In vitro study of electrospun polycaprolactone/chitosan nano fiber membrane guided bone regeneration

Abstract

Abstract: PURPOSE: To explore the effect of electrospining chitosan/polycaprolactone nano fiber membrane on regeneration of bone defects in vitro. METHODS: Chitosan/ polycaprolactone nanofiber membrane was prepared by electrospinning technique. According to alkaline phosphatase activity and Western blot results, the ability of osteogenic differentiation of cells by fiber membrane as carrier was evaluated. The data was analyzed statistically with SPSS17.0 software package. RESULTS: The osteogenic differentiation capacity of electrospining chitosan/polycaprolactone nano fiber membrane was significantly higher than the control (without materials), and the difference was significant (P<0.05). CONCLUSIONS: Electrospinning chitosan/polycaprolactone nano fiber membrane can promote osteogenic differentiation of cells in vitro, which provides a scientific basis for clinical regeneration of bone defects.

Key words: Electrospinning, Chitosan, Polycaprolactone, Osteogenic differentiation

CLC Number:

R783.1

HE Ying,LIU Xin,TANG Xu-yan. In vitro study of electrospun polycaprolactone/chitosan nano fiber membrane guided bone regeneration[J]. Shanghai Journal of Stomatology, 2015, 24(2): 129-134.

References

[1] 王杏松. 口腔修复膜材料在牙种植中引导骨再生中的应用分析 [J]. 中国医药指南, 2014, 12(4): 172-173.
[2] Pontoriero R, Lindhe J, Nyman S, et al. Guided tissue regeneration in degreeⅡfurcation-involved mandibular molars. A clinical study [J]. J Clin Periodontol, 1988, 15(4): 247-254.
[3] Xue J, He M, Niu Y, et al. Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nano fiber membrane [J]. Int J Pharm, 2014, 475(1-2): 566-577.
[4] Xu C, Lei C, Meng L, et al. Chitosan as a barrier membrane material in periodontal tissue regeneration[J]. J Biomed Mater Res B Appl Biomater, 2012, 100(5): 1435-1443.
[5] Chang KY, Cheng LW, Ho GH, et al. Fabrication and characterization of poly (gamma-glutamic acid)-graft-chondroitin sulfate/polycaprolactone porous scaffolds for cartilage tissue engineering [J]. Acta Biomater, 2009, 5(6): 1937-1947.
[6] Zuo Y, Yang F, Wolke JG, et al. Incorporation of biodegradable electrospun fibers into calcium phosphate cement for bone regeneration [J]. Acta Biomater, 2010, 6(4): 1238-1247.
[7] Gupta D, Venugopal J, Mitra S, et al. Nanostructured biocomposite substrates by electrospinning and electrospraying for the mineralization of osteoblasts[J]. Biomaterials, 2009, 30(11): 2085-2094.
[8] 范利梅, 唐旭炎, 李全利, 等.钛表面胞外基质涂层对骨髓基质干细胞增殖和分化的影响 [J]. 安徽医科大学学报, 2009, 44(5): 551-553.
[9] Kim JH, Choung PH, Kim IY, et al. Electrospun nanofibers composed of poly (ε-caprolactone) and polyethylenimine for tissue engineering applications [J]. Mater Sci Eng C, 2009, 29(5): 1725-1731.
[10] Haider S, Park SY. Preparation of the electrospun chitosan nanofibers and their applications to the adsorption of Cu(II) and Pb(II) ions from an aqueous solution[J]. J Memb Sci,2009,328(1-2): 90-96.
[11] Yang X, Chen X, Wang H. Acceleration of osteogenic differentiation of preosteoblastic cells by chitosan containing nanofibrous scaffolds[J]. Biomacromolecules, 2009, 10(10): 2772-2778.
[12] Ohara N, Hayashi Y, Yamade S, et al. Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular[J]. Biomaterials, 2004, 25(10): 1074-1078 .
[13] Hong JH, Hwang ES, McManus MT, et al. TAZ, a transcriptional modulator of mesenehymal stem cell differentiation[J]. Science, 2005, 309 (5737): 1074-1078.
[14] Jadlowiec J, Koch H, Zhang X, et al. Phosphophoryn regulates the gene expression and differentiation of NIH3T3, MC3T3-E1, and human mesenchymal stem cells via the integrin MAPK signaling pathway [J]. J Biol Chem, 2004, 279(51): 53323-53330.
[15] Chang L, Karin M. Mammalian MAP kinase signaling cascades[J]. Nature, 2001, 410(6824): 37-40.
[16] Jaiswal RK, Jaiswal N, Bruder SP, et al. Adult human mesenchymal stem cell differentiation to the osteogenic lineage is regulated by mitogen-activated protein kinase[J]. J Biol Chem, 2000, 275(13): 9645-9652.
[17] Diniz P, Shomura K, Soejima K, et al. Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts [J]. Bioelectromagnetics, 2002, 23(5): 398-405.
[18] 马慧, 赵红斌. Runx 2蛋白与成骨细胞分化信号 [J]. 医学综述, 2007, 13(24): 1959-1962.
[19] Prratap J, Galindo M, Zaidi SK, et al. Cell growth regulatory role of Runx2 during proliferative expansion of preosteoblasts[J]. Cancer Res, 2003, 63(17): 5357-5362.
[20] Seol YJ, Lee JY, Park YJ, et al. Chitosan sponges as tissue engineering scaffolds for bone formation[J]. Biotechnol Lett, 2004, 26(13): 1037-1041.