The expression and clinical significance of IL-35 in periodontitis and oral lichen planus

Abstract

Abstract: PURPOSE: To investigate the influence of IL-35 in the pathogenesis of periodontitis and oral lichen planus, and the correlation between periodontitis and oral lichen planus patients. METHODS: The gingival crevicular fluid（GCF） and serum were collected from patients with oral lichen planus (n=20), periodontitis (n=20), periodontitis and oral lichen planus (n=20) and healthy controls (n=12). The patients' basic information, probing depth, clinical attachment loss, gingival index, sulcus bleeding index were measured and collected. The expression of IL-35 in GCF and serum was detected by ELISA. SPSS19.0 software package was used for statistical analysis. RESULTS: The concentration of IL-35 in patients with periodontitis and oral lichen planus was significantly higher than that of other groups(P<0.05). There was significantly positive correlation between IL-35 level and periodontal clinical parameters. CONCLUSIONS: Periodontitis and oral lichen planus may increase the concentration of IL-35 both in serum and GCF. The expression of IL-35 was positively correlated with periodontitis and oral lichen planus.

Key words: IL-35, Periodontitis, Oral lichen planus, Serum, Gingival crevicular fluid

CLC Number:

R781.4

WANG Yu, JIN Ying, WANG Yi-yue, XU Dan-ni, LIN Xiao-ping. The expression and clinical significance of IL-35 in periodontitis and oral lichen planus[J]. Shanghai Journal of Stomatology, 2016, 25(4): 443-447.

References

[1] Huang N, Gibson FC 3rd. Immuno-pathogenesis of periodontal disease: current and emerging paradigms [J]. Curr Oral Health Rep, 2014, 1(2): 124-132.
[2] Ertugrul AS, Dursun R, Dundar N, et al. MMP- 1, MMP- 9, and TIMP- 1 levels in oral lichen planus patients with gingivitis or periodontitis[J]. Arch Oral Biol, 2013, 58(7): 843 -852.
[3] López-Jornet P, Camacho-Alonso F. Periodontal conditions in patients with oral lichen planus: a pilot study[J]. Quintessence Int, 2012, 43(2): 147-152.
[4] Ertugrul AS, Arslan U, Dursun R, et al. Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis [J]. Int J Oral Sci, 2013, 5(2): 92 -97.
[5] 王玉, 林晓萍. 口腔扁平苔藓与牙周炎关系的研究进展 [J]. 口腔医学, 2015, 35(1): 71-75.
[6] Zhang D, Wang J, Li Z, et al. The activation of NF-κB in infiltrated mononuclear cells negatively correlates with Treg cell frequency in oral lichen planus[J]. Inflammation, 2015, 38(4): 1683-1689.
[7] Kalburgi NB, Muley A, Shivaprasad BM, et al. Expression profile of IL-35 mRNA in gingiva of chronic periodontitis and aggressive periodontitis patients: a semiquantitative RT-PCR study [J]. Dis Markers, 2015, 35(6): 819-823.
[8] 陈谦明. 口腔黏膜病学[M]. 第4版. 北京:人民卫生出版社, 2012: 103-110.
[9] 孟焕新. 牙周病学 [M]. 第4版. 北京: 人民卫生出版社, 2013: 169-171.
[10] 王琳源, 靳赢, 林晓萍.CD4 + 细胞在不同菌株诱导小鼠牙周炎中的表达 [J]. 上海口腔医学, 2014, 23(1): 20-25.
[11] Ohlrich EJ, Cullinan MP, Seymour GJ. The immunopathogenesis of periodontal disease [J]. Aust Dent J, 2009, 54(Suppl 1): S2-10.
[12] 靳赢, 王琳源, 林晓萍. 实验性牙周炎中调节性T细胞的检测及免疫状态 [J]. 上海口腔医学, 2013, 22(5): 487-491.
[13] Liu JQ, Liu Z, Zhang X, et al. Increased Th17 and regulatory T cell responses in EBV-induced gene 3-deficient mice lead to marginally enhanced development of autoimmune encephalomyelitis [J]. J Immunol, 2012, 188(7): 3099-3106.
[14] Popovska M, Grchevska L, Popovski V, et al. T-cell subpopulations in lesions of oral lichen planus [J]. Prilozi, 2013, 34(2) : 144-150.
[15] Wirtz S, Billmeier U, McHedlidze T, et al. Interleukin-35 mediates mucosal immune responses that protect against T-cell-dependent colitis [J]. Gastroenterology, 2011, 141(5): 1875-1886.
[16] Köseolu S, Salam M, Pekbariyanik T, et al. Level of interleukin-35 in gingival crevicular fluid, saliva and plasma in periodontal disease and health [J]. J Periodontol, 2015, 86(8): 964-971.
[17] Agnihotri R, Gaur S. Rheumatoid arthritis in the elderly and its relationship with periodontitis: a review[J]. Geriatr Gerontol Int, 2014, 14(1): 8-22.
[18] Chee B, Park B, Bartold PM. Periodontitis and type II diabetes: a two-way relationship [J]. Int J Evid Based Healthc, 2013, 11(4): 317-329.
[19] Wang H, Han Q, Luo Z, et al. Oral lichen planus may enhance the expression of Th17- associated cytokines in local lesions of chronic periodontitis [J]. Clin Oral Investig, 2014, 18(6): 1647 -1654.
[20] Salgado DS, Jeremias F, Capela MV, et al. Plaque control improves the painful symptoms of oral lichen planus gingival lesions. A short-term study [J]. J Oral Pathol Med, 2013, 42(10): 728-732.