Exploring the stem cell surface markers expressed in human dental pulp stem cells

Abstract

Abstract: PURPOSE: To explore the stem cell surface markers expressed in human dental pulp stem cells which were selected and isolated by magnetic beads. METHODS: Human dental pulp cells (hDPCs) were separated and cultured from dental pulp of healthy third molars for orthodontic purpose. HDPSCs were isolated from cultured hDPCs by magnetic-activated cell sorting’s (MACS) indirect magnetic cell labeling and positive selection strategy with antibody STRO-1 in the 2nd generation. Then the stem cell surface markers (CD73, CD90, CD105, CD166 and STRO-1) were respectively detected in 3, 4, 5, 6, 7 and 8 generation of dental pulp stem cells. HDPSCs were induced to differentiation by adipogenic medium and osteogenic medium in the 3rd generation. Adipogenic differentiation was assessed by oil red O staining in day 21, and osteogenic differentiation was assessed by alizarin red staining in day 21. RESULTS: HDPSCs could differentiate into adipocyte and osteoblasts. Oil red O staining and alizarin red staining were positively expressed after induction of HDPSCs. STRO-1’s expression was decreased with the increase of generation. The expressions of CD73, CD90, CD105 and CD166 were relatively stable. CONCLUSIONS: The expression of STRO-1 is declined with the increase of generation, and the expressions of CD73, CD90, CD105 and CD166 are relatively stable with the changes of generation.

Key words: Human dental pulp stem cells, Magnetic-activated cell sorting, Mesenchymal stem cells, Surface markers

CLC Number:

R781.3

GAO Li,JIANG Wen-xin,NIU Cheng-guang,HE Zhi-yan,ZHU Cai-lian,HUANG Zheng-wei. Exploring the stem cell surface markers expressed in human dental pulp stem cells[J]. Shanghai Journal of Stomatology, 2015, 24(3): 257-262.

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