The effect of additional polyphosphazene nanotubes in polyurethane composites on microorganisms adhesion

Abstract

Abstract: PURPOSE: To investigate Candida albicans and Streptococcus mutans adhesion to 3 polyurethane composites [pure polyurethane (PU), 1% polyphosphazene-containing polyurethane (PZS-1%) and 5% polyphosphazene-containing polyurethane (PZS-5%)]. METHODS: Two kinds of microorganism suspension were incubated with the specimens under certain culture conditions and the roughness of 3 materials were determined before adhesion tests. Microorganism attached to the specimen were then observed under optical microscope, and viable micro-organisms adhered to the specimen were then counted and removed. The data was analyzed with SAS 8.02 softwaree package. RESULTS: PU has the roughest surface followed by PZS-1% and PZS-5%. Most amounts of cell adhesion were observed in PU followed by PZS-1% and least amounts were found in PZS-5%. CONCLUSIONS: The increase of polyphosphazene nanotubes in polyurethane composites may have an effect on cell adhesion. Surface roughness of the materials may be one of the factors.

Key words: Polyurethane, Polyphosphazene, Candida albicans, Streptococcus mutans

CLC Number:

R783.1

KONG Fang-yuan, ZHENG Yuan-li, LI Jing. The effect of additional polyphosphazene nanotubes in polyurethane composites on microorganisms adhesion[J]. Shanghai Journal of Stomatology, 2014, 23(4): 419-422.

References

[1] Murata H, Hamada T, Sadamori S. Relationship between viscoelastic properties of soft denture liners and clinical efficacy[J]. Jap Dent Sci Rev, 2008, 44(2): 128-132.
[2] 王安莉. 影响义齿软衬材料理化性能的因素 [J]. 中国组织工程研究与临床康复, 2008, 12(19): 3717-3720.
[3] Craig RG, Powers JM, Wataha JC. Dental materials. Properties and manipulation [M]. 11th Ed. St. Louis: Mosby, 2002: 668.
[4] Kiat-Amnuay S, Khan Z, Gettleman L. Overdenture retention of four resilient liners over an implant bar[J]. J Prosthet Dent, 1999, 81(5): 568-573.
[5] Pavan S, Arioli Filho JN, Dos Santos PH, et al. Effect of disinfection treatments on the hardness of soft denture liner materials [J]. J Prosthodont, 2007, 16(2): 101-106.
[6] Radford DR, Sweet SP, Challacombe SJ, et al. Adherence of Candida albicans to denture-base materials with different surface finishes [J]. J Dent, 1998, 26(7): 577-583.
[7] 邓华颉, 李玉玲, 陶人川. 白色念珠菌对4种软衬材料表面黏附的比较研究 [J]. 中华老年口腔医学杂志, 2007, 5(2): 90-92.
[8] Ferreira MA, Pereira-Cenci T, Rodrigues de Vasconcelos LM, et al. Efficacy of denture cleaners on denture liners contaminated with Candida species [J]. Clin Oral Invest, 2009, 13(2): 237-242.
[9] Quirynen M, Bollen CM, Papaioannou W, et al. The influence of titanium abutment surface roughness on plaque accumulation and gingivitis: short-term observations [J]. Int J Oral Maxillofac Implants, 1996, 11(2): 169-178.
[10] 沈海军, 史友进. 纳米抗菌材料的分类、制备、抗菌机理及其应用 [J]. 中国粉体工业, 2006, 3(2): 18-20.
[11] 荣兴民, 黄巧云, 陈雯莉, 等. 土壤矿物与微生物相互作用的机理及其环境效应 [J]. 生态学报, 2008, 28(1): 376-387.
[12] B?rgers R, Hahnel S, Reichert TE, et al. Adhesion of candida albicans to various dental implant surfaces and the influence of salivary pellicle proteins[J]. Acta Biomater, 2010, 6(6): 2307-2313.
[13] Hahnel S, Rosentritt M, Handel G, et al. In vitro evaluation of artifecial ageing on surface properties and early Candida albicans adhesion to prosthetic resins[J]. J Mater Sci Mater Med,2009,20(1): 249-255.
[14] Kanie T, Tomita K, Tokuda M, et al. Mechanical properties and cytotoxicity of experimental soft lining materials based on urethane acrylate oligomers [J]. Dent Mater J, 2009, 28(4): 501-506.

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