Regulation effect of serum IFN-γ and IL-4 expression on immune response in patients with oral lichen planus

doi:10.19439/j.sjos.2023.03.010

Abstract

Abstract: PURPOSE: To investigate the regulation effect of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in the serum of patients with oral lichen planus (OLP) on immune response. METHODS: A total of 83 OLP patients in Wangfujing Branch of Beijing Stomatological Hospital, Capital Medical University from January 2019 to December 2021 were selected as the experimental group, and another 61 healthy volunteers who came to the hospital during the same period were included as the control group. The expression levels of IFN-γ and IL-4 in the serum and the expression of immune function indexes of the two groups were compared. The relationship between the expression levels of IFN-γ, IL-4 and immune function indicators in the experimental group was analyzed. SPSS 22.0 software package was used to analyze the data. RESULTS: The levels of IFN-γ and IL-4 in the experimental group were significantly higher than those in the control group(P<0.05). There was no significant difference in the levels of IFN-γ between patients with reticulated OLP and erosive OLP in the experimental group (P>0.05). The level of IL-4 in patients with erosive OLP in the experimental group was significantly higher than that in patients with reticulated OLP(P<0.05). The levels of CD3⁺, CD4, CD16⁺+CD56⁺ and CD4⁺/CD8⁺ in the experimental group were significantly lower than those in the control group(P<0.05), the levels of CD8⁺ and CD19⁺ in the experimental group were significantly higher than those in the control group(P<0.05). The levels of IgG, IgM and CH50 in the experimental group were significantly higher than those in the control group(P<0.05), and the levels of IgA, C3 and C4 in the experimental group were significantly lower than those in the control group(P<0.05). Pearson correlation analysis showed that the level of IFN-γ in the experimental group was negatively correlated with CD16⁺+CD56⁺ and CD4⁺/CD8⁺ (P<0.05), the level of IL-4 in the experimental group was negatively correlated with CD16⁺+CD56⁺ and CD4⁺/CD8⁺(P<0.05), and the level of IL-4 in the experimental group was positively correlated with IgG (P<0.05). CONCLUSIONS: Serum IFN-γ and IL-4 in OLP patients are abnormally high, and IL-4 expression is significantly higher in erosive OLP patients. The cellular and humoral immune functions of OLP patients are disordered and related to the regulation of CD16⁺+CD56⁺, CD4⁺/CD8⁺, and IgG immune responses.

Key words: Oral lichen planus, Interferon-γ, Interleukin-4, Immune response

CLC Number:

R781.5

ZHAO Xiang, GE Hua-bing. Regulation effect of serum IFN-γ and IL-4 expression on immune response in patients with oral lichen planus[J]. Shanghai Journal of Stomatology, 2023, 32(3): 271-275.

References

[1] González-Moles Má, Warnakulasuriya S, González-Ruiz I, et al.Worldwide prevalence of oral lichen planus: a systematic review and meta-analysis[J]. Oral Dis, 2021, 27(4): 813-828.
[2] Villa TG, Sánchez-Pérez á, Sieiro C.Oral lichen planus: a microbiologist point of view[J]. Int Microbiol, 2021, 24(3): 275-289.
[3] Gholizadeh N, Sheykhbahaei N.Micronutrients profile in oral lichen planus: a review literature[J]. Biol Trace Elem Res, 2021, 199(3): 912-924.
[4] Liao H, Luo Y, Long L, et al.Anxiety and oral lichen planus[J]. Oral Dis, 2021, 27(3): 506-514.
[5] 中华口腔医学会口腔黏膜病学专业委员会, 中华口腔医学会中西医结合专业委员会. 口腔扁平苔藓诊疗指南(修订版)[J]. 中华口腔医学杂志, 2022, 57(2):115-121.
[6] Zhu ZD, Ren XM, Zhou MM, et al.Salivary cytokine profile in patients with oral lichen planus[J]. J Dent Sci, 2022, 17(1): 100-105.
[7] Duan L, Liu D, Chen H, et al.Follicular dendritic cells restrict interleukin-4 availability in germinal centers and foster memory B cell generation[J]. Immunity, 2021, 54(10): 2256-2272.
[8] 刘健, 刘晓敏, 闫佩佩, 等. 口腔扁平苔藓与NF-κB通路相关因子IL-8和RANTES的关系研究[J]. 北京口腔医学, 2020, 28(2): 78-81.
[9] Yang JY, Tan YQ, Zhou G.T cell-derived exosomes containing cytokines induced keratinocytes apoptosis in oral lichen planus[J]. Oral Dis, 2022, 28(3): 682-690.
[10] 中华口腔医学会口腔黏膜病专业委员会,中华口腔医学会中西医结合专业委员会, 周红梅, 等. 口腔扁平苔藓诊疗指南(试行)[J]. 中华口腔医学杂志, 2012, 47(7): 399-401.
[11] Bukhari AF, Farag AM, Treister NS.Chronic oral lesions[J]. Dermatol Clin, 2020, 38(4): 451-466.
[12] Lin D, Yang L, Wen L, et al.Crosstalk between the oral microbiota, mucosal immunity, and the epithelial barrier regulates oral mucosal disease pathogenesis[J]. Mucosal Immunol, 2021, 14(6): 1247-1258.
[13] Sun A, Wu YH, Chang JY, et al.FoxP3+CD4+, IFN-γ+CD4+, and IFN-γ+CD8+ cell levels in erosive and non-erosive types of oral lichen planus patients[J]. J Dent Sci, 2021, 16(2):751-756.
[14] Tarsariya VM, Mehta DN, Raval N, et al.Evaluation of serum immunoglobulin (IgG, IgM, IgA) in potentially malignant disorders of oral cavity - a case control study[J]. J Oral Biol Craniofac Res, 2020, 10(4): 665-669.
[15] Tan YQ, Wang F, Ma RJ, et al.Interferon-γ activated T-cell IRGM-autophagy axis in oral lichen planus[J]. Int Immunopharmacol, 2021, 94(3): 107478-107483.
[16] Mehrbani SP, Motahari P, Azar FP, et al.Role of interleukin-4 in pathogenesis of oral lichen planus: a systematic review[J]. Med Oral Patol Oral Cir Bucal, 2020, 25(3): e410-e415.