Comparison of chemotaxis of different concentrations of SDF1,bFGF,IL-8 on bone marrow mesenchymal stem cell

Abstract

Abstract: PURPOSE: To investigate chemotaxis of different concentrations of chemokines stromal cell-derived factor-1 (SDF-1), basic fibroblast growth factor (bFGF) and interleukin-8 (IL-8）on bone marrow mesenchymal stem cells (BMSCs). METHODS: The experiment group was divided into 3 groups according to different culture media: SDF1, bFGF and IL-8. The chemokine concentrations of each group was 0 ng/mL (control concentration),10 ng/mL, 50 ng/mL, 100 ng/mL, 200ng/mL and 400 ng/mL (experimental concentration). BMSCs were isolated from SD rats and cultured in vitro. 24-well plates with an 8 μm pore size polycarbonate membrane were used to examine the chemotaxis of SDF1, bFGF, IL-8 on BMSCs, respectively, and to obtain the best chemotactic concentrations of 3 chemokines. The data was analyzed with SPSS 16.0 software package. RESULTS: Compared with 0 ng/mL group, all chemokines could chemoattract BMSCs (P<0.05). At the concentration of 50 ng/mL, 100 ng/mL and 400 ng/mL, SDF1 significantly recruited more cells than bFGF (P<0.05); SDF1 significantly recruited more cells than IL-8 at the concentration of 200 ng/mL; The best chemotactic concentration was 100 ng/mL for SDF1 and IL-8 and 200 ng/mL for bFGF. CONCLUSIONS: The use of SDF1,bFGF,IL-8 can chemoattract BMSCs. The chemotaxis of SDF1 and IL-8 are better than bFGF.

Key words: SDF1, BFGF, IL-8, BMSCs, Chemotaxis

CLC Number:

R780.2

LIU Yu-jian,WANG Jun,CHI Zheng-bin. Comparison of chemotaxis of different concentrations of SDF1,bFGF,IL-8 on bone marrow mesenchymal stem cell[J]. Shanghai Journal of Stomatology, 2015, 24(3): 275-279.

References

[1] Jones GN, Moschidou D, Lay K, et al. Upregulating CXCR4 in human fetal mesenchymal stem cells enhances engraftment and bone mechanics in a mouse model of osteogenesis imperfecta [J]. Stem Cells Transl Med, 2011, 1(1): 70-78.
[2] Doerks T, Copley RR, Schultz J, et al. Systematic identification of novel protein domain families associated with nuclear functions [J]. Genome Res, 2002, 12(1): 47-56.
[3] Cui X, Chopp M, Zacharek A, et al. Chemokine, vascular and therapeutic effects of combination Simvastatin and BMSC treatment of stroke [J]. Neurobiol Dis, 2009, 36(1): 35-41.
[4] Theiss HD, Vallaster M, Rischpler C, et al . Dual stem cell therapy after myocardial infarction acts specifically by enhanced homing via the SDF-1/CXCR4 axis[J]. Stem Cell Res, 2011, 7(3): 244-255.
[5] Ghadge SK, Mühlstedt S, Ozcelik C, et al. SDF-1α as a therapeutic stem cell homing factor in myocardial infarction [J]. Pharmacol Ther, 2011, 129(1): 97-108.
[6] Lau TT, Wang DA. Stromal cell-derived factor-1(SDF-1): homing factor for engineered regenerative medicine [J]. Expert Opin Biol Ther, 2011, 11(2): 189-197.
[7] Kitaori T, Ito H, Schwarz EM, et al. Stromal cell-derived factor 1/CXCR4 signaling is critical for the recruitment of mesenchymal stem cells to the fracture site during skeletal repair in a mouse model [J]. Arthritis Rheum, 2009, 60(3): 813-823.
[8] Carr AN, Howard BW, Yang HT, et al. Efficacy of systemic administration of SDF-1 in a model of vascular insufficiency: support for an endothelium-dependent mechanism [J].Cardiovasc Res, 2006, 69(4): 925-935.
[9] Kerfoot SM, Andonegui G, Bonder CS, et al. Exogenous stromal cell-derived factor-1 induces modest leukocyte recruitment in vivo [J]. Am J Physiol Heart Circ Physiol, 2008, 294(6): H2524-2534.
[10] Tasso R, Gaetani M, Molino E, et al. The role of bFGF on the ability of MSC to activate endogenous regenerative mechanisms in an ectopic bone formation model [J]. Biomaterials, 2012, 33(7): 2086-2096.
[11] Schmidt A, Ladage D, Schinkothe T, et al. Basic fibroblast growth factor controls migration in human mesenchymal stem cells [J]. Stem Cells, 2006, 24(7): 1750-1758.
[12] Kim SM, Kim DS, Jeong CH, et al. CXC chemokine receptor 1 enhances the ability of human umbilical cord blood-derived mesenchymal stem cells to migrate toward gliomas [J]. Biochem Biophys Res Commun, 2011, 407(4): 741-746.
[13] Suzuki T, Lee CH, Chen M, et al. Induced migration of dental pulp stem cells for in vivo pulp regeneration [J]. J Dent Res, 2011, 90(8): 1013-1018.
[14] Zhai S, Wang Y, Jiang W, et al. Nemotic human dental pulp fibroblasts promote human dental pulp stem cells migration [J]. Exp Cell Res, 2013, 319(10): 1544-1552.
[15] 栾庆先, 涉谷俊昭, 牧克教, 等. 牙龈成纤维细胞、牙周膜细胞对白细胞介素8的趋化反应[J]. 中华口腔医学杂志, 2001, 36(1): 40-41.
[16] Cao Q, Ding P, Lu J, et al. 2’, 3’-Cyclic nucleotide 3’-phosphodiesterase cells derived from transplanted marrow stromal cells and host tissue contribute to perineurial compartment formation in injured rat spinal cord[J]. J Neurosci Res,2007,85(1): 116-130.
[17] Liu X, Zhou C, Li Y, et al. SDF-1 promotes endochondral bone repair during fracture healing at the traumatic brain injury condition [J]. PLoS One, 2013, 8(1): e54077.
[18] Campbell LM, Maxwell PJ, Waugh DJ, et al. Rationale and means to target pro-Inflammatory interleukin-8 (CXCL8) signaling in cancer [J]. Pharmaceuticals (Basel), 2013, 6(8): 929-959.
[19] Heidemann J, Ogawa H, Dwinell MB, et al. Angiogenic effects of interleukin 8 (CXCL8) in human intestinal microvascular endothelial cells are mediated by CXCR2 [J]. J Biol Chem, 2003, 278(10): 8508-8515.