Expression of OPG/RANK/RANKL in the rat dental pulp tissue of periodontitis combined with vascular calcification and its clinical significance

Abstract

Abstract: PURPOSE: To study the expression and possible role of OPG/RANK/RANKLin the rat dental pulp of periodontitis combined with vascular calcification. METHODS: Thirty-six male Wister rats were randomly divided into 4 groups: control group(group C), periodontitis group(group CP), vascular calcification group(group VDN) and compound group(group CP+VDN). Each group underwent corresponding management to establish animal model. When the model was successful, the maxillae including molars were sectioned, pulp tissue was examined by H-E staining; Immunohistochemical staining method was used to evaluate the expression and ratio of OPG and RANKL in pulp tissues. Statistical analysis was carried out using SPSS 19.0 software package. RESULTS: The pulp tissue of group CP, VDN, CP+VDN showed varied degrees of damage, neutrophil infiltration, pulp vascular congestion, odontoblasts vacuolar changes, pulp necrosis by H-E staining, and the changes in CP+VDN group was the most significant, followed by CP group, VDN group. Immunohistochemistry showed OPG in pulp tissues in group CP, VDN, CP+VDN were significantly lower than that in normal group (P<0.05), and the expression in group CP+VDN was the least;Expression of RANKL in pulp tissues in group CP, VDN, CP+VDN were significantly higher than that in normal group(P<0.05),and the expression in group CP+VDN was the highest. The ratio of OPG/RANKL in normal group was the highest, and the ratio in CP+VDN group was the lowest. CONCLUSIONS: Periodontitis and vascular calcification can damage the pulp tissue, periodontitis compound with vascular calcification may aggravate the injury; OPG/RANKL/RANK system may play an important role in pulp tissue injury.

Key words: Periodontitis, Vascular calcification, Pulp tissue, OPG/RANK/RANKL

CLC Number:

R781.4

PAN Ke-qing, ZHANG Peng-mei, DENG Jing, LOU Xiu-xiu, MENG Yun, LIU Gui-rong. Expression of OPG/RANK/RANKL in the rat dental pulp tissue of periodontitis combined with vascular calcification and its clinical significance[J]. Shanghai Journal of Stomatology, 2016, 25(4): 391-395.

References

[1] Beck JD, Eke P, Heiss G, et al. Periodontal disease and coronary heart disease: a reappraisal of the exposure [J]. Circulation, 2005, 112(1): 19-24.
[2] Glodny B, Nasseri P, Crismani A, et al. The occurrence of dental caries is associated with atherosclerosis[J]. Clinics (Sao Paulo), 2013, 68(7): 946-953.
[3] Li JJ, Zhu CG, Yu B, et al. The role of inflammation in coronary artery calcification [J]. Ageing Res Rev, 2007, 6(4): 263-270.
[4] 孟芸, 潘克清, 张朋梅, 等. 炎性因子在大鼠牙周炎合并血管钙化模型中的表达 [J]. 青岛大学医学院学报, 2015, 51(4): 480-482.
[5] 章立群, 阮毅, 陈绛媛, 等. 慢性牙周炎患牙牙髓中T、B淋巴细胞的免疫组化研究 [J]. 中山大学学报, 2008, 29(S2): 41-43.
[6] 刘琪, David Haynes,Mark Bartold. 慢性牙周炎中核因子κ B受体活化子、护骨素和肿瘤坏死因子α的蛋白表达[J]. 口腔医学, 2004, 24(6): 347-349.[7] RANi CS, MacDougall M. Dental cells express factors that regulate bone resorption[J]. Mol Cell Bio Res Commun, 2000, 3(3): 145-152.
[8] 李小彤, 张丁, 傅民魁, 等. 机械牵张力对人牙周膜细胞成骨样细胞功能的影响 [J]. 中华口腔医学杂志, 2002, 37(2): 135-138.
[9] Moqi M, Otogoto J, Ota N, et al. Differential expression of RANKL and osteoprotegerin in gingival crevicular fluid of patients with periodontitis [J]. J Dent Res, 2004, 83(2): 166-169.
[10] 唐倩, 吴坚, 梁焕友, 等. 龈沟液中核因子活化因子受体配体和骨保护素水平的检测[J].中国现代医学杂志, 2005, 15(18): 2773-2776.
[11] Mohammadpour AH, Shamsara J, Nazemi S, et al. Evaluation of RANKL/OPG serum concentration ratio as a new biomarker for coronary artery calcification: a pilot study[J]. Trombosis, 2012:306263.
[12] Ozkok A, Caliskan Y, Sakaci T, et al. Ostoprotegerin/RANKL axis and progression of coronary artery calcification in hemodialysis patients[J]. Clin J Am Soc Nephrol, 2012, 7(6): 965-973.
[13] Morena M, Dupuy AM, Jaussent I, et al. A cut-off value of plasma osteoprotegerin level may predict the presence of coronary artery calcifications in chronic kidney disease patients[J]. Nephrol Dial Transplant, 2009, 24(11): 3389-3397.
[14] Ohmori R, Momiyama Y, Taniguchi H, et al. Association between osteoprotegerin genepolymorphism and coronary artery disease in Japanese men [J]. Atherosclerosis, 2006, 187(1): 215-217.
[15] Jian B, Narula N, Li QY, et al. Progression of aortic valve stenosis: TGF-beta l is present in calcified aortic valve cusps and promotesaortic valve interstitial cell calcification via apoptosis [J]. Ann Thorac Surg, 2003, 75(2): 457-465.
[16] Meneguini M, Regalia A, Alfieri C, et al. Calcium and osteoprotegerin levels predict the progression of the abdominal aortic calcifications after kidney transplantation [J]. Transplantation, 2013, 96(1): 42-48.
[17] 刘继中, 纪宗玲, 陈苏民. OPG/RANKL/RANK系统与骨破坏性疾病 [J]. 生物工程学报, 2003, 19(6): 655-660.
[18] Niederhoffer N, Bobryshev YV, Lartaud - Idjouadiene I, et al. Aortic calcification produced by vitamin D3 plus nicotine [J]. Vasc Res, 1997, 34(5): 386-398.
[19] 姜帅, 邓婧, 刘桂荣, 等. 慢性牙周炎合并血管钙化大鼠实验模型的建立 [J].牙体牙髓牙周病学杂志, 2014, 24(5): 278-282.
[20] Lu HK, Chen YL, Chang HC, et al. Identification of the osteoprotegerin/receptor activator of nuclear factor-kappa B ligand system in gingival crevicular fluid and tissue of patients with chronic periodontitis [J]. J Periodontal Res, 2006, 41(4): 354-360.