Naringenin promotes osteogenic differentiation of BMSCs via SDF-1α/CXCR4 signaling axis

doi:10.19439/j.sjos.2021.06.004

Abstract

Abstract: PURPOSE: To explore the influence of naringenin on osteogenic differentiation of bone mesenchymal stem cells(BMSCs), and the role of SDF-1α/CXCR4 signaling axis in the osteogenic differentiation by naringenin. METHODS: BMSCs of the rats were isolated,cultured and tested. CCK-8 assay was used to explore the proliferation ability of BMSCs in different concentrations of naringenin, and alkaline phosphatase(ALP) activity was detected. RT-qPCR was used to detect the mRNA expression of ALP, OCN, CXCR4 and SDF-1α in different groups. The expressions of CXCR4 and SDF-1α protein in BMSCs during osteogenic differentiation in different experimental groups were detected by ELISA. SPSS 21.0 software package was used for statistical analysis of the data. RESULTS: The results of cell identification showed that the cultured cells were BMSCs. At 1 d and 3 d, all concentrations of naringenin had no significant effect on the proliferation of BMSCs; and at 5 d, 50 μg/mL of naringenin promoted proliferation of BMSCs;furthermore, at 7 d, all concentrations of naringenin promoted proliferation of BMSCs(P<0.05). ALP activity value gradually increased in each concentration over time. From the RT-qPCR experiment, the mRNA expression of ALP, OCN, CXCR4 and SDF-1α in the naringenin group and the osteogenic induction group was significantly increased compared with the medium group(P<0.05). ELISA assay showed that the protein expressions of CXCR4 and SDF-1α increased gradually in the four groups as time went on and the expression of two proteins was the highest in 100 μg/mL naringenin group. CONCLUSIONS: Naringenin can promote the proliferation and osteogenic differentiation of BMSCs. SDF-1α/CXCR4 signaling axis is involved in the osteogenic differentiation of BMSCs by naringenin,particularly in the early stage of BMSCs osteogenic differentiation.

Key words: BMSCs, Naringenin, SDF-1α/CXCR4, Bone regeneration

CLC Number:

R780.2

BAI Shu-lin, WANG Yi-pei, JIN Ke, LUO Xiao-ling, XU Xiao-mei. Naringenin promotes osteogenic differentiation of BMSCs via SDF-1α/CXCR4 signaling axis[J]. Shanghai Journal of Stomatology, 2021, 30(6): 579-584.

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