sep 20.
[5] Garg J, Maurya R, Gupta A, et al. An in vitro scanning electron microscope study to evaluate the efficacy of various root conditioning agents [J]. J Indian Soc Periodontol, 2015, 19(5): 520-524.
[6] Johnson GK, Guthmiller JM, Joly S, et al. Interleukin-1 and interleukin-8 in nicotine- and lipopolysaccharide-exposed gingival keratinocyte cultures [J]. J Periodontal Res, 2010, 45(4): 583-588.
[7] Tsutsumi T, Takeshi T, Nakashima K, et al. Involvement of adhesion molecule in in vitro plaque-like formation of macrophages stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide [J]. J Periodontal Res, 2010, 45(4): 550-556.
[8] Gerhold K, Avagyan A, Reichert E, et al. Lipopolysaccharides modulate allergen-specific immune regulation in a murine model of mucosal tolerance induction [J]. Int Arch Allergy Immunol, 2008, 147(1): 25-34.
[9] 吕娇, 赵文峰. 牙周病非手术治疗的研究进展 [J]. 国际口腔医学杂志, 2016, 43(5): 594-598.
[10] Israel M, Cobb CM, Rossmann JA, et al. The effects of CO 2 , Nd:YAG and Er:YAG lasers with and without surface coolant on tooth root surfaces. An in vitro study [J]. J Clin Periodontol, 1997, 24(9 Pt 1): 595-602.
[11] Crespi R, Capparè P, Toscanelli I, et al. Effects of Er:YAG laser compared to ultrasonic scaler in periodontal treatment: a 2-year follow-up split-mouth clinical study [J]. J Periodontol, 2007, 78(7): 1195-1200.
[12] Kranendonk A, van der Reijden W, van Winkelhoff A, et al. The bactericidal effect of a genius Nd:YAG laser [J]. Int J Dent Hyg, 2010, 8(1): 63-67.
[13] Fritz P, Beck-Jendroschek V, Brasch J. Inhibition of dermatophytes by the antimicrobial peptides human β-defensin-2, ribonuclease 7 and psoriasin [J]. Med Mycol, 2012, 50(6): 579-584.
[14] Crack LR, Jones L, Malavige GN, et al. Human antimicrobial peptides LL-37 and human β-defensin-2 reduce viral replication in keratinocytes infected with varicella zoster virus [J]. Clin Exp Dermatol, 2012, 37(5): 534-543.
[15] Chih YH, Lin YS, Yip BS, et al. Ultrashort antimicrobial peptides with antiendotoxin properties [J]. Antimicrob Agents Chemother, 2015, 59(8): 5052-5056.