Relationship between oral Candida albicans and flora in children with severe early childhood caries

doi:10.19439/j.sjos.2021.02.009

Abstract

Abstract: PURPOSE: To investigate the relationship between oral Candida albicans and flora in children with severe infant caries. METHODS: Forty-two children with severe infant caries (experimental group) and 40 caries-free children (control group) treated in Peking University Shenzhen Hospital from March 2018 to June 2019 were enrolled. The samples of saliva and plaque were collected and cultured. According to the culture results, the experimental group was further divided into two subgroups: Candida albicans positive group and Candida albicans negative group. The samples were sequenced by high-throughput sequencing technology, then the diversity and abundance of bacteria were analyzed. The data were processed by SPSS 23.0 software package. RESULTS: The positive rates of Candida albicans in saliva and plaque were significantly higher in the experimental group than in the control group(P<0.05). The abundance of Leptotrichia and Cardiobacterium in the saliva of children with positive Candida albicans in the experimental group was significantly higher than that of children with negative Candida albicans(P<0.05). The abundance of Prevotella in plaque of children with positive Candida albicans in the experimental group was significantly higher than that of children with negative Candida albicans and control group(P<0.05). The abundance of Leptotrichia in saliva of children with positive Candida albicans in the experimental group was significantly higher than that of children with negative Candida albicans (P<0.05), while the abundances of Capnocytophaga, Cardiobacterium hominis, and Capnocytophaga granulose were significantly lower than those of children with negative Candida albicans(P<0.05). The abundance of Streptococcus mutans, Lactobacillus gasseri, and Lactobacillus vaginalis in the plaque of positive Candida albicans in the experimental group were significantly higher than those of children with negative Candida albicans(P<0.05), while the abundance of Lactobacillus plantarum was significantly lower than that of children with negative Candida albicans (P<0.05). CONCLUSIONS: Candida albicans in children with severe infant caries children is closely correlated with the abundance of Leptotrichia, Capnocytophaga, Cardiobacterium hominis, Capnocytophaga granulosa, Streptococcus mutans and Lactobacillus, which may play a synergistic or antagonistic role.

Key words: Severe infant caries, Candida albicans, Flora, Bacterial diversity, Bacterial abundance

CLC Number:

R781.5

YAO Yao, HE Liu-ting. Relationship between oral Candida albicans and flora in children with severe early childhood caries[J]. Shanghai Journal of Stomatology, 2021, 30(2): 156-161.

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