The efficacy of cold-light tooth bleaching on the formation of bacteria biofilm on the enamel surfaces

Abstract

Abstract: PURPOSE: To evaluate the efficacy of Beyond cold-light tooth bleaching on the formation of main cariogenic bacteria biofilm on enamel surfaces. METHODS: Twenty enamel discs with the size of 4 mm×4 mm×1 mm in size, were made .The enamel discs were divided into 4 groups randomly: cold-light bleaching group, bleaching gel group, cold-light group and control group. Five discs were in each group. Cold-light bleaching group was whitened 3 times with bleaching gel and cold-light, and 12 min per session. Bleaching gel was smeared on the surface of enamel in bleaching gel group for 3 times and 12 min per session. Enamel discs of cold-light group were treated with cold-light for 12 min and 3 sessions. Control group was treated without any processing. The 4 groups were incubated in mixed bacteria liquid, including Streptococcus mutans(SM), Actinomyces viscosus (Av) and Fusobacterium nucleatum (Fn), within the artificial oral cavity model. After 36 h, the samples were observed under confocal laser scanning microscopy(CLSM). The data was analyzed with SAS8.2 software package. RESULTS: The biofilms in 3 experimental groups were sparser than the control group under CLSM, and the thickness significantly decreased after treatment (P<0.05), while no significant difference was found among 3 experimental groups (P>0.05).Compared with the control group, the percentage of vital bacteria in biofilm of the experimental groups decreased significantly after treatment (P<0.001). CONCLUSIONS: Cold-light tooth bleaching can inhibit the formation of mixed bacteria biofilm, damage the structure of biofilm and reduce the number of vital bacteria.

Key words: Cold-light bleaching, Dental enamel, Streptococcus mutans, Actinomyces viscosus, Fusobacterium nucleatum, Biofilm

CLC Number:

R781.1

YUAN Ke-yong,MA Rui,ZHU Cai-lian,LI Ming-yu,HE Zhi-yan,ZHOU Wei. The efficacy of cold-light tooth bleaching on the formation of bacteria biofilm on the enamel surfaces[J]. Shanghai Journal of Stomatology, 2015, 24(3): 283-287.

References

[1] Kwon SR, Wertz PW, Li Y, et al. Penetration pattern of rhodamine dyes into enamel and dentin: confocal laser microscopy observation[J]. Int J Cosmet Sci, 2012, 34(1): 97-101.
[2] Toledano M, Yamauti M, Osorio E, et al. Bleaching agents increase metalloproteinases-mediated collagen degradation in dentin [J]. J Endod, 2011, 37(12): 1668-1672.
[3] Tredwin CJ, Naik S, Lewis NJ, et al. Hydrogen peroxide tooth-whitening(bleaching) products: review of adverse effects and safety Issues [J]. Br Dent J, 2006, 200(7): 371-376.
[4] Chan W. Summary of: side effects of external tooth bleaching: a multi-centre practice-based prospective study [J]. Br Dent J, 2013, 215(9): 466-467.
[5] Hosoya N, Honda K, Iino F, et al. Changes in enamel surface roughness and adhesion of Streptococcus mutans to enamel after vital bleaching [J]. J Dent, 2003, 31(8): 543-548.
[6] 郑春艳,潘洁,王祖华,等.过氧化物美白剂对釉质表面变形链球菌的生物膜的影响[J].北京大学学报(医学版),2014,46(1):30-34.
[7] Ittatirut S, Matangkasombut O, Thanyasrisung P. In-office bleaching gel with 35% hydrogen peroxide enhanced biofilm formation of early colonizing Streptococci on human enamel [J].J Dent, 2014, 42(11): 1480-1486.
[8] 朱敏, 刘正, 李鸣宇, 等. 简易人工口腔的建立[J]. 中华口腔医学杂志, 1996, 31(2): 110-112.
[9] Krzyciak W, Jurczak A, Kocielniak D, et al. The virulence of Streptococcus mutans and the ability to form biofilms [J]. Eur J Clin Microbiol Infect Dis, 2014, 33(4): 499-515.
[10] Aas JA, Paster BJ, Stokes LN, et al. Defining the normal bacterial flora of the oral cavity [J]. J Clin Microbiol, 2005, 43(11): 5721- 5732.
[11] Whittaker CJ, Klier CM, Kolenbrander PE. Mechanisms of adhension by oral bacteria[J]. Annu Rev Microbiol,1996,50: 513-552.
[12] Kolenbrander PE, London J. Adhere today, here tomorrow: oral bacterial adherence [J]. J Bacteriol, 1993, 175(11): 3247-3252.
[13] Abu-Shakra A, Zeiger E. Effects of Salmonella genotypes and testing protocols on H 2 O 2 -induced mutation[J]. Mutagenesis, 1990, 5(5): 469-473.
[14] Feuerstein O, Ginsburg I, Dayan E, et al. Mechanism of visible light phototoxicity on Porphyromonas gingivalis and Fusobacterium nucleatum [J]. Photochem Photobiol, 2005, 81(5): 1186-1189.
[15] Feuerstein O, Persman N, Weiss EI. Phototoxic effect of visible light on Porphyromonas gingivalis and Fusobacterium nucleatum: an in vitro study [J]. Photochem Photobiol, 2004, 80(3): 412-415.
[16] Feuerstein O, Moreinos D, Steinberg D. Synergic antibacterial effect between visible light and hydrogen peroxide on Streptococcus mutans [J]. J Antimicrob Chemother, 2006, 57(5): 872-876.
[17] Shi XC, Ma H, Zhou JL, et al. The effect of cold-light-activated bleaching treatment on enamel surfaces in vitro [J]. Int J Oral Sci, 2012, 4(4): 208-213.
[18] Unlu N, Cobankara FK, Ozer F. Effect of elapsed time following bleaching on the shear bond strength of composite resin to enamel [J]. J Biomed Mater Res, 2008, 84(2): 363-368.